FlyBase Allele Report: Hsap\APP[Aβ42.E693G.UAS.VTR]

General Information

Symbol

Hsap\APP^{Aβ42.E693G.UAS.VTR}

Species

H. sapiens

Name

FlyBase ID

FBal0256664

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-APP.Aβ42.E693G.VTR}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Singh and Mahoney, 2011.2.17)

Progenitor genotype

Carried in construct

P{UAS-APP.Aβ42.E693G.VTR}

Cytology

Description

UAS drives expression of the 42 amino acid Aβ fragment from Hsap\APP carrying the familial Alzheimer's "Arctic" mutation (E693G - amino acid numbering based on Hsap\APP sequence).

(Singh and Mahoney, 2011.2.17)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease 1

CEC

(Oyarce-Pezoa et al., 2023, Huang et al., 2022, Demirev et al., 2019, Tower et al., 2019)

CEA

(Sharma et al., 2025, Panchal and Tiwari, 2020, Yeh et al., 2018, Ray et al., 2017, Breda et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Alzheimer's disease 1

is ameliorated by brp^+t83.4

(Huang et al., 2022)

is ameliorated by v^KK108195

(Breda et al., 2016)

is exacerbated by Jra^JF01184

(Ray et al., 2017)

is exacerbated by Stat92E^GD4492

(Ray et al., 2017)

is exacerbated by drpr^Δ5

(Ray et al., 2017)

is ameliorated by drpr^I.UAS

(Ray et al., 2017)

is exacerbated by drpr^RNAi.UAS

(Ray et al., 2017)

is exacerbated by kay^JF01380

(Ray et al., 2017)

is ameliorated by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}

(Demirev et al., 2019)

is NOT ameliorated by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}

(Demirev et al., 2019)

is ameliorated by Miro^UAS.cGa

(Panchal and Tiwari, 2020)

is exacerbated by Akt^HM04007

(Sharma et al., 2025)

is ameliorated by Akt^UAS.Exel

(Sharma et al., 2025)

is exacerbated by InR^NIG.18402R

(Sharma et al., 2025)

is ameliorated by InR^UAS.cUa

(Sharma et al., 2025)

is ameliorated by foxo^JF02734

(Sharma et al., 2025)

is exacerbated by foxo^UASp.GFP

(Sharma et al., 2025)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: "Alzheimer's disease 1" is associated with human APP.

(Demirev et al., 2019)

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Singh and Mahoney, 2011.2.17)

This construct carries the amyloid Aβ42 peptide; variant described relative to the full-length protein and in terms of the Aβ42 peptide.

APP:p.Glu693Gly

(FlyBase curators, 2019-)

Variants Synonym(s)

APP(Aβ42 peptide):p.Glu22Gly

Associated human disease model(s)

External database links

ClinVar: APP_18098

Comments concerning this variant

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Demirev et al., 2019)

lobe system of adult mushroom body, with Scer\GAL4^elav-C155

(Demirev et al., 2019)

ommatidium | adult stage, with Scer\GAL4^GMR.PU

(Demirev et al., 2019)

rhabdomere of eye photoreceptor cell | decreased number, with Scer\GAL4^GMR.PU

(Oyarce-Pezoa et al., 2023)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has short lived phenotype, enhanceable by Pld3^KK107515, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has abnormal adult locomotory behavior | progressive phenotype, enhanceable by Pld3^KK107515, Scer\GAL4^elav-C155

(Demirev et al., 2019)

NOT Enhanced by

Statement

Reference

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of long-term memory | adult stage phenotype, non-enhanceable by Pld3^KK107515, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of short-term memory | adult stage phenotype, non-enhanceable by Pld3^KK107515, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has abnormal adult locomotory behavior | progressive phenotype, non-enhanceable by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of long-term memory | adult stage phenotype, non-enhanceable by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^Mef2.247 has decreased efficacy of short-term memory | adult stage phenotype, non-enhanceable by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^Mef2.247

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^Mef2.247 has decreased efficacy of long-term memory | adult stage phenotype, non-enhanceable by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^Mef2.247

(Demirev et al., 2019)

Suppressed by

Statement

Reference

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has abnormal adult locomotory behavior | progressive phenotype, suppressible | partially by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PU

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PU

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of short-term memory | adult stage phenotype, suppressible by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of long-term memory | adult stage phenotype, suppressible by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of short-term memory | adult stage phenotype, suppressible by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^Mef2.247 has decreased efficacy of short-term memory | adult stage phenotype, suppressible by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^Mef2.247

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^Mef2.247 has decreased efficacy of long-term memory | adult stage phenotype, suppressible by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^Mef2.247

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible | partially by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible | partially by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

NOT suppressed by

Statement

Reference

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has short lived phenotype, non-suppressible by brp^+t83.4

(Huang et al., 2022)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of long-term memory | adult stage phenotype, non-suppressible by Pld3^KK107515, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of short-term memory | adult stage phenotype, non-suppressible by Pld3^KK107515, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has abnormal adult locomotory behavior | progressive phenotype, non-suppressible by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has decreased efficacy of long-term memory | adult stage phenotype, non-suppressible by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^Mef2.247 has decreased efficacy of short-term memory | adult stage phenotype, non-suppressible by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^Mef2.247

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^Mef2.247 has decreased efficacy of long-term memory | adult stage phenotype, non-suppressible by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^Mef2.247

(Demirev et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has lobe system of adult mushroom body phenotype, non-enhanceable by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Suppressed by

Statement

Reference

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^GMR.PU has rhabdomere of eye photoreceptor cell | decreased number phenotype, suppressible by DEF8^JF02943, Scer\GAL4^GMR.PU

(Oyarce-Pezoa et al., 2023)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PU

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^GMR.PU has ommatidium | adult stage phenotype, suppressible by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PU

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PU

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^GMR.PU has ommatidium | adult stage phenotype, suppressible | partially by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^GMR.PU

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has lobe system of adult mushroom body phenotype, suppressible by Hsap\PLD3^{UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

NOT suppressed by

Statement

Reference

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Scer\GAL4^elav-C155 has lobe system of adult mushroom body phenotype, non-suppressible by Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155

(Demirev et al., 2019)

Other

Statement

Reference

Hsap\APP^{Aβ42.E693G.UAS.VTR}, Hsap\PLD3^{V232M.UAS.Tag:FLAG,Tag:HA}, Scer\GAL4^elav-C155 has adult Kenyon cell phenotype

(Demirev et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

33774

w¹¹¹⁸; P{UAS-APP.Aβ42.E693G.VTR}8

33773

y¹ w¹¹¹⁸; P{UAS-APP.Aβ42.E693G.VTR}4

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Aβ42^arc

(Demirev et al., 2019)

Hsap\APP^{Aβ42.E693G.Scer\UAS.VTR}

Hsap\APP^{Aβ42.E693G.UAS.VTR}

Name Synonyms

Secondary FlyBase IDs

References (17)

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