FlyBase Allele Report: Dmel\AMPKα[JF01951]

General Information

Symbol

Dmel\AMPKα^JF01951

Species

D. melanogaster

Name

FlyBase ID

FBal0219937

Feature type

allele

Associated gene

Dmel\AMPKα

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01951}

Also Known As

UAS-AMPK RNAi, TRiP.JF01951, UAS-AMPK RNAi #2

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

(Perkins et al., 2009.1.15)

Progenitor genotype

Carried in construct

P{TRiP.JF01951}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.1.15)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01951

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Li et al., 2016)

model of Parkinson's disease

CEA

(Hang et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates obesity

modeled by Hsap\SNCAIP^UAS.cLa

(Liu et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased efficacy of memory | adult stage | calorie restriction conditional, with Scer\GAL4^GMR54H02

(Silva et al., 2022)

lethal - all die during P-stage, with Scer\GAL4^Act.PU

(Onyenwoke et al., 2015)

lethal - all die during P-stage, with Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

Phenotype Manifest In

adult brain | calorie restriction conditional, with Scer\GAL4^GMR54H02

(Silva et al., 2022)

lipid droplet | adult stage | calorie restriction conditional, with Scer\GAL4^GMR54H02

(Silva et al., 2022)

Detailed Description

Statement

Reference

Adults expressing AMPKα^JF01951 for 2 days under the combined control of Scer\GAL4^GMR54H02 and Gal80[ts] (for temporal control of expression) exhibit decreased olfactory memory under starvation conditions, but not when fed. Starvation induces enlarged lipid droplets in the brain region where cortex glia enwrap mushroom body neuronal soma.

(Silva et al., 2022)

Expression of AMPKα^JF01951 under the control of either Scer\GAL4^tub.PU or Scer\GAL4^Act.PU results in lethality in late pupal stage with no eclosing adults.

(Onyenwoke et al., 2015)

Expression of AMPKα^JF01951 in the fat body under the control of Scer\GAL4^DcG results in significant hyperglycaemia.

(Ugrankar et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

AMPKα^JF01951, Scer\GAL4^Tub.PU has lethal - all die during P-stage phenotype, suppressible | partially by S6k^KQ.UAS, Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

AMPKα^JF01951, Scer\GAL4^Tub.PU has lethal - all die during P-stage phenotype, suppressible | partially by mTor^TED.UAS, Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

AMPKα^JF01951, Scer\GAL4^Act.PU has lethal - all die during P-stage phenotype, suppressible | partially by Trpml¹

(Onyenwoke et al., 2015)

AMPKα^JF01951, Scer\GAL4^Act.PU has lethal - all die during P-stage phenotype, suppressible | partially by Trpml²

(Onyenwoke et al., 2015)

AMPKα^JF01951, Scer\GAL4^Act.PU has lethal - all die during P-stage phenotype, suppressible | partially by Trpml^JF01239, Scer\GAL4^Act.PU

(Onyenwoke et al., 2015)

NOT suppressed by

Statement

Reference

AMPKα^JF01951, Scer\GAL4^Tub.PU has lethal - all die during P-stage phenotype, non-suppressible by Trpml^UAS.cVa, Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

AMPKα^JF01951, Scer\GAL4^Tub.PU has lethal - all die during P-stage phenotype, non-suppressible by SNF4Aγ^UAS.GFP, Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

Suppressor of

Statement

Reference

AMPKα^JF01951, Scer\GAL4^elav.PU is a suppressor | partially of increased rate of feeding behavior | adult stage phenotype of Hsap\SNCAIP^UAS.cLa, Scer\GAL4^elav.PU

(Liu et al., 2021)

AMPKα^JF01951, Scer\GAL4^elav.PU is a suppressor | partially of increased body weight | adult stage phenotype of Hsap\SNCAIP^UAS.cLa, Scer\GAL4^elav.PU

(Liu et al., 2021)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The late pupal lethality observed in animals expressing AMPKα^JF01951 under the control of Scer\GAL4^tub.PU can be partially rescued by co-expression of either S6k^KQ.Scer\UAS or Tor^TED.Scer\UAS but not by co-expression of either Trpml^Scer\UAS.cVa or SNF4Aγ^{Scer\UAS.T:Avic\GFP}.

The late pupal lethality observed in animals expressing AMPKα^JF01951 under the control of Scer\GAL4^Act.PU can be partially rescued by combination with either Trpml¹ or Trpml² or by co-expression of Trpml^JF01239.

(Onyenwoke et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

AMPKα^JF01951 is rescued by AMPKα^UAS.cMa/Scer\GAL4^Tub.PU

(Onyenwoke et al., 2015)

Comments

The late pupal lethality observed in animals expressing AMPKα^JF01951 under the control of Scer\GAL4^tub.PU can be fully rescued by co-expression of AMPKα^Scer\UAS.cMa.

(Onyenwoke et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25931

y¹ v¹; P{TRiP.JF01951}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

AMPKα^JF01951

AMPKα^TRiP.JF01951

(Liu et al., 2021)

SNF1A^JF01951

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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