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General Information
Symbol
Dmel\drprdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0258016
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-draperRNAi, pUAST-draper-RNAi, UAS-drprRNAi, UAS-drprRNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a 502bp fragment (nucleotides 1885-2386) of drpr cloned in an inverted repeat in the pWIZ vector.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing drprdsRNA.Scer\UAS in all glia under the control of Scer\GAL4repo are unable to clear axonal debris from L1 wing vein neurons following axotomy. Expression in wrapping glia under the control of Scer\GAL4nrv2.PS also results in strong suppression of clearance of axonal debris, whereas expression in subperineurial glia (under the control of Scer\GAL4Gli-rL82) has a much more modest effect.

Expression of drprdsRNA.Scer\UAS under the control of Scer\GAL4alrm.PD results in a strong suppression of mushroom body γ neuron pruning at 18 hours after pupal formation and a mild phenotype in adults.

drpr appears to function in non-astrocyte glia (e.g. ensheathing or cortex glia), since knockdown of drpr, through expression of drprdsRNA.Scer\UAS under the control of Scer\GAL4alrm.PD, has no effect on cell body clearance.

Follicle cell specific expression of drprdsRNA.Scer\UAS under the control of Scer\GAL4GR1 prevents the follicle cell enlargement and uptake of nurse cell debris normally seen in starvation-induced degenerating egg chambers.

Expression of drprScer\UAS.dsRNA in the hemocytes under the control of Scer\GAL4Hml.PG does not result in defects in salivary gland clearance.

Expression of drprScer\UAS.dsRNA in the salivary glands under the control of Scer\GAL4fkh.PH results in persistence of salivary gland fragments in 89% of pupae. Salivary glands contain more lysosomes than control salivary glands.

Expression of drprdsRNA.Scer\UAS under the control of either Scer\GAL4repo.PU or Scer\GAL4Mz709 completely suppresses the recruitment of ensheathing glia membranes to severed olfactory receptor neuron axons after severing of the maxillary palp.

Expression of drprdsRNA.Scer\UAS under the control of either Scer\GAL4repo.PU or Scer\GAL4Mz709 completely blocks glial clearance of axonal debris from the central nervous system after severing of the maxillary palp (axonal debris is efficiently cleared within 5 days after injury in wild-type controls). Expression of drprdsRNA.Scer\UAS under the control of Scer\GAL4alrm.PD has no effect on axon debris clearance after severing of the maxillary palp.

Glial-specific knock-down of drpr through expression of drprScer\UAS.dsRNA under the control of Scer\GAL4repo.PU fully suppresses glial responses to antennal or maxillary palp ablation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
NOT Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of drprdsRNA.Scer\UAS and Ced-12KK102788 under the control of Scer\GAL4alrm.PD does not increase the number of cell bodies or the amount of neurite debris when compared with elimination of each pathway alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
drprScer\UAS.dsRNA
drprdsRNA.Scer\UAS
drprdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (19)