FlyBase Allele Report: Dmel\Miro[UAS.cGa]

General Information

Symbol

Dmel\Miro^UAS.cGa

Species

D. melanogaster

Name

FlyBase ID

FBal0239860

Feature type

allele

Associated gene

Dmel\Miro

Associated Insertion(s)

Carried in Construct

P{UAS-Miro.G}

Also Known As

UAS-Miro, UAS-dMiro

Key Links

Transgenic product class

cDNA

(Guo et al., 2005)

wild_type

(Guo et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Guo et al., 2005)

wild_type

(Guo et al., 2005)

Mutagen

in vitro construct

(Guo et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Miro.G}

Cytology

Description

Expression of Miro cDNA (longest splice form) is under the control of UAS sequences.

(Guo et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Miro

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Vagnoni et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Pink1^B9

(Liu et al., 2012)

exacerbates neuromuscular disease

modeled by Hsap\TRPV4^{R269C.UAS.BID}

(Woolums et al., 2020)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.E693G.UAS.VTR}

(Panchal and Tiwari, 2020)

modeled by Hsap\APP^{C99.UAS.Exel.Tag:MYC}

(Panchal and Tiwari, 2020)

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.wt

(Panchal and Tiwari, 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^dpr1-PGaw

(Vagnoni et al., 2016)

axon | larval stage, with Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

dopaminergic neuron | adult stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

embryonic/larval motor neuron | larval stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

epithelium, with Scer\GAL4^Gug-AGiR

(Sew et al., 2025)

larval multidendritic neuron, with Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

marginal nerve | adult stage, with Scer\GAL4^dpr1-PGaw

(Vagnoni et al., 2016)

mitochondrion, with Scer\GAL4^Gug-AGiR

(Sew et al., 2025)

mitochondrion & neuromuscular junction | supernumerary | larval stage, with Scer\GAL4^elav-C155

(Guo et al., 2005)

mitochondrion | adult stage, with Scer\GAL4^dpr1-PGaw

(Vagnoni et al., 2016)

mitochondrion | adult stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

mitochondrion | larval stage, with Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

mitochondrion | larval stage, with Scer\GAL4^ple.PF

(Liu et al., 2012)

neuronal cell body | larval stage, with Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

wing disc, with Scer\GAL4^Gug-AGiR

(Sew et al., 2025)

Detailed Description

Statement

Reference

Expression of Miro^Scer\UAS.cGa under the control of Scer\GAL4^dpr1-PGaw strongly reduces the number of mitochondria that undergo axonal transport in both anterograde and retrograde directions in the wing marginal nerve, as compared to controls.

(Vagnoni et al., 2016)

Expression of Miro^Scer\UAS.cGa under the control of Scer\GAL4^ple.PF causes aberrant mitochondrial aggregation in dopaminergic neurons, and dopaminergic neuron loss, in adults.

Expression of Miro^Scer\UAS.cGa under the control of Scer\GAL4^ple.PF in larval motor neurons results in decreased flux in axonal mitochondrial transport in both anterograde and retrograde directions, increased velocity of anterograde (but not retrograde) mitochondrial transport, increased mitochondrial length, and accumulation of mitochondria in the most distal boutons of neuromuscular junctions, as compared with controls.

(Liu et al., 2012)

Miro^Scer\UAS.cGa; Scer\GAL4^elav-C155 larvae show excessive accumulation of mitochondria at the neuro-muscular junctions

(Guo et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Miro^UAS.cGa, Scer\GAL4^ple.PF has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Pink1^B9/Pink1^B9

(Liu et al., 2012)

Enhancer of

Statement

Reference

Miro^UAS.cGa, Scer\GAL4^CCAP.PU is an enhancer of visible | adult stage phenotype of Hsap\TRPV4^R269C.UAS, Scer\GAL4^CCAP.PU

(Woolums et al., 2020)

Miro^UAS.cGa, Scer\GAL4^ppk.PU is an enhancer of abnormal neuroanatomy | larval stage | progressive phenotype of Hsap\TRPV4^R269C.UAS, Scer\GAL4^ppk.PU

(Woolums et al., 2020)

Scer\GAL4^Mhc.PU/Miro^UAS.cGa is an enhancer of visible phenotype of Pink1^B9

(Liu et al., 2012)

Scer\GAL4^Mhc.PU/Miro^UAS.cGa is an enhancer of abnormal flight phenotype of Pink1^B9

(Liu et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^unspecified/Miro^UAS.cGa is a suppressor | partially of abnormal neurophysiology | somatic clone | adult stage phenotype of αCOP²⁷⁵⁰

(Maddison et al., 2023)

Scer\GAL4^unspecified/Miro^UAS.cGa is a suppressor | partially of abnormal neuroanatomy | somatic clone | adult stage | progressive phenotype of αCOP²⁷⁵⁰

(Maddison et al., 2023)

Other

Statement

Reference

Hsap\TRPV4^{R269C.UAS.BID}, Miro^UAS.cGa, Scer\GAL4^CCAP.PU has visible | adult stage phenotype

(Woolums et al., 2020)

Miro^UAS.cGa, Pink1^B9, Scer\GAL4^ple.PF has partially lethal phenotype

(Liu et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Miro^UAS.cGa, Scer\GAL4^109(2)80 has mitochondrion | larval stage phenotype, non-enhanceable by Sod1^G85R

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80 has larval multidendritic neuron phenotype, non-enhanceable by Sod1^G85R

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80 has axon | larval stage phenotype, non-enhanceable by Sod1^G85R

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^ple.PF has dopaminergic neuron | adult stage phenotype, non-enhanceable by Pink1^B9/Pink1^B9

(Liu et al., 2012)

NOT suppressed by

Statement

Reference

Miro^UAS.cGa, Scer\GAL4^109(2)80 has mitochondrion | larval stage phenotype, non-suppressible by Sod1^G85R

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80 has larval multidendritic neuron phenotype, non-suppressible by Sod1^G85R

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80 has axon | larval stage phenotype, non-suppressible by Sod1^G85R

(Nemtsova et al., 2023)

Enhancer of

Statement

Reference

Miro^UAS.cGa, Scer\GAL4^CCAP.PU is an enhancer of wing phenotype of Hsap\TRPV4^R269C.UAS, Scer\GAL4^CCAP.PU

(Woolums et al., 2020)

Miro^UAS.cGa, Scer\GAL4^ppk.PU is an enhancer of larval multidendritic class IV neuron | wandering third instar larval stage phenotype of Hsap\TRPV4^R269C.UAS, Scer\GAL4^ppk.PU

(Woolums et al., 2020)

Miro^UAS.cGa, Scer\GAL4^ppk.PU is an enhancer of axon | wandering third instar larval stage phenotype of Hsap\TRPV4^R269C.UAS, Scer\GAL4^ppk.PU

(Woolums et al., 2020)

Scer\GAL4^Mhc.PU/Miro^UAS.cGa is an enhancer of wing phenotype of Pink1^B9

(Liu et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^unspecified/Miro^UAS.cGa is a suppressor | partially of endoplasmic reticulum | somatic clone | adult stage | decreased number phenotype of αCOP²⁷⁵⁰

(Maddison et al., 2023)

Scer\GAL4^109(2)80/Miro^UAS.cGa is a suppressor of mitochondrion | larval stage phenotype of Sod1^G85R

(Nemtsova et al., 2023)

Scer\GAL4^109(2)80/Miro^UAS.cGa is a suppressor of larval multidendritic neuron phenotype of Sod1^G85R

(Nemtsova et al., 2023)

Scer\GAL4^109(2)80/Miro^UAS.cGa is a suppressor of axon | larval stage phenotype of Sod1^G85R

(Nemtsova et al., 2023)

Scer\GAL4^109(2)80/Miro^UAS.cGa is a suppressor of synapse | larval stage phenotype of Sod1^G85R

(Nemtsova et al., 2023)

NOT Suppressor of

Statement

Reference

Scer\GAL4^unspecified/Miro^UAS.cGa is a non-suppressor of axon | somatic clone | adult stage phenotype of αCOP²⁷⁵⁰

(Maddison et al., 2023)

Scer\GAL4^unspecified/Miro^UAS.cGa is a non-suppressor of mitochondrion | somatic clone | adult stage | decreased number phenotype of αCOP²⁷⁵⁰

(Maddison et al., 2023)

Scer\GAL4^unspecified/Miro^UAS.cGa is a non-suppressor of combined L1-L3 nerve | somatic clone | adult stage phenotype of αCOP²⁷⁵⁰

(Maddison et al., 2023)

Other

Statement

Reference

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has axon | larval stage phenotype

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has synapse | larval stage phenotype

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has mitochondrion | larval stage phenotype

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has neuronal cell body | larval stage phenotype

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has larval multidendritic neuron phenotype

(Nemtsova et al., 2023)

Hsap\TRPV4^{R269C.UAS.BID}, Miro^UAS.cGa, Scer\GAL4^CCAP.PU has wing phenotype

(Woolums et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Individuals co-expressing Hsap\TRPV4^{R269C.UAS.BID} and Miro^UAS.cGa frequently fail to expand their wings after eclosion.

(Woolums et al., 2020)

Expression of Miro^Scer\UAS.cGa under the control of Scer\GAL4^Mhc.PU enhances the abnormal wing posture and flight defects of Pink1^B9/Pink1^B9 mutants.

Expression of Miro^Scer\UAS.cGa under the control of Scer\GAL4^ple.PF in a Pink1^B9/Pink1^B9 mutant background does not result in a significant difference in dopaminergic neuron number as compared to in a wild-type background, but does result in reduced viability.

(Liu et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^elav-C155/Miro^UAS.cGa partially rescues Miro^B682

(Guo et al., 2005)

Fails to rescue

Scer\GAL4^how-24B/Miro^UAS.cGa fails to rescue Miro^B682

(Guo et al., 2005)

Comments

Miro^Scer\UAS.cGa; Scer\GAL4^elav-C155 rescues most of the phenotypes due to Miro^B682/Miro^B682 : larval lethality, larval body and muscle size, distribution of mitochondria to the distal extremities of neurons, neuro-muscular junction morphology and microtubule cytoskeleton organization. However, distribution of mitochondria within somatic muscle is not rescued in these animals. None of the phenotypes rescued by Miro^Scer\UAS.cGa; Scer\GAL4^elav-C155 are rescued by Miro^Scer\UAS.cGa; Scer\GAL4^how-24B.

(Guo et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51646

w^*; P{UAS-Miro.G}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Miro^Scer\UAS.cGa

Miro^UAS.cGa

Name Synonyms

Secondary FlyBase IDs

References (12)

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