FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
trbl, Akt
FlyBase ID
FBig0000109375
Interaction Network
Interactions Browser links
trbl network
Akt network
Reported Interactions
FBrf0226539-1.Y2H
Reference
Das et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbl 

trbl

bait

fused to GAL4 DNA-binding domain

trbl 
Akt 

Akt1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
kinase domain residue 264
mutation disrupting interaction
aa 264

D264N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Das et al., 2014)
FBrf0237536-1.coIP.WB
Reference
Fischer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbl 

Trbl

bait

FLAG tag

trbl 
trbl 
Akt 

Akt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
interaction domain residue 141
mutation decreasing interaction
aa 141

R141Q

trbl 
catalytic loop residue 264
mutation decreasing interaction
aa 264

D264N, referred to as D/NLK

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

(Fischer et al., 2017)
FBrf0237536-2.PD.A
Reference
Fischer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbl 

Trbl

bait

His tag

trbl 
Akt 

Akt

prey

fluorescent label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
interaction domain residue 141
mutation decreasing interaction
aa 141

R141Q

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Fischer et al., 2017)
FBrf0237536-3.Y2H
Reference
Fischer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbl 

Trbl

bait

fused to GAL4 DNA-binding domain

trbl 
trbl 
Akt 

Akt

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
interaction domain residue 141
mutation decreasing interaction
aa 141

R141Q

trbl 
interaction domain residue 141
mutation increasing interaction
aa 141

R141E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fischer et al., 2017)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Fischer et al., 2017)
FBrf0237536-5.coIP.WB
Reference
Fischer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
fat body / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbl 

Trbl

bait

FLAG tag

trbl 
Akt 

Akt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
interaction domain residue 141
mutation increasing interaction
aa 141

R141E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fat bodies of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fischer et al., 2017)
FBrf0262229-1.Y2H
Reference
Fischer et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
trbl 

trbl

bait

fused to GAL4 DNA-binding domain

trbl 
Akt 

Akt

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
trbl 
SLE motif residue 284
mutation increasing interaction
aa 284

S284G; coordinates relative to trbl-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Fischer et al., 2025)

Two-hybrid system: yeast GAL4-BD/GAL4-AD. Yeast transformed with trbl SLE/G in the bait and Akt in the prey grew on higher concentrations of the 3-AT His3 inhibitor than the wild-type trbl bait-Akt prey co-transformants.

(Fischer et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)