FlyBase Allele Report: Dmel\Akt[04226]

(Jin et al., 2020, Slade and Staveley, 2015, Inamdar et al., 2014, Liu et al., 2014, Natarajan et al., 2013, Dahlgaard et al., 2012, Kockel et al., 2010, Parrish et al., 2009, Branco et al., 2008, Martin-Pena et al., 2006, Chen et al., 2003, Stocker et al., 2002, Scanga et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

tracheal primordium

(Jin et al., 2001)

Additional Information

Statement

Reference

A lacZ reporter in this P-element insertion allowed the zygotic pattern of expression of Akt1 to be deduced. The reporter is expressed in the tracheal placodes in a pattern similar to that of trh and btl.

(Jin et al., 2001)

Marker for

Reflects expression of

Akt

(Jin et al., 2001)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Inamdar et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Chen et al., 2003)

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

ameliorates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

exacerbates frontotemporal dementia

modeled by Hsap\CHMP2B^Intron5.UAS

(West et al., 2018)

ameliorates cancer

modeled by Csk^Q156Stop, Ras85D^V12.UAS

(Hirabayashi et al., 2013)

ameliorates carcinoma

modeled by Csk^Q156Stop, InR^A1325D.UAS, Ras85D^V12.UAS

(Hirabayashi and Cagan, 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size (with Akt¹)

(Vereshchagina et al., 2008)

abnormal developmental rate

(Slade and Staveley, 2015)

abnormal locomotor behavior | adult stage | male (with Akt^MI14526)

(Jin et al., 2020)

abnormal locomotor behavior | larval stage (with Akt^MI14526)

(Jin et al., 2020)

abnormal neuroanatomy

(Martin-Pena et al., 2006)

abnormal neuroanatomy | third instar larval stage

(Natarajan et al., 2013)

abnormal neuroanatomy | third instar larval stage (with Df(3R)Exel7328)

(Natarajan et al., 2013)

abnormal neurophysiology | recessive | third instar larval stage

(Guo and Zhong, 2006)

abnormal neurophysiology | third instar larval stage (with Akt¹)

(Guo and Zhong, 2006)

decreased body size

(Gao et al., 2000)

decreased body size | recessive

(Guo and Zhong, 2006)

decreased cell size

(Gao et al., 2000)

female sterile | recessive

(Guo and Zhong, 2006)

lethal | embryonic stage | maternal effect

(Perrimon et al., 1996)

lethal | recessive

(Spradling et al., 1999, Perrimon et al., 1996)

partially lethal - majority die

partially lethal - majority die | recessive

(Guo and Zhong, 2006, Gao et al., 2000, Berkeley Drosophila Genome Project, 1993.6.1)

viable

(Slade and Staveley, 2015, Lee and Chung, 2007, Stocker et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Emergence of homozygous Akt1⁰⁴²²⁶ mutant flies is delayed by two to four days compared with controls.

The eyes of homozygous Akt1⁰⁴²²⁶ mutant flies exhibit a decrease in both ommatidial number and size compared with controls. The ommatidia in Akt1⁰⁴²²⁶ mutant eye clones are reduced in size compared to the homozygous flies.

(Slade and Staveley, 2015)

Akt1⁰⁴²²⁶ heterozygous mutants exhibit extreme sensitivity to 1-octen-3-ol. These flies survive for approximately 13 days when exposed to 0.5ppm 1-octen-3-ol, significantly lower than the 18 days-long survival span observed for exposed wild-type flies.

(Inamdar et al., 2014)

The neuromuscular junctions of homozygous Akt1⁰⁴²²⁶ mutant larvae (muscle 4 of segment A2-4) show an ~80% increase in synaptic growth compared to controls. These mutants also have an increase in brp positive active zones and a decrease in brp intensity.

The neuromuscular junctions of Akt1⁰⁴²²⁶/Df(3R)Exel7328 mutant larvae (muscle 4 of segment A2-4) show a significant increase in synaptic growth compared to controls. These mutants also have an increase in brp positive active zones.

(Natarajan et al., 2013)

No retinal phenotypes are seen in Akt1⁰⁴²²⁶ mutant flies.

(Branco et al., 2008)

Akt1⁰⁴²²⁶/Akt1¹ flies are about half the weight of control animals.

(Vereshchagina et al., 2008)

Long term synaptic depression at larval neuromuscular junctions is strongly impaired in Akt1⁰⁴²²⁶/Akt1¹ third instar larvae. This effect is seen when LTD is tested using 0.2 or 0.4 mM Ca²⁺ saline or when high frequency stimulation (40Hz) is used. The same phenotype is seen in Akt1⁰⁴²²⁶ homozygotes.

(Guo and Zhong, 2006)

Reduction of Akt1 activity decreases synapse number in both Akt1⁰⁴²²⁶ heterozygous and homozygous animals.

(Martin-Pena et al., 2006)

Germline clones produce eggs with poor cuticle development.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Akt¹/Akt⁰⁴²²⁶ has abnormal body size phenotype, suppressible by wdb[+]/wdb^IP

(Vereshchagina et al., 2008)

Enhancer of

Statement

Reference

Akt⁰⁴²²⁶ is an enhancer of visible | adult stage phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU

(West et al., 2018)

Akt1[+]/Akt⁰⁴²²⁶ is an enhancer of decreased cell size phenotype of Girdin^RNAi.UAS, Scer\GAL4^en-e16E

(Puseenam et al., 2009)

NOT Enhancer of

Statement

Reference

Akt1[+]/Akt⁰⁴²²⁶ is a non-enhancer of visible | adult stage phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Akt1[+]/Akt⁰⁴²²⁶ is a non-enhancer of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Suppressor of

Statement

Reference

Akt⁰⁴²²⁶ is a suppressor of decreased cell size phenotype of Cds^NIG.7962R, Scer\GAL4^αTub84B.PL

(Liu et al., 2014)

Akt1[+]/Akt⁰⁴²²⁶, Scer\GAL4^GMR.PU is a suppressor of visible phenotype of Elp6^UAS.cBa, Scer\GAL4^GMR.PU

(Bolukbasi et al., 2012)

Akt1[+]/Akt⁰⁴²²⁶ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of egh^62d18

(Dahlgaard et al., 2012)

Akt1[+]/Akt⁰⁴²²⁶ is a suppressor of visible | adult stage phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Akt⁰⁴²²⁶/Akt³ is a suppressor of lethal | recessive | larval stage phenotype of Pten^unspecified

(Stocker et al., 2002)

Akt⁰⁴²²⁶ is a suppressor of increased cell size | somatic clone phenotype of Pten^dj189

(Gao et al., 2000)

NOT Suppressor of

Statement

Reference

Akt1[+]/Akt⁰⁴²²⁶ is a non-suppressor of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Akt1[+]/Akt⁰⁴²²⁶ is a non-suppressor of increased cell number | somatic clone | cell non-autonomous phenotype of Uba1^A1

(Yan et al., 2009)

Akt⁰⁴²²⁶ is a non-suppressor of visible phenotype of Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

Akt⁰⁴²²⁶ is a non-suppressor of abnormal size | adult stage phenotype of Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

Akt⁰⁴²²⁶ is a non-suppressor of lethal | recessive | larval stage phenotype of Pten^unspecified

(Stocker et al., 2002)

Other

Statement

Reference

Akt1[+]/Akt⁰⁴²²⁶, gig¹⁰⁹ has abnormal neuroanatomy | third instar larval stage phenotype

(Natarajan et al., 2013)

Akt⁰⁴²²⁶, rictor^Δ24 has lethal phenotype

(Lee and Chung, 2007)

Akt⁰⁴²²⁶, rictor^Δ42 has lethal phenotype

(Lee and Chung, 2007)

Akt⁰⁴²²⁶/Akt³, Pten^unspecified has increased body size phenotype

(Stocker et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Akt⁰⁴²²⁶ has eye phenotype, enhanceable by foxo²⁵

(Slade and Staveley, 2015)

Akt⁰⁴²²⁶ has ommatidium phenotype, enhanceable by foxo²⁵

(Slade and Staveley, 2015)

Suppressed by

Statement

Reference

Akt⁰⁴²²⁶ has eye phenotype, suppressible by foxo²⁵

(Slade and Staveley, 2015)

Akt⁰⁴²²⁶ has ommatidium phenotype, suppressible | partially by foxo²⁵

(Slade and Staveley, 2015)

Enhancer of

Statement

Reference

Akt⁰⁴²²⁶ is an enhancer of eye phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU

(West et al., 2018)

Akt⁰⁴²²⁶ is an enhancer of eye phenotype of foxo²⁵

(Slade and Staveley, 2015)

Akt⁰⁴²²⁶ is an enhancer of ommatidium phenotype of foxo²⁵

(Slade and Staveley, 2015)

Akt1[+]/Akt⁰⁴²²⁶ is an enhancer of wing | posterior phenotype of Girdin^RNAi.UAS, Scer\GAL4^en-e16E

(Puseenam et al., 2009)

Akt1[+]/Akt⁰⁴²²⁶ is an enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

NOT Enhancer of

Statement

Reference

Akt1[+]/Akt⁰⁴²²⁶ is a non-enhancer of eye phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Padash Barmchi et al., 2016)

Akt1[+]/Akt⁰⁴²²⁶ is a non-enhancer of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Suppressor of

Statement

Reference

Akt⁰⁴²²⁶ is a suppressor of embryonic/larval fat body phenotype of Cds^NIG.7962R, Scer\GAL4^αTub84B.PL

(Liu et al., 2014)

Akt1[+]/Akt⁰⁴²²⁶, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Elp6^UAS.cBa, Scer\GAL4^GMR.PU

(Bolukbasi et al., 2012)

Akt1[+]/Akt⁰⁴²²⁶ is a suppressor of larval peripheral nervous system | third instar larval stage phenotype of egh^62d18

(Dahlgaard et al., 2012)

Akt1[+]/Akt⁰⁴²²⁶ is a suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Akt1[+]/Akt⁰⁴²²⁶ is a suppressor of ommatidium phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Akt1[+]/Akt⁰⁴²²⁶ is a suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Akt⁰⁴²²⁶ is a suppressor of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Akt⁰⁴²²⁶ is a suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Akt⁰⁴²²⁶ is a suppressor of rhabdomere phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Akt⁰⁴²²⁶ is a suppressor of wing margin bristle | somatic clone phenotype of Pten^dj189

(Gao et al., 2000)

NOT Suppressor of

Statement

Reference

Akt⁰⁴²²⁶ is a non-suppressor of eye phenotype of foxo²⁵

(Slade and Staveley, 2015)

Akt⁰⁴²²⁶ is a non-suppressor of ommatidium phenotype of foxo²⁵

(Slade and Staveley, 2015)

Akt1[+]/Akt⁰⁴²²⁶ is a non-suppressor of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

Akt1[+]/Akt⁰⁴²²⁶ is a non-suppressor of embryonic/larval plasmatocyte | ectopic phenotype of egh^62d18

(Dahlgaard et al., 2012)

Akt1[+]/Akt⁰⁴²²⁶ is a non-suppressor of eye disc | somatic clone | cell non-autonomous phenotype of Uba1^A1

(Yan et al., 2009)

Akt⁰⁴²²⁶ is a non-suppressor of eye phenotype of Mmus\Mdm2^UAS.cFa, Scer\GAL4^GMR.PF

(Folberg-Blum et al., 2002)

Other

Statement

Reference

Akt1[+]/Akt⁰⁴²²⁶, gig¹⁰⁹ has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Natarajan et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in ommatidium area seen in either mutant alone is enhanced in Akt1⁰⁴²²⁶ foxo²⁵ double mutant flies. As in the foxo²⁵ mutant alone, ommatidium number is increased.

(Slade and Staveley, 2015)

The rough eye phenotype observed in adults expressing Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is not modified by combination with single copy of Akt1⁰⁴²²⁶.

(Zaessinger et al., 2015)

A Akt1⁰⁴²²⁶ heterozygous background results in a reduction in the size of fat cells by around 50% in flies expressing CdsA^NIG.7962R under the control of Scer\GAL4^ppl.PP.

(Liu et al., 2014)

The neuromuscular junctions of Akt1⁰⁴²²⁶/+ gig¹⁰⁹/+ mutant larvae (muscle 4 of segment A2-4) exhibit increased synaptic growth in comparison with the single heterozygote controls.

(Natarajan et al., 2013)

The rough eye phenotype caused by expression of poly^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PU is suppressed by Akt1⁰⁴²²⁶/+.

(Bolukbasi et al., 2012)

Heterozygous Akt1⁰⁴²²⁶ strongly suppresses the egh^62d18 larval nerve phenotype regarding nerve diameter, but not the nerve-attached plasmatocyte-phenotype.

(Dahlgaard et al., 2012)

Akt1⁰⁴²²⁶/+ strongly enhances the Girdin^{dsRNA.Scer\UAS}, Scer\GAL4^en-e16E wing phenotype, with a dramatic reduction in cell size and posterior wing compartment area.

(Puseenam et al., 2009)

A Akt1⁰⁴²²⁶/+ background has no effect on the non-autonomous overgrowth phenotype found in Uba1^A1 mosaic eye clones.

(Yan et al., 2009)

Akt1⁰⁴²²⁶/Akt1¹ flies heterozygous for wdb^IP show a significant increase in body size and weight.

(Vereshchagina et al., 2008)

Xenogenetic Interactions

Statement

Reference

The eye degeneration phenotype characteristic for adult flies expressing Hsap\CHMP2B^Intron5.UAS under the control of Scer\GAL4^GMR.PU is strongly enhanced by combination with Akt1⁰⁴²²⁶.

(West et al., 2018)

Heterozygosity for Akt1⁰⁴²²⁶ does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6^{Scer\UAS.T:Hsap\MYC} and Hsap\UBE3A^Scer\UAS.cRa under the control of Scer\GAL4^GMR.PU, leading to severe eye necrosis.

(Padash Barmchi et al., 2016)

One copy of Akt1⁰⁴²²⁶ enhances the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

One copy of Akt1⁰⁴²²⁶ suppresses the external eye and retinal phenotypes seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

The eye phenotype caused by expression of Mmus\Mdm2^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PF is not rescued if the flies are also carrying either Akt1⁰⁴²²⁶.

(Folberg-Blum et al., 2002)

Complementation and Rescue Data

Fails to complement

Akt¹

(Scanga et al., 2000)

Partially rescued by

Akt⁰⁴²²⁶ is partially rescued by Scer\GAL4^arm.PU/Akt^UAS.cSa

(Slade and Staveley, 2015)

Comments

Expression of Akt1^Scer\UAS.cSa under the control of Scer\GAL4^arm.PU partially rescues the reductions in ommatidial size and number seen in homozygous Akt1⁰⁴²²⁶ mutant flies.

(Slade and Staveley, 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11627

ry⁵⁰⁶ P{PZ}Akt⁰⁴²²⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(3)05203⁰⁰⁹³⁴. Complements: srp⁰¹⁵⁴⁹. Complements: l(3)05203⁰⁸²³⁵. Complements: l(3)j1A1^j1A1. Complements: srp^neo45. Complements: l(3)neo46¹. Complements: l(3)neo47¹.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

AKT⁰⁴²²⁶

(West et al., 2018)

Akt1⁰⁴²²⁶

(Garcia et al., 2024, Ribot et al., 2022, Jin et al., 2020, Sato and Shibuya, 2018, Lin et al., 2016, Slade and Staveley, 2015, Inamdar et al., 2014, Natarajan et al., 2013, Bolukbasi et al., 2012, Dahlgaard et al., 2012, Schoenherr et al., 2012, Tokusumi et al., 2012, Vereshchagina et al., 2008, Martin-Pena et al., 2006, Folberg-Blum et al., 2002)

Akt1^P1627

Akt⁰⁴²²⁶

(Banzai and Nishimura, 2023, Padash Barmchi et al., 2016, Dahlgaard et al., 2012, Nechipurenko and Broihier, 2012, Zheng and Sehgal, 2010, Puseenam et al., 2009, Lee and Chung, 2007)

Akt^P1627

(Jin et al., 2001)

Dakt^l(3)04226

(Gao et al., 2000)

akt1⁰⁴²²⁶

(Deichsel et al., 2024, Deichsel et al., 2024)

akt⁰⁴²²⁶

(Datta et al., 2023, Hirabayashi and Cagan, 2015, Hirabayashi et al., 2013)

akt⁴²²⁶

(Guo and Zhong, 2006)

akt^hypo

(Hirabayashi et al., 2013)

dAkt1⁰⁴²²⁶

(Chen et al., 2003)

dAkt⁴²²⁶

(Stocker et al., 2002)

dPKB²

(Oldham and Hafen, 2003)

l(3)04226

(Scanga et al., 2000, Spradling et al., 1999)

l(3)04226⁰⁴²²⁶

(Perrimon et al., 1996, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project, 1993.6.1)

Name Synonyms

Secondary FlyBase IDs

FBal0009507
FBal0150982

References (45)

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