FlyBase Allele Report: Dmel\foxo[25]

General Information

Symbol

Dmel\foxo²⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0151929

Feature type

allele

Associated gene

Dmel\foxo

Associated Insertion(s)

P{EP}EP35-147

Carried in Construct

Also Known As

dFOXO²⁵

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Dionne et al., 2006)

Mutagen

P-element activity

(Junger et al., 2003)

Progenitor genotype

foxo^EP35-147

(Junger et al., 2003)

Associated Insertion(s)

P{EP}EP35-147

Cytology

Description

The progenitor P{EP}EP35-147 insertion is also still present on the chromosome.

(Junger et al., 2003)

Amino acid replacement: W124term.

(Junger et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:14,067,088..14,067,088 [+]

Nucleotide change:

G14067088A

Amino acid change:

Reported amino acid change:

W124term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Junger et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Hong et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm (with foxo²¹)

(Zheng et al., 2007)

abnormal developmental rate (with foxo^Δ94)

(Slack et al., 2011)

abnormal developmental rate | nutrition conditional (with foxo²¹)

(Kramer et al., 2008)

abnormal immune response (with foxo^BG01018)

(Dionne et al., 2006)

abnormal locomotor behavior | adult stage | progressive (with foxo²¹)

(Koh et al., 2012)

abnormal oxidative stress response (with foxo²¹)

(Zheng et al., 2007)

abnormal stress response

(Junger et al., 2003)

abnormal stress response (with foxo²¹)

(Tettweiler et al., 2005)

decreased body size (with foxo²¹)

(Kramer et al., 2008)

decreased body size (with foxo^Δ94)

(Slack et al., 2011)

decreased cell number | adult stage (with foxo²¹)

(Koh et al., 2012)

increased speed of aging (with foxo²¹)

(Demontis and Perrimon, 2010)

short lived (with foxo²¹)

(Sun et al., 2012)

short lived | nutrition conditional (with foxo²¹)

(Kramer et al., 2008)

viable

(Liu and Lehmann, 2006, Junger et al., 2003)

Phenotype Manifest In

dorso-lateral dopaminergic neuron (with foxo²¹)

(Koh et al., 2012)

eye

(Slade and Staveley, 2015)

female germline stem cell | conditional (with foxo²¹)

(Xing et al., 2015)

indirect flight muscle cell | adult stage | progressive (with foxo²¹)

(Koh et al., 2012)

muscle cell (with foxo²¹)

(Demontis and Perrimon, 2010)

neuroblast MBp

(Siegrist et al., 2010)

ommatidium

(Slade and Staveley, 2015)

testis (with foxo²¹)

(Xing et al., 2015)

wing

(Junger et al., 2003)

wing (with foxo^Δ94)

(Slack et al., 2011)

Detailed Description

Statement

Reference

The eyes of foxo²⁵ mutant flies have a smaller average ommatidia area than controls and a higher ommatidia number.

(Slade and Staveley, 2015)

foxo²¹/foxo²⁵ mutant testes contain significantly more undifferentiated germ cells compared to heterozygous controls.

foxo²¹/foxo²⁵ mutant germaria show increased germline stem cell loss following irradiation compared with controls.

(Xing et al., 2015)

Mutant embryos have a normal somatic muscle pattern. The size of the segmental border muscle and the DA1 muscle and also the number of nuclei present in these muscles are normal.

(Tixier et al., 2013)

Adult brains have a significant decrease in dopaminergic neuron number (especially in the DL1 cluster) in 30 day old foxo²¹/foxo²⁵ flies. Climbing ability in foxo²¹/foxo²⁵ is significantly worse (at both 3 and even more so at 15 days old) and there is a significant reduction in ATP of the indirect flight muscle compared to wild type at 15 (but not 3) days old.

(Koh et al., 2012)

foxo²¹/foxo²⁵ flies are short lived relative to wild-type controls, but their lifespan patterns responding to diet are similar to their wild-type controls.

(Sun et al., 2012)

foxo²¹/foxo²⁵ mutant animals display accelerated muscle aging manifesting in the increased accumulation of age-related protein aggregates compared with wild-type flies.

(Demontis and Perrimon, 2010)

Mushroom body neuroblasts persist slightly longer than normal in mutant animals.

(Siegrist et al., 2010)

foxo²¹/foxo²⁵ transheterozygous larvae exhibit reduced survival and shorter life span under amino acid starvation conditions. These flies are also developmentally delayed under these conditions and are smaller than wild-type after 48 hours.

(Kramer et al., 2008)

foxo²⁵/foxo²¹ trans-heterozygotes exhibit normal lipid levels and are as resistant to starvation as wild-type flies.

(Wang et al., 2008)

Starvation-induced autophagy is strongly reduced compared to controls in foxo²¹/foxo²⁵ larvae.

(Juhász et al., 2007)

foxo²¹/foxo²⁵ mutants rapidly become arrythmic in the presence of low levels of paraquat (0.5-1mM), while wild-type flies retain their rhythms for a few weeks. Phototaxis is not affected by paraquat treatment in these mutants.

foxo²¹/foxo²⁵ mutant flies are hypersensitive to oxidative stress.

30 day old foxo²¹/foxo²⁵ mutant flies show weak rhythms simliar to those seen in response to paraquat in young flies.

(Zheng et al., 2007)

foxo^BG01018/foxo²⁵ flies display a 4.8% improvement in medial survival following M.marinum infection in comparison to wild-type flies - 174 hours vs. 166 hours. foxo²¹/foxo²⁵ and foxo²⁵/Df(3R)ED5634 transheterozygotes survive even longer - 190 hours following infection. The number of bacteria recorded in these flies is not significantly different from wild-type flies.

(Dionne et al., 2006)

Salivary glands are destroyed at the same time in homozygous pupae as in wild-type pupae.

(Liu and Lehmann, 2006)

foxo²¹/foxo²⁵ flies exhibit a reduced life-span when exposed to 5% hydrogen peroxide-containing media (median life-span of 34.5 hours). Only 2% of foxo²¹/foxo²⁵ flies survived 60 hours after exposure to oxidative stress, compared to 66.1% in wild-type.

(Tettweiler et al., 2005)

Shows no obvious phenotype under normal culturing conditions, though close inspection reveals the wing size is slightly reduced. Clonal analysis in the head capsule reveals no effect on growth. Clonal analysis reveals no difference of cell size in the developing eye between mutant and wild type. No significant difference between body weight of mutant and wild type flies is detectable. When placed on hydrogen-peroxide-containing food mutant flies display significantly reduced survival time compared to control flies. A similar effect occurs in response to paraquat feeding.

(Junger et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

foxo²⁵/foxo²¹ has decreased cell number | adult stage phenotype, non-enhanceable by Pink1^B9

(Koh et al., 2012)

Suppressed by

Statement

Reference

foxo²⁵/foxo²¹ has abnormal stress response phenotype, suppressible by Scer\GAL4^Act5C.PU/Thor^UAS.cTa

(Tettweiler et al., 2005)

Enhancer of

Statement

Reference

foxo²⁵ is an enhancer of visible | somatic clone phenotype of Tsc1^Q87X

(Junger et al., 2003)

foxo²⁵/foxo²¹ is an enhancer of decreased cell size phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

NOT Enhancer of

Statement

Reference

foxo²⁵/foxo²¹ is a non-enhancer of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-enhancer of increased cell death | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo[+] is a non-enhancer of short lived phenotype of Crtc^25-3

(Wang et al., 2008)

foxo²⁵/foxo[+] is a non-enhancer of abnormal stress response phenotype of Crtc^25-3

(Wang et al., 2008)

Suppressor of

Statement

Reference

foxo²⁵/foxo²¹ is a suppressor of decreased cell number | oogenesis phenotype of InR^E19/InR³³⁹

(Su et al., 2018)

foxo²⁵/foxo²¹ is a suppressor of abnormal oxidative stress response | adult stage | chemical conditional phenotype of Trap1^EY21851

(Kim et al., 2016)

foxo²⁵/foxo[+] is a suppressor of increased cell death | somatic clone phenotype of pie^E1-16

(Xing et al., 2015)

foxo²⁵/foxo[+] is a suppressor of increased cell death | larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^elav.PU

(Hong et al., 2012)

foxo²⁵/foxo[+] is a suppressor of lethal phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^elav.PU

(Hong et al., 2012)

foxo²⁵/foxo[+] is a suppressor of abnormal locomotor behavior phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^elav.PU

(Hong et al., 2012)

foxo²⁵/foxo²⁵ is a suppressor of visible phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

foxo²⁵/foxo²⁵ is a suppressor | partially of abnormal eye color phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

foxo²⁵/foxo[+] is a suppressor | partially of decreased body size | adult stage phenotype of Scer\GAL4^Ilp2.PR, hep^Act.UAS

(Wang et al., 2005)

foxo²⁵/foxo[+] is a suppressor of long lived | dominant phenotype of puc^E69

(Wang et al., 2005)

foxo²⁵ is a suppressor | partially of visible | somatic clone phenotype of InR³⁰⁴

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of decreased cell number phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of visible phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor | partially of decreased body size phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵ is a suppressor | partially of visible | somatic clone phenotype of Pi3K92E^5W3

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of lethal | larval stage phenotype of Akt¹

(Junger et al., 2003)

foxo²⁵ is a suppressor of visible | somatic clone phenotype of Akt¹

(Junger et al., 2003)

foxo²⁵ is a suppressor of visible | somatic clone phenotype of Pten¹¹⁷

(Junger et al., 2003)

NOT Suppressor of

Statement

Reference

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of increased cell death | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo[+] is a non-suppressor of short lived phenotype of Crtc^25-3

(Wang et al., 2008)

foxo²⁵/foxo[+] is a non-suppressor of abnormal stress response phenotype of Crtc^25-3

(Wang et al., 2008)

foxo²⁵/foxo[+] is a non-suppressor of decreased cell death phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^hs.PB

(Liu and Lehmann, 2006)

foxo²⁵/foxo²⁵ is a non-suppressor of decreased cell death phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^hs.PB

(Liu and Lehmann, 2006)

Other

Statement

Reference

Crtc^25-3, foxo²⁵/foxo²¹ has lethal phenotype

(Wang et al., 2008)

Akt¹, foxo²⁵ has lethal phenotype

(Liu and Lehmann, 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

foxo²⁵ has eye phenotype, enhanceable by Akt⁵²

(Slade and Staveley, 2015)

foxo²⁵ has ommatidium phenotype, enhanceable by Akt⁵²

(Slade and Staveley, 2015)

foxo²⁵ has eye phenotype, enhanceable by Akt⁵⁷

(Slade and Staveley, 2015)

foxo²⁵ has ommatidium phenotype, enhanceable by Akt⁵⁷

(Slade and Staveley, 2015)

foxo²⁵ has eye phenotype, enhanceable by Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

foxo²⁵ has ommatidium phenotype, enhanceable by Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

NOT Enhanced by

Statement

Reference

foxo²⁵/foxo²¹ has dorso-lateral dopaminergic neuron phenotype, non-enhanceable by Pink1^B9

(Koh et al., 2012)

NOT suppressed by

Statement

Reference

foxo²⁵ has eye phenotype, non-suppressible by Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

foxo²⁵ has ommatidium phenotype, non-suppressible by Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

foxo²⁵ has eye phenotype, non-suppressible by Akt⁵²

(Slade and Staveley, 2015)

foxo²⁵ has ommatidium phenotype, non-suppressible by Akt⁵²

(Slade and Staveley, 2015)

foxo²⁵ has eye phenotype, non-suppressible by Akt⁵⁷

(Slade and Staveley, 2015)

foxo²⁵ has ommatidium phenotype, non-suppressible by Akt⁵⁷

(Slade and Staveley, 2015)

Enhancer of

Statement

Reference

foxo²⁵ is an enhancer of eye phenotype of Akt⁵²

(Slade and Staveley, 2015)

foxo²⁵ is an enhancer of ommatidium phenotype of Akt⁵²

(Slade and Staveley, 2015)

foxo²⁵ is an enhancer of eye phenotype of Akt⁵⁷

(Slade and Staveley, 2015)

foxo²⁵ is an enhancer of ommatidium phenotype of Akt⁵⁷

(Slade and Staveley, 2015)

foxo²⁵ is an enhancer of eye phenotype of Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

foxo²⁵ is an enhancer of ommatidium phenotype of Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

foxo²⁵ is an enhancer of adult head capsule | somatic clone phenotype of Tsc1^Q87X

(Junger et al., 2003)

foxo²⁵/foxo²¹ is an enhancer of ommatidium phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

NOT Enhancer of

Statement

Reference

foxo²⁵/foxo²¹ is a non-enhancer of adult thorax phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-enhancer of mitochondrion | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-enhancer of indirect flight muscle cell phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-enhancer of wing blade phenotype of Pink1^B9

(Koh et al., 2012)

Suppressor of

Statement

Reference

foxo²⁵/foxo²¹ is a suppressor of escort cell phenotype of InR^E19/InR³³⁹

(Su et al., 2018)

foxo²⁵ is a suppressor of eye phenotype of Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

foxo²⁵ is a suppressor | partially of ommatidium phenotype of Akt⁰⁴²²⁶

(Slade and Staveley, 2015)

foxo²⁵ is a suppressor of eye phenotype of Akt⁵²

(Slade and Staveley, 2015)

foxo²⁵ is a suppressor | partially of ommatidium phenotype of Akt⁵²

(Slade and Staveley, 2015)

foxo²⁵ is a suppressor of eye phenotype of Akt⁵⁷

(Slade and Staveley, 2015)

foxo²⁵ is a suppressor | partially of ommatidium phenotype of Akt⁵⁷

(Slade and Staveley, 2015)

foxo²⁵/foxo[+] is a suppressor of male germline stem cell | germline clone phenotype of pie^E1-16

(Xing et al., 2015)

foxo²⁵/foxo[+] is a suppressor of eye disc | somatic clone phenotype of pie^E1-16

(Xing et al., 2015)

foxo²⁵/foxo[+] is a suppressor of eye phenotype of Hsap\APP^{Aβ42.GMR.Tag:SS(rPENK)}

(Hong et al., 2012)

foxo²⁵/foxo²⁵ is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

foxo²⁵ is a suppressor | partially of adult head capsule | somatic clone phenotype of InR³⁰⁴

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of ommatidium phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵/foxo²¹ is a suppressor of wing phenotype of chico^flp147E/chico¹

(Junger et al., 2003)

foxo²⁵ is a suppressor | partially of adult head capsule | somatic clone phenotype of Pi3K92E^5W3

(Junger et al., 2003)

foxo²⁵ is a suppressor of adult head capsule | somatic clone phenotype of Akt¹

(Junger et al., 2003)

foxo²⁵ is a suppressor of adult head capsule | somatic clone phenotype of Pten¹¹⁷

(Junger et al., 2003)

NOT Suppressor of

Statement

Reference

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of wing blade phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of adult thorax phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵/foxo²¹ is a non-suppressor of eye phenotype of Crtc^UAS.cWa, Scer\GAL4^GMR.PF

(Wang et al., 2008)

foxo²⁵/foxo[+] is a non-suppressor of embryonic/larval salivary gland phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^hs.PB

(Liu and Lehmann, 2006)

foxo²⁵/foxo²⁵ is a non-suppressor of embryonic/larval salivary gland phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^hs.PB

(Liu and Lehmann, 2006)

Other

Statement

Reference

Scer\GAL4^wor.PA, foxo²⁵, grim^RNAi.RHG.UAS, hid^RNAi.RHG.UAS, rpr^RNAi.RHG.UAS has adult mushroom body phenotype

(Siegrist et al., 2010)

Scer\GAL4^wor.PA, foxo²⁵, grim^RNAi.RHG.UAS, hid^RNAi.RHG.UAS, rpr^RNAi.RHG.UAS has neuroblast | ectopic phenotype

(Siegrist et al., 2010)

Akt¹, foxo²⁵ has embryonic/larval salivary gland phenotype

(Liu and Lehmann, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number of escort cells observed in the germarium of InR^E19/InR³³⁹ transheterozygotes is suppressed by additional foxo²¹/foxo²⁵ transheterozygosity.

(Su et al., 2018)

Presence of foxo²⁵/foxo²¹ significantly suppresses increased survival in response to rotenone or paraquat in Trap1^EY21851/Trap1^EY21851 flies. Presence of foxo²⁵/foxo²¹ suppresses rescue via feeding of G-TTP of locomotor defects in Pink1^B9/Pink1^B9 flies.

(Kim et al., 2016)

The reduction in ommatidium area seen in either mutant alone is enhanced in Akt1⁰⁴²²⁶ foxo²⁵ double mutant flies. As in the foxo²⁵ mutant alone, ommatidium number is increased.

The reduction in ommatidium area seen in either mutant alone is enhanced in Akt1⁵⁷ foxo²⁵ double mutant flies. As in the foxo²⁵ mutant alone, ommatidium number is increased.

The reduction in ommatidium area seen in either mutant alone is enhanced in Akt1⁵² foxo²⁵ double mutant flies. As in the foxo²⁵ mutant alone, ommatidium number is increased.

(Slade and Staveley, 2015)

One copy of foxo²⁵ significantly suppresses the male germline stem cell (GSC) loss and reduced GSC division rate seen in pie^E1-16 mutant clones.

One copy of foxo²⁵ significantly suppresses the increased apoptosis seen in pie^E1-16 mutant eye disc clones, and clones are seen in adult eyes.

(Xing et al., 2015)

Expression of Sirt1^Scer\UAS.cGa driven by Scer\GAL4^arm.PU partially suppresses phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in (3 day old) Pink1^B9/Y flies. Presence of foxo²⁵/foxo²¹ almost completely nullifies the suppressive effect of Scer\GAL4^arm.PU>Sirt1^Scer\UAS.cGa on phenotypes in Pink1^B9/Y flies.

foxo²⁵/foxo²¹ does not significantly enhance phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in Pink1^B9/Y flies.

Pink1^B9/Y does not enhance dopaminergic neuron loss (in the DL1 cluster) in foxo²¹/foxo²⁵ flies.

(Koh et al., 2012)

Lipid levels are completely restored in Sik3⁴⁸ ; foxo²¹/foxo²⁵ double mutant flies.

(Wang et al., 2011)

Neuroblasts are detected in the mushroom body of 2 week old and even in 1 month old adults simultaneously co-expressing rpr^{miRNA.RHG.Scer\UAS}, W^{miRNA.RHG.Scer\UAS} and grim^{miRNA.RHG.Scer\UAS} (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4^wor.PA in a foxo²⁵ background (mushroom body neuroblasts are not seen in wild-type adults and are not seen at these late time points in adults expressing the P{UAS-RHG.miRNA} transgene under the control of Scer\GAL4^wor.PA in a wild-type background or in foxo²⁵ single mutant adults). Some of the 2 week old mutant mushroom body neuroblasts are large in size and generate many new progeny. There is a strong correlation between mushroom body neuroblast cell size and progeny number in 2 week old mutant adults, but not in younger mutant adults. In 1 month old mutant adults, some of the progeny of these neuroblasts show normal axon projections through the mushroom body pedunculus and some mistarget, bifurcating prematurely and projecting anterior to the pedunculus.

(Siegrist et al., 2010)

A significant recovery of eye pigmentation and structure is seen in flies expressing Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU in a foxo²⁵/foxo²⁵ background.

(Griswold et al., 2008)

A foxo²⁵ heterozygous background has no effect on survival or lipid accumulation in starved TORC^25-3 homozygotes.

Removal of both copies of foxo (foxo²⁵/foxo²¹) is lethal in TORC^25-3 homozygotes.

TORC^Scer\UAS.cWa over-expressing flies (driven by Scer\GAL4^GMR.PF) in which foxo is reduced or eliminated though a foxo²⁵/foxo²¹ background still exhibit a rough eye phenotype.

(Wang et al., 2008)

foxo²⁵ Akt1¹ double homozygotes show considerable lethality; most animals die before the pupal stage, with most of the remaining animals dying as pupae and only a few escapers eclosing as adults. 71% of pupae still contain intact salivary glands at 20 hours after puparium formation (this is approximately 6 hours after the glands are normally destroyed in wild-type animals).

(Liu and Lehmann, 2006)

Ectopic expression of Thor^Scer\UAS.cTa, under the control of Scer\GAL4^Act5C can completely suppress the sensitivity of foxo²¹/foxo²⁵ flies to oxidative stress (median life-span of 56.8 hours, 39.7% survival rate after 60 hours exposure to 5% hydrogen peroxide).

(Tettweiler et al., 2005)

The increase in average and maximum lifespan seen in puc^E69/+ adults is dominantly suppressed by foxo²⁵. The reduction in body weight seen in 1 day old hep^Act.Scer\UAS; Scer\GAL4^Ilp2.PR adults is partially supressed by foxo²⁵/+.

(Wang et al., 2005)

Xenogenetic Interactions

Statement

Reference

The ability of 0.5mM Psammaplysene A to suppress the eye degeneration phenotype caused by expression of Hsap\AR^Q52.Scer\UAS under the control of Scer\GAL4^GMR.PU (in the presence of dihydrotestosterone) is suppressed if the flies are also heterozygous for foxo²⁵.

(Mojsilovic-Petrovic et al., 2009)

Complementation and Rescue Data

Rescued by

foxo²⁵/foxo²¹ is rescued by foxo^UAS.cFa/Scer\GAL4^Lk6-DJ634

(Zheng et al., 2007)

foxo²⁵/foxo²¹ is rescued by foxo^Tag:V5

(Zheng et al., 2007)

Not rescued by

foxo²⁵/foxo²¹ is not rescued by foxo^UAS.cFa/Scer\GAL4^P2.4.Pdf

(Zheng et al., 2007)

foxo²⁵/foxo²¹ is not rescued by foxo^Tag:V5

(Zheng et al., 2007)

Comments

Expression of foxo^T:SV5\V5 rescues the circadian arrhythmia seen in foxo²¹/foxo²⁵ mutants. Phototaxis is not affected by paraquat treatment in these mutants.

Expression of foxo^Scer\UAS.cFa under the control of Scer\GAL4^Lk6-DJ634 rescues the circadian arrhythmia seen in foxo²¹/foxo²⁵ mutants. Phototaxis is not affected by paraquat treatment in these mutants.

(Zheng et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

80944

y¹ w^*; foxo²⁵/TM6B, Tb¹

98118

w^*; P{neoFRT}82B foxo²⁵/TM6B, Tb¹

80948

w^*; P{Switch1}106; foxo²⁵/TM3, Sb¹

80945

y¹ w^*; P{foxo.V5}2A1; foxo²⁵/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

(Demontis and Perrimon, 2010)

FOXO²⁵

(Kim et al., 2016, Galagovsky et al., 2014, Koh et al., 2012, Wang et al., 2011, Francis et al., 2010, Teleman et al., 2005)

Foxo²⁵

(Juhász et al., 2007)

dFOXO²⁵

(Liu et al., 2014, Hong et al., 2012, Tokusumi et al., 2012, Chung et al., 2010, Vihervaara and Puig, 2008, Liu and Lehmann, 2006, Puig and Tjian, 2005, Junger et al., 2003)

dFoxO²⁵

(Kanao et al., 2010)

dFoxo^Rw25

(Slaidina et al., 2009)

dfoxo25c

(Slack et al., 2010)

dfoxo²⁵

(Sun et al., 2012, Buch et al., 2008)

foxo25

(Zid et al., 2009)

foxo^25c

(Mensah et al., 2015)

foxo^W124X

(Slade and Staveley, 2015)

Name Synonyms

Secondary FlyBase IDs

References (47)

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