FlyBase Allele Report: Dmel\aru[8.128]

General Information

Symbol

Dmel\aru^8.128

Species

D. melanogaster

Name

FlyBase ID

FBal0261089

Feature type

allele

Associated gene

Dmel\aru

Associated Insertion(s)

P{GawB}aru^8.128

Carried in Construct

Also Known As

aru^8-128

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Eddison et al., 2011)

Progenitor genotype

Associated Insertion(s)

P{GawB}aru^8.128

Cytology

Description

P-element mutagenesis results in a P-element being inserted in the 5' region of aru.

(Eddison et al., 2011)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Eddison et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates alcohol use disorder

modeled by hppy^17-51

(Eddison et al., 2011)

model of alcohol use disorder

is ameliorated by Akt^EY10012

(Eddison et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory

(Laferriere et al., 2011)

abnormal smell perception

(Laferriere et al., 2011)

chemical sensitive

(Eddison et al., 2011)

Phenotype Manifest In

bouton | increased number

(Eddison et al., 2011)

Detailed Description

Statement

Reference

aru^8.128 flies display a robust increase in ethanol sensitivity. aru^8.128 flies are healthy, fertile, and the gross morphology of their brains appear normal. aru^8.128 flies exhibit a normal rate of ethanol absorption indicating that their increased sensitivity is not due to altered ethanol pharmacokinetics. However, this increase in ethanol sensitivity can be reversed by adult social isolation.

The time for 50% of aru^8.128 mutant flies to reach sedation (ST50) is significantly decreased compared to wild-type controls (in a loss-of-righting reflex assay). aru^8.128 mutant flies have a normal right reflex in the absence of ethanol and show normal baseline locomotor activity and startle-induced climbing.

aru^8.128 heterozygous mutants show enhanced ethanol sensitivity.

aru^8.128 flies exhibit normal circadian rhythms.

aru^8.128 mutant larvae show a significant increase in the number of synaptic boutons (by 36.6% compared to controls), whose structure and morphology appear normal. The PDF neurons of adult aru^8.128 flies also show a similar increase in synaptic terminals (by 29.8% compared to controls), showing that aru regulates synapse number in both larval and adult neurons. Social isolation of aru^8.128 flies normalizes the number of synaptic terminals of PDF neurons.

(Eddison et al., 2011)

After conditioning for twenty minutes with 41[o]C negative reinforcement, place memory levels are reduced ~30-35% in aru^8.128 flies compared to the performance of canton-S wild-type flies. aru^8.128 flies in a Berlin genetic background exhibit a non-significant reduction in memory performance.

aru^8.128 flies exhibit a reduced olfactory memory after three minutes following training in a wild-type Berlin genetic background. In this background, aru^8.128 flies exhibit a ~40% reduction in memory compared to Berlin flies memory performance. aru^8.128 flies in a Canton-S background have memory level that are similar to the Canton-S performance levels. Thus, in olfactory classical conditioning, the aru^8.128 allele can influence memory levels, but this depends on the genetic background in which it is tested.

aru^8.128 flies exhibit wild-type abilities to sense and avoid odours, electric shock, and high temperatures.

(Laferriere et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

aru^8.128 has chemical sensitive phenotype, non-suppressible by Scer\GAL4^elav-C155/rl^Sem.C.UAS

(Eddison et al., 2011)

aru^8.128 has chemical sensitive phenotype, non-suppressible by hppy^17-51

(Eddison et al., 2011)

aru^8.128 has chemical sensitive phenotype, non-suppressible by Scer\GAL4^elav-C155/Pi3K92E^A2860C.UAS

(Eddison et al., 2011)

Suppressor of

Statement

Reference

aru^8.128 is a suppressor of chemical resistant phenotype of Scer\GAL4^elav-C155, rl^Sem.C.UAS

(Eddison et al., 2011)

aru^8.128 is a suppressor of chemical resistant phenotype of hppy^17-51

(Eddison et al., 2011)

aru^8.128 is a suppressor of chemical resistant phenotype of Pi3K92E^A2860C.UAS, Scer\GAL4^elav-C155

(Eddison et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Flies over-expressing rl^{Sem.C.Scer\UAS} in neurons with Scer\GAL4^elav-C155 in a aru^8.128 background shows increased sensitivity to ethanol sedation that is not significantly different from that of aru^8.128 flies, indicating that a aru^8.128 background suppresses the decreased ethanol sensitivity caused by overexpression of rl^{Sem.C.Scer\UAS}.

The presence of a aru^8.128 background suppresses the ethanol resistance found in hppy^17-51 mutants.

Flies over-expressing Pi3K92E^{A2860C.Scer\UAS} in neurons (with Scer\GAL4^elav-C155) in an aru^8.128 background show increased ethanol sensitivity, indicating suppression of the Pi3K92E^{A2860C.Scer\UAS} phenotype.

(Eddison et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

aru^8-128

(Jacomin et al., 2021, Brent and Rajan, 2020, Laferriere et al., 2011)

aru^8.128

(Jacomin et al., 2021, Eddison et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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