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General Information
Symbol
Dmel\foxo25
Species
D. melanogaster
Name
FlyBase ID
FBal0151929
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dFOXO25
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G14067088A

Amino acid change:

W124term | foxo-PB; W124term | foxo-PC; W124term | foxo-PF; W124term | foxo-PG; W124term | foxo-PH

Reported amino acid change:

W124term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The progenitor P{EP}EP35-147 insertion is also still present on the chromosome.

Amino acid replacement: W124term.

Insertion components
P{EP}EP35-147
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The eyes of foxo25 mutant flies have a smaller average ommatidia area than controls and a higher ommatidia number.

foxo21/foxo25 mutant testes contain significantly more undifferentiated germ cells compared to heterozygous controls.

foxo21/foxo25 mutant germaria show increased germline stem cell loss following irradiation compared with controls.

Mutant embryos have a normal somatic muscle pattern. The size of the segmental border muscle and the DA1 muscle and also the number of nuclei present in these muscles are normal.

Adult brains have a significant decrease in dopaminergic neuron number (especially in the DL1 cluster) in 30 day old foxo21/foxo25 flies. Climbing ability in foxo21/foxo25 is significantly worse (at both 3 and even more so at 15 days old) and there is a significant reduction in ATP of the indirect flight muscle compared to wild type at 15 (but not 3) days old.

foxo21/foxo25 flies are short lived relative to wild-type controls, but their lifespan patterns responding to diet are similar to their wild-type controls.

foxo21/foxo25 mutant animals display accelerated muscle aging manifesting in the increased accumulation of age-related protein aggregates compared with wild-type flies.

Mushroom body neuroblasts persist slightly longer than normal in mutant animals.

foxo21/foxo25 transheterozygous larvae exhibit reduced survival and shorter life span under amino acid starvation conditions. These flies are also developmentally delayed under these conditions and are smaller than wild-type after 48 hours.

foxo25/foxo21 trans-heterozygotes exhibit normal lipid levels and are as resistant to starvation as wild-type flies.

Starvation-induced autophagy is strongly reduced compared to controls in foxo21/foxo25 larvae.

foxo21/foxo25 mutants rapidly become arrythmic in the presence of low levels of paraquat (0.5-1mM), while wild-type flies retain their rhythms for a few weeks. Phototaxis is not affected by paraquat treatment in these mutants.

foxo21/foxo25 mutant flies are hypersensitive to oxidative stress.

30 day old foxo21/foxo25 mutant flies show weak rhythms simliar to those seen in response to paraquat in young flies.

foxoBG01018/foxo25 flies display a 4.8% improvement in medial survival following M.marinum infection in comparison to wild-type flies - 174 hours vs. 166 hours. foxo21/foxo25 and foxo25/Df(3R)ED5634 transheterozygotes survive even longer - 190 hours following infection. The number of bacteria recorded in these flies is not significantly different from wild-type flies.

Salivary glands are destroyed at the same time in homozygous pupae as in wild-type pupae.

foxo21/foxo25 flies exhibit a reduced life-span when exposed to 5% hydrogen peroxide-containing media (median life-span of 34.5 hours). Only 2% of foxo21/foxo25 flies survived 60 hours after exposure to oxidative stress, compared to 66.1% in wild-type.

Shows no obvious phenotype under normal culturing conditions, though close inspection reveals the wing size is slightly reduced. Clonal analysis in the head capsule reveals no effect on growth. Clonal analysis reveals no difference of cell size in the developing eye between mutant and wild type. No significant difference between body weight of mutant and wild type flies is detectable. When placed on hydrogen-peroxide-containing food mutant flies display significantly reduced survival time compared to control flies. A similar effect occurs in response to paraquat feeding.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference
Suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

foxo25/foxo[+] is a non-enhancer of short lived phenotype of Crtc25-3

foxo25/foxo[+] is a non-enhancer of stress response defective phenotype of Crtc25-3

Suppressor of
Statement
Reference

foxo25/foxo21 is a suppressor of oxidative stress response defective | adult stage | chemical conditional phenotype of Trap1EY21851

foxo25/foxo[+] is a suppressor of increased cell death | somatic clone phenotype of pieE1-16

foxo25/foxo[+] is a suppressor of long lived | dominant phenotype of pucE69

foxo25 is a suppressor | partially of visible | somatic clone phenotype of Pten117

foxo25 is a suppressor | partially of visible | somatic clone phenotype of InR304

foxo25/foxo21 is a suppressor | partially of lethal | larval stage phenotype of Akt11

foxo25 is a suppressor | partially of visible | somatic clone phenotype of Akt11

foxo25/foxo21 is a suppressor of visible phenotype of chicoflp147E/chico1

NOT Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

foxo25 has eye phenotype, enhanceable by Akt152

foxo25 has ommatidium phenotype, enhanceable by Akt152

foxo25 has eye phenotype, enhanceable by Akt157

foxo25 has ommatidium phenotype, enhanceable by Akt157

foxo25 has eye phenotype, enhanceable by Akt104226

foxo25 has ommatidium phenotype, enhanceable by Akt104226

NOT Enhanced by
Statement
Reference
NOT suppressed by
Statement
Reference

foxo25 has eye phenotype, non-suppressible by Akt104226

foxo25 has ommatidium phenotype, non-suppressible by Akt104226

foxo25 has eye phenotype, non-suppressible by Akt152

foxo25 has ommatidium phenotype, non-suppressible by Akt152

foxo25 has eye phenotype, non-suppressible by Akt157

foxo25 has ommatidium phenotype, non-suppressible by Akt157

Enhancer of
Statement
Reference

foxo25 is an enhancer of eye phenotype of Akt152

foxo25 is an enhancer of ommatidium phenotype of Akt152

foxo25 is an enhancer of eye phenotype of Akt157

foxo25 is an enhancer of ommatidium phenotype of Akt157

foxo25 is an enhancer of eye phenotype of Akt104226/Akt104226

NOT Enhancer of
Statement
Reference

foxo25/foxo21 is a non-enhancer of adult thorax phenotype of Pink1B9

foxo25/foxo21 is a non-enhancer of mitochondrion | adult stage phenotype of Pink1B9

foxo25/foxo21 is a non-enhancer of indirect flight muscle phenotype of Pink1B9

foxo25/foxo21 is a non-enhancer of wing blade phenotype of Pink1B9

Suppressor of
Statement
Reference

foxo25/foxo21 is a suppressor of escort cell phenotype of InRE19/InR339

foxo25 is a suppressor of eye phenotype of Akt104226/Akt104226

foxo25 is a suppressor of eye phenotype of Akt152

foxo25 is a suppressor | partially of ommatidium phenotype of Akt152

foxo25 is a suppressor of eye phenotype of Akt157

foxo25 is a suppressor | partially of ommatidium phenotype of Akt157

foxo25/foxo[+] is a suppressor of male germline stem cell | germline clone phenotype of pieE1-16

foxo25/foxo[+] is a suppressor of eye disc | somatic clone phenotype of pieE1-16

foxo25/foxo[+] is a suppressor of eye phenotype of Hsap\APPAβ42.GMR.Tag:SS(rPENK)

foxo25/foxo21 is a suppressor of wing phenotype of chicoflp147E/chico1

NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The decreased number of escort cells observed in the germarium of InRE19/InR339 transheterozygotes is suppressed by additional foxo21/foxo25 transheterozygosity.

Presence of foxo25/foxo21 significantly suppresses increased survival in response to rotenone or paraquat in Trap1EY21851/Trap1EY21851 flies. Presence of foxo25/foxo21 suppresses rescue via feeding of G-TTP of locomotor defects in Pink1B9/Pink1B9 flies.

The reduction in ommatidium area seen in either mutant alone is enhanced in Akt104226 foxo25 double mutant flies. As in the foxo25 mutant alone, ommatidium number is increased.

The reduction in ommatidium area seen in either mutant alone is enhanced in Akt157 foxo25 double mutant flies. As in the foxo25 mutant alone, ommatidium number is increased.

The reduction in ommatidium area seen in either mutant alone is enhanced in Akt152 foxo25 double mutant flies. As in the foxo25 mutant alone, ommatidium number is increased.

One copy of foxo25 significantly suppresses the male germline stem cell (GSC) loss and reduced GSC division rate seen in pieE1-16 mutant clones.

One copy of foxo25 significantly suppresses the increased apoptosis seen in pieE1-16 mutant eye disc clones, and clones are seen in adult eyes.

Expression of Sirt1Scer\UAS.cGa driven by Scer\GAL4arm.PU partially suppresses phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in (3 day old) Pink1B9/Y flies. Presence of foxo25/foxo21 almost completely nullifies the suppressive effect of Scer\GAL4arm.PU>Sirt1Scer\UAS.cGa on phenotypes in Pink1B9/Y flies.

foxo25/foxo21 does not significantly enhance phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in Pink1B9/Y flies.

Pink1B9/Y does not enhance dopaminergic neuron loss (in the DL1 cluster) in foxo21/foxo25 flies.

Lipid levels are completely restored in Sik348 ; foxo21/foxo25 double mutant flies.

Neuroblasts are detected in the mushroom body of 2 week old and even in 1 month old adults simultaneously co-expressing rprmiRNA.RHG.Scer\UAS, WmiRNA.RHG.Scer\UAS and grimmiRNA.RHG.Scer\UAS (from the P{UAS-RHG.miRNA} transgene) under the control of Scer\GAL4wor.PA in a foxo25 background (mushroom body neuroblasts are not seen in wild-type adults and are not seen at these late time points in adults expressing the P{UAS-RHG.miRNA} transgene under the control of Scer\GAL4wor.PA in a wild-type background or in foxo25 single mutant adults). Some of the 2 week old mutant mushroom body neuroblasts are large in size and generate many new progeny. There is a strong correlation between mushroom body neuroblast cell size and progeny number in 2 week old mutant adults, but not in younger mutant adults. In 1 month old mutant adults, some of the progeny of these neuroblasts show normal axon projections through the mushroom body pedunculus and some mistarget, bifurcating prematurely and projecting anterior to the pedunculus.

A significant recovery of eye pigmentation and structure is seen in flies expressing Sir2Scer\UAS.cGa under the control of Scer\GAL4GMR.PU in a foxo25/foxo25 background.

A foxo25 heterozygous background has no effect on survival or lipid accumulation in starved TORC25-3 homozygotes.

Removal of both copies of foxo (foxo25/foxo21) is lethal in TORC25-3 homozygotes.

TORCScer\UAS.cWa over-expressing flies (driven by Scer\GAL4GMR.PF) in which foxo is reduced or eliminated though a foxo25/foxo21 background still exhibit a rough eye phenotype.

foxo25 Akt11 double homozygotes show considerable lethality; most animals die before the pupal stage, with most of the remaining animals dying as pupae and only a few escapers eclosing as adults. 71% of pupae still contain intact salivary glands at 20 hours after puparium formation (this is approximately 6 hours after the glands are normally destroyed in wild-type animals).

Ectopic expression of ThorScer\UAS.cTa, under the control of Scer\GAL4Act5C can completely suppress the sensitivity of foxo21/foxo25 flies to oxidative stress (median life-span of 56.8 hours, 39.7% survival rate after 60 hours exposure to 5% hydrogen peroxide).

The increase in average and maximum lifespan seen in pucE69/+ adults is dominantly suppressed by foxo25. The reduction in body weight seen in 1 day old hepAct.Scer\UAS; Scer\GAL4Ilp2.PR adults is partially supressed by foxo25/+.

Xenogenetic Interactions
Statement
Reference

The ability of 0.5mM Psammaplysene A to suppress the eye degeneration phenotype caused by expression of Hsap\ARQ52.Scer\UAS under the control of Scer\GAL4GMR.PU (in the presence of dihydrotestosterone) is suppressed if the flies are also heterozygous for foxo25.

Complementation and Rescue Data
Comments

Expression of foxoT:SV5\V5 rescues the circadian arrhythmia seen in foxo21/foxo25 mutants. Phototaxis is not affected by paraquat treatment in these mutants.

Expression of foxoScer\UAS.cFa under the control of Scer\GAL4Lk6-DJ634 rescues the circadian arrhythmia seen in foxo21/foxo25 mutants. Phototaxis is not affected by paraquat treatment in these mutants.

Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (12)
References (43)