FlyBase Allele Report: Dmel\Vps28[k16503]

(Saja et al., 2010, Ahmad et al., 2009, Geng and MacDonald, 2007, Sevrioukov et al., 2005, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | embryonic stage | germline clone

(Sevrioukov et al., 2005)

lethal | embryonic stage | germline clone

(Sevrioukov et al., 2005)

lethal | first instar larval stage

(Sevrioukov et al., 2005)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

male sterile, with Scer\GAL4^arm.PS, Vps28^UAS.cSa

(Sevrioukov et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

A single copy of Vps28^k16503 does not cause an eye phenotype.

(Ahmad et al., 2009)

Fat-body cells mutant for Vps28^k16503 show an accumulation of autophagosomes in fed conditions. These cells show a reduction in autolysosomes in response to 4-hour starvation conditions.

(Juhász et al., 2008)

Flies with eyes carrying Vps28^k16503 clones exhibit a disorganization of the ommatidia that is externally visible as roughness of the compound eye. The majority of ommatidia within these eyes contain no visible defects, while some ommatidia are missing photoreceptor cells, some contain extra cells and others contain a full complement of cells but are still not oriented correctly. Cells within the retina contain abnormally large multivesicular bodies that have internal vesicles.

Embryos produced from homozygous Vps28^k16503 germ cells exhibit major defects early in development. About one-half of these eggs are not fertilized although the micropyles are not malformed. Embryos that are fertilized and undergo nuclear divisions show developmental arrest before complete cellularization. These embryos frequently show irregular distribution of nuclei and have no pole cells. Cells that do form display an aberrant droplet-like shape. The embryos exhibit defects in the actin cytoskeleton, with some regions being devoid of actin and others containing enlarged actin bundles.

Vps28^k16503 males that express Vps28^Scer\UAS.cSa under the control of Scer\GAL4^arm.PS are infertile and possess very few motile sperm. These males show a defect at sperm individualization, where the appearance of actin cones is disorganised compared to wild-type males.

(Sevrioukov et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Vps28[+]/Vps28^k16503, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

NOT Enhancer of

Statement

Reference

Vps28[+]/Vps28^k16503 is a non-enhancer of abnormal neuroanatomy phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Vps28^k16503 is a non-enhancer of abnormal eye color | somatic clone phenotype of or^49h

(Sevrioukov et al., 2005)

NOT Suppressor of

Statement

Reference

Vps28[+]/Vps28^k16503 is a non-suppressor of abnormal neuroanatomy phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Vps28^k16503 has ommatidium | somatic clone phenotype, non-enhanceable by or^49h

(Sevrioukov et al., 2005)

Enhancer of

Statement

Reference

Vps28[+]/Vps28^k16503, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\CHMP2B^Intron5.UAS, Scer\GAL4^GMR.PF

(Ahmad et al., 2009)

NOT Enhancer of

Statement

Reference

Vps28[+]/Vps28^k16503 is a non-enhancer of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Vps28^k16503 is a non-enhancer of pigment cell | somatic clone phenotype of or^49h

(Sevrioukov et al., 2005)

NOT Suppressor of

Statement

Reference

Vps28[+]/Vps28^k16503 is a non-suppressor of eye phenotype of Ppt1^UAS.cKa, Scer\GAL4^GMR.PF

(Saja et al., 2010)

Other

Statement

Reference

Pi3K59F^Δm22, Vps28^k16503 has embryonic/larval fat body | somatic clone phenotype

(Juhász et al., 2008)

Pi3K59F^Δm22, Vps28^k16503 has autophagosome | somatic clone phenotype

(Juhász et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

A Vps28^k16503 background does not enhance or suppress the eye degeneration phenotype found upon expression of Ppt1^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF.

(Saja et al., 2010)

Vps28^k16503 and Pi3K59F^Δm22 double mutant clones generated in the fat-body show accumulation of autophagosomes under fed conditions, compared to none observed in Pi3K59F^Δm22 single mutant clones.

(Juhász et al., 2008)

Eyes that carry Vps28^k16503, or^49h double mutant clones show the same two phenotypes as Vps28^k16503 and or^49h single mutant clones.

(Sevrioukov et al., 2005)

Xenogenetic Interactions

Statement

Reference

A single copy of Vps28^k16503 significantly enhances the Hsap\CHMP2B^{Intron5.Scer\UAS} Scer\GAL4^GMR.PF phenotype in 1-day-old flies.

(Ahmad et al., 2009)

Complementation and Rescue Data

Fails to complement

Vps28^k16519

(BDGP Project Members, 1994-1999)