aru8.128 flies display a robust increase in ethanol sensitivity. aru8.128 flies are healthy, fertile, and the gross morphology of their brains appear normal. aru8.128 flies exhibit a normal rate of ethanol absorption indicating that their increased sensitivity is not due to altered ethanol pharmacokinetics. However, this increase in ethanol sensitivity can be reversed by adult social isolation.
The time for 50% of aru8.128 mutant flies to reach sedation (ST50) is significantly decreased compared to wild-type controls (in a loss-of-righting reflex assay). aru8.128 mutant flies have a normal right reflex in the absence of ethanol and show normal baseline locomotor activity and startle-induced climbing.
aru8.128 heterozygous mutants show enhanced ethanol sensitivity.
aru8.128 flies exhibit normal circadian rhythms.
aru8.128 mutant larvae show a significant increase in the number of synaptic boutons (by 36.6% compared to controls), whose structure and morphology appear normal. The PDF neurons of adult aru8.128 flies also show a similar increase in synaptic terminals (by 29.8% compared to controls), showing that aru regulates synapse number in both larval and adult neurons. Social isolation of aru8.128 flies normalizes the number of synaptic terminals of PDF neurons.
After conditioning for twenty minutes with 41[o]C negative reinforcement, place memory levels are reduced ~30-35% in aru8.128 flies compared to the performance of canton-S wild-type flies. aru8.128 flies in a Berlin genetic background exhibit a non-significant reduction in memory performance.
aru8.128 flies exhibit a reduced olfactory memory after three minutes following training in a wild-type Berlin genetic background. In this background, aru8.128 flies exhibit a ~40% reduction in memory compared to Berlin flies memory performance. aru8.128 flies in a Canton-S background have memory level that are similar to the Canton-S performance levels. Thus, in olfactory classical conditioning, the aru8.128 allele can influence memory levels, but this depends on the genetic background in which it is tested.
aru8.128 flies exhibit wild-type abilities to sense and avoid odours, electric shock, and high temperatures.