FlyBase Allele Report: Hsap\CHMP2B[Intron5.UAS]

General Information

Symbol

Hsap\CHMP2B^Intron5.UAS

Species

H. sapiens

Name

FlyBase ID

FBal0241178

Feature type

allele

Associated gene

Hsap\CHMP2B

Associated Insertion(s)

Carried in Construct

P{UAS-CHMP2B.ΔC}

Also Known As

UAS-CHMP2B^Intron5, CHMP2B^Intron5

Key Links

Transgenic product class

inframe_deletion

(Ahmad et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Ahmad et al., 2009)

Mutagen

in vitro construct

(Ahmad et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-CHMP2B.ΔC}

Cytology

Description

UAS regulatory sequences drive expression of Hsap\CHMP2B coding sequences in which the final 35 amino acid at the C-terminal are removed.

(Ahmad et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\CHMP2B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of frontotemporal dementia

CEA

(Yuva-Aydemir et al., 2019, West et al., 2018, West et al., 2015)

model of frontotemporal dementia and/or amyotrophic lateral sclerosis 7

CEC

(Jun et al., 2023, Lee et al., 2019)

CEA

(Chen et al., 2022, Lu et al., 2020, Wilson et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of frontotemporal dementia

is exacerbated by Rab8¹

(West et al., 2015)

is ameliorated by Rab8^UAS.cWa

(West et al., 2015)

is exacerbated by Rab8^RNAi.UAS

(West et al., 2015)

is exacerbated by Akt^HMS00007

(West et al., 2018)

is exacerbated by Akt⁰⁴²²⁶

(West et al., 2018)

is exacerbated by Akt³

(West et al., 2018)

is ameliorated by POSH⁷⁴

(West et al., 2018)

is ameliorated by POSH^HMC05588

(West et al., 2018)

is exacerbated by Akt^{ΔPH.UAS.Tag:Myr(Unk)}

(West et al., 2018)

model of frontotemporal dementia and/or amyotrophic lateral sclerosis 7

is ameliorated by zip¹

(Jun et al., 2023)

is ameliorated by zip^GD1566

(Jun et al., 2023)

is ameliorated by zip^GL00623

(Jun et al., 2023)

is exacerbated by spas^HMC03560

(Chen et al., 2022)

is exacerbated by spas^JF02724

(Chen et al., 2022)

is ameliorated by N^l1N-ts1

(Wilson et al., 2020)

is exacerbated by IKKε^GL00160

(Lu et al., 2020)

is ameliorated by IKKε^{UAS.ORF.Tag:HA}

(Lu et al., 2020)

is exacerbated by IKKε^alice

(Lu et al., 2020)

is exacerbated by TER94^k15502

(Lu et al., 2020)

is exacerbated by ctp^GD4688

(Lu et al., 2020)

is exacerbated by ctp^KK115493

(Lu et al., 2020)

is exacerbated by hook¹¹

(Lu et al., 2020)

is exacerbated by hook⁷

(Lu et al., 2020)

is exacerbated by hook^GD12598

(Lu et al., 2020)

is exacerbated by spn-F²

(Lu et al., 2020)

is exacerbated by spn-F^HMC06087

(Lu et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Ahmad et al., 2009)

CHMP2B:p.Met178_Asp213delinsVal

(FlyBase curators, 2019-)

Variants Synonym(s)

CHMP2B:p.Met137_Asp172delinsVal

CHMP2B, IVS5AS, G-C

CHMP2B[Intron5]

CHMP2B:c.532-1G>C

Associated human disease model(s)

frontotemporal dementia and/or amyotrophic lateral sclerosis 7

External database links

ClinVar: 98003

Comments concerning this variant

In human, mutation in splice acceptor site results in truncation of 35 aa at the carboxyl terminus of the protein (pubmed:16041373).

G to C substitution in the acceptor splice site of exon 6; the last 36 amino acids are replaced with a single valine residue from the fifth intron (Krasniak and Ahmed, 2016; pubmed:26972529).

(FlyBase curators, 2019-)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^nSyb.PS

(West et al., 2018)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^nSyb.PS

(West et al., 2015)

increased cell death, with Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

increased cell death | third instar larval stage, with Scer\GAL4^nSyb.PS

(West et al., 2018)

lethal, with Scer\GAL4^elav-C155

(Ahmad et al., 2009)

lethal - all die before end of P-stage, with Scer\GAL4^nSyb.PS

(West et al., 2018)

melanotic necrosis | adult stage, with Scer\GAL4^GMR.PF

(Jun et al., 2023)

visible, with Scer\GAL4^GMR.PF

(West et al., 2015)

visible, with Scer\GAL4^GMR.PU

(Lu et al., 2013)

visible | adult stage, with Scer\GAL4^GMR.PF

(Jun et al., 2023)

visible | adult stage, with Scer\GAL4^GMR.PU

(West et al., 2018)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Jun et al., 2023, West et al., 2015, Ahmad et al., 2009)

eye, with Scer\GAL4^GMR.PU

(West et al., 2018, Lu et al., 2013)

larval brain | third instar larval stage, with Scer\GAL4^nSyb.PS

(West et al., 2018)

neuromuscular junction | third instar larval stage, with Scer\GAL4^nSyb.PS

(West et al., 2018)

NMJ bouton | third instar larval stage, with Scer\GAL4^nSyb.PS

(West et al., 2018, West et al., 2015)

photoreceptor, with Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

synapse | third instar larval stage, with Scer\GAL4^nSyb.PS

(West et al., 2015)

Detailed Description

Statement

Reference

Expression of Hsap\CHMP2B^Intron5.UAS under the control of Scer\GAL4^GMR.PU results in eye degeneration phenotype and expression driven by Scer\GAL4^nSyb.PS leads to synaptic overgrowth at neuromuscular junction (increased bouton number and NMJ length), aberrant brain accumulations of POSH protein, increased cell death in larval brain as well as reduced crawling velocity in third instar larvae.

(West et al., 2018)

Expression of Hsap\CHMP2B^{Intron5.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF leads to a mild phenotype characterized by melanotic deposits.

Expression of Hsap\CHMP2B^{Intron5.Scer\UAS} in motor neurons under the control of Scer\GAL4^nSyb.PS induces a 30% increase in synaptic growth compared with controls.

(West et al., 2015)

Expression of Hsap\CHMP2B^{Intron5.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in a few small melanin deposits (indicating degeneration) in the eye in most flies.

(Lu et al., 2013)

Pan-neural expression of Hsap\CHMP2B^{Intron5.Scer\UAS} results in lethality.

Expression of Hsap\CHMP2B^{Intron5.Scer\UAS} in the eye, under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype and the appearance of black spots. Internal eye structure is also disrupted.

(Ahmad et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Akt^HMS00007, Scer\GAL4^GMR.PU

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Akt⁰⁴²²⁶

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Akt³

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Akt¹

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Df(3L)ED228/+

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Rab8¹

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by Rab8^RNAi.UAS, Scer\GAL4^GMR.PF

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Df(3R)ED5623/+

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Df(3R)ED5642/+

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Nsf2^A6/Nsf2[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Nsf2^A15/Nsf2[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Nsf2^GD1327, Scer\GAL4^GMR.PU

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by alphaSnap[+]/αSnap^M4

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by alphaSnap[+]/αSnap^G8

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Syx13⁰¹⁴⁷⁰/Syx13[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Syx13^RNAi.UAS.cUa, Scer\GAL4^GMR.PU

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Syx17^f01971/Syx17[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, enhanceable by Syx17^RNAi.cUa, Scer\GAL4^GMR.PU

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Mmus\Chmp4b^UAS.cAa, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by CG9779[+]/Vps24^EY04708, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Vps28[+]/Vps28^k16503, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by TSG101[+]/TSG101^f00976, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Df(3L)ED210/+, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by +/Df(2L)ED1243, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Df(3R)ED5664/+, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Spn88Ea^c01214, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Spn88Ea^GS9853, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by cact[+]/cact¹, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by cact⁴/cact[+], Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by cact[+]/cact^KG07677, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Spn88Ea^c01214/Spn5[+], Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by shrb[+]/shrb⁴, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, enhanceable by Vps2^GS11024/vps2[+], Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

NOT Enhanced by

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-enhanceable by Df(3R)ED5612/+

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible phenotype, non-enhanceable by Syx18^EY08095/Syx18[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, non-enhanceable by Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Suppressed by

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible by zip^GL00623, Scer\GAL4^GMR.PF

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has melanotic necrosis | adult stage phenotype, suppressible by zip^GL00623, Scer\GAL4^GMR.PF

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible | partially by zip^GD1566, Scer\GAL4^GMR.PF

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has melanotic necrosis | adult stage phenotype, suppressible | partially by zip^GD1566, Scer\GAL4^GMR.PF

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has visible | adult stage phenotype, suppressible | partially by zip¹/zip¹

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has melanotic necrosis | adult stage phenotype, suppressible | partially by zip¹/zip¹

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Akt^UAS.ORF, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by POSH^HMC05588, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by POSH⁷⁴/POSH⁷⁴

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has lethal - all die before end of P-stage phenotype, suppressible by POSH⁷⁴/POSH⁷⁴

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Akt^{ΔPH.UAS.Tag:Myr(Unk)}, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by Df(3L)H99

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF, Spn88Ea^c01214/Spn5[+] has increased cell death phenotype, suppressible by Spn88Ea^UAS.cAa, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

NOT suppressed by

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has increased cell death phenotype, non-suppressible by Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Enhancer of

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Mmus\Chmp4b^UAS.cAa, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Akt^HMS00007, Scer\GAL4^GMR.PU

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Akt⁰⁴²²⁶

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Akt³

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Akt¹

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Df(3L)ED228/+

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Rab8¹

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Rab8^RNAi.UAS, Scer\GAL4^GMR.PF

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Df(3R)ED5623/+

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Df(3R)ED5642/+

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Nsf2^A6/Nsf2[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Nsf2^A15/Nsf2[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Nsf2^GD1327, Scer\GAL4^GMR.PU

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by alphaSnap[+]/αSnap^M4

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by alphaSnap[+]/αSnap^G8

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Syx13⁰¹⁴⁷⁰/Syx13[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Syx13^RNAi.UAS.cUa, Scer\GAL4^GMR.PU

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Syx17^f01971/Syx17[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Syx17^RNAi.cUa, Scer\GAL4^GMR.PU

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by CG9779[+]/Vps24^EY04708, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Vps28[+]/Vps28^k16503, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by TSG101[+]/TSG101^f00976, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Df(3L)ED210/+, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by +/Df(2L)ED1243, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Df(3R)ED5664/+, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Spn88Ea^c01214, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Spn88Ea^GS9853, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by cact[+]/cact¹, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by cact⁴/cact[+], Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Mmus\Chmp4b^UAS.cAa, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by cact[+]/cact^KG07677, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Spn88Ea^c01214/Spn5[+], Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by shrb[+]/shrb⁴, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Vps2^GS11024/vps2[+], Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

NOT Enhanced by

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Df(3R)ED5612/+

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Syx18^EY08095/Syx18[+]

(Lu et al., 2013)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Suppressed by

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by zip^GL00623, Scer\GAL4^GMR.PF

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by zip^GD1566, Scer\GAL4^GMR.PF

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by zip¹/zip¹

(Jun et al., 2023)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has neuromuscular junction | third instar larval stage phenotype, suppressible by Akt^UAS.ORF, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has NMJ bouton | third instar larval stage phenotype, suppressible by Akt^UAS.ORF, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has neuromuscular junction | third instar larval stage phenotype, suppressible by POSH^HMC05588, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has NMJ bouton | third instar larval stage phenotype, suppressible by POSH^HMC05588, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has neuromuscular junction | third instar larval stage phenotype, suppressible by POSH⁷⁴/POSH⁷⁴

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has NMJ bouton | third instar larval stage phenotype, suppressible by POSH⁷⁴/POSH⁷⁴

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has neuromuscular junction | third instar larval stage phenotype, suppressible by Akt^{ΔPH.UAS.Tag:Myr(Unk)}, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has NMJ bouton | third instar larval stage phenotype, suppressible by Akt^{ΔPH.UAS.Tag:Myr(Unk)}, Scer\GAL4^nSyb.PS

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Df(3L)H99

(West et al., 2018)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has NMJ bouton | third instar larval stage phenotype, suppressible by Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^nSyb.PS has synapse | third instar larval stage phenotype, suppressible by Rab8^UAS.cWa, Scer\GAL4^nSyb.PS

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible | partially by Rab8^UAS.cWa, Scer\GAL4^GMR.PF

(West et al., 2015)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by PPO1^Bc/Bc[+], Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Spn88Ea^UAS.cAa, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF, Spn88Ea^c01214/Spn5[+] has eye phenotype, suppressible by Spn88Ea^UAS.cAa, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

NOT suppressed by

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Mmus\Cd8a^UAS.cLa.EGFP, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF has photoreceptor phenotype, non-suppressible by Spn88Ea^UAS.cAa, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Enhancer of

Statement

Reference

Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Mmus\Chmp4b^UAS.cAa, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The eye degeneration phenotype characteristic for adult flies expressing Hsap\CHMP2B^Intron5.UAS under the control of Scer\GAL4^GMR.PU is strongly enhanced by co-expression of Akt1^HMS00007 or by combination with any of the following: Akt1⁰⁴²²⁶, Akt1³, Akt1¹ or Df(3L)H99.

The neuromuscular junction synaptic overgrowth and larval locomotion deficits along with the abnormal brain accumulations of POSH protein induced by Scer\GAL4^nSyb.PS-driven expression of Hsap\CHMP2B^Intron5.UAS is ameliorated by co-expression of Akt1^UAS.ORF, Akt1^{ΔPH.UAS.Tag:Myr(Unk)}, POSH^HMC05588 or by POSH⁷⁴ homozygosity, which also decreases the elevated cell death in the larval brain.

(West et al., 2018)

The synaptic overgrowth phenotype resulting from the expression of Scer\GAL4^nSyb.PS>Hsap\CHMP2B^{Intron5.Scer\UAS} is suppressed when Rab8^Scer\UAS.cWa is co-expressed.

(West et al., 2015)

Expression of Mmus\Chmp4b with Hsap\CHMP2B^{Intron5.Scer\UAS} greatly enhances the severity of the rough eye phenotype compared to both single mutants (both under the control of Scer\GAL4^GMR.PF).

Expression of Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} with Hsap\CHMP2B^{Intron5.Scer\UAS} does not affect the severity of the rough eye phenotype (both under the control of Scer\GAL4^GMR.PF).

A single copy of shrb⁴, vps2^GS11024, vps24^EY04708, Vps28^k16503, TSG101^f00976 or Df(3L)ED210 significantly enhances the Hsap\CHMP2B^{Intron5.Scer\UAS} Scer\GAL4^GMR.PF phenotype in 1-day-old flies.

The Hsap\CHMP2B^{Intron5.Scer\UAS} Scer\GAL4^GMR.PF eye phenotype is significantly enhanced by a Df(2L)ED1243/+ or Df(3R)ED5664/+ background.

Spn5^c01214 significantly enhances the Hsap\CHMP2B^{Intron5.Scer\UAS} Scer\GAL4^GMR.PF eye phenotype.

Spn5^GS9853 significantly enhances the Hsap\CHMP2B^{Intron5.Scer\UAS} Scer\GAL4^GMR.PF eye phenotype.

The Scer\GAL4^GMR.PF Hsap\CHMP2B^{Intron5.Scer\UAS}-mediated eye black spots are suppressed in a Bc¹/+ background.

The presence of one copy of spz² or spz^KG05402 markedly reduces the number of flies with melanin deposits caused by expression of Hsap\CHMP2B^{Intron5.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

The presence of a copy of different cact mutant alleles, cact¹, cact⁴, or cact^KG07677 significantly enhanced the Hsap\CHMP2B^{Intron5.Scer\UAS} Scer\GAL4^GMR.PF phenotype.

Expression of Spn5^Scer\UAS.cAa in the eye under the control of Scer\GAL4^GMR.PF suppresses the external eye phenotype caused by Hsap\CHMP2B^{Intron5.Scer\UAS} expression. However, Spn5^Scer\UAS.cAa overexpression does not rescue the photoreceptor degeneration phenotype.

Expression of Spn5^{Δsig.Scer\UAS} in the eye under the control of Scer\GAL4^GMR.PF fails to suppress the melanization phenotype caused by Hsap\CHMP2B^{Intron5.Scer\UAS} expression.

Expression of Spn5^Scer\UAS.cAa in the eye under the control of Scer\GAL4^GMR.PF completely suppresses the enhancement effect of Spn5^c01214/+ on the Hsap\CHMP2B^{Intron5.Scer\UAS} locus.

The presence of one copy of spz² markedly reduces the number of flies with melanin deposits caused by expression of Hsap\CHMP2B^{Intron5.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

The presence of one copy of spz^KG05402 markedly reduces the number of flies with melanin deposits caused by expression of Hsap\CHMP2B^{Intron5.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Ahmad et al., 2009)

Complementation and Rescue Data

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Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CHMP2B^Intron5

(West et al., 2018)

Hsap\CHMP2B^{Intron5.Scer\UAS}

Hsap\CHMP2B^Intron5.UAS

Name Synonyms

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References (14)

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