FlyBase Allele Report: Dmel\bam[UASp.GFP]

General Information

Symbol

Dmel\bam^UASp.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0144431

Feature type

allele

Associated gene

Dmel\bam

Associated Insertion(s)

Carried in Construct

P{UASp-bam.GFP}

Also Known As

UAS-bam-GFP

Key Links

Transgenic product class

wild_type

(Chen and McKearin, 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chen and McKearin, 2003)

Mutagen

in vitro construct

(Chen and McKearin, 2003)

Progenitor genotype

Carried in construct

P{UASp-bam.GFP}

Cytology

Description

UASp regulatory sequences drive expression of bam tagged with GFP.

(Chen and McKearin, 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

bam

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | spermatogenesis, with Scer\GAL4^{VP16.nanos.UTR}

(Yuen et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of bam^UASp.GFP under the control of Scer\GAL4^VP16.nos.UTR results in variably penetrant germ cell loss from the niche; in testes completely lacking a germline, ectopic somatic cyst stem cells can be observed many cell diameters away from the hub, and few differentiated cyst cells.

(Yuen et al., 2021)

Scer\GAL4^nos.PU-mediated expression of bam^{Scer\UAS.P\T.T:Avic\GFP} promotes germline stem cell (GSC) differentiation, thereby eliminating all GSCs.

(Chen et al., 2014)

bam^{Scer\UAS.P\T.T:Avic\GFP}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} adult males lack germline stem cells.

(Kawase et al., 2004)

Expression of bam^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in tiny testes with no detectable germ cells. The somatic hub is greatly enlarged in the testes of these males.

(Schulz et al., 2004)

Adult ovaries of females expressing bam^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} contain only a few maturing eggs but no other germline cells. The testes of males expressing bam^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} are shriveled. Oogenesis is normal in females expressing bam^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{bam.T:Hsim\VP16}.

(Chen and McKearin, 2003)

Expression of bam^{Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in testes which are dramatically reduced in size, lack early germ cells and contain only mature spermatids.

(Shivdasani and Ingham, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/bam^UASp.GFP is a suppressor of decreased cell number | somatic clone | spermatogenesis phenotype of Tsc1²⁹

(Yuen et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^nanos.PU, bam^UASp.GFP has male germline stem cell phenotype, suppressible by tut¹/tut³

(Chen et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/bam^UASp.GFP is a suppressor of cyst progenitor cell | somatic clone phenotype of Tsc1²⁹

(Yuen et al., 2021)

Scer\GAL4^{VP16.nanos.UTR}/bam^UASp.GFP is a suppressor of cyst cell of testis | somatic clone phenotype of Tsc1²⁹

(Yuen et al., 2021)

bam^UASp.GFP/Scer\GAL4^VP16.bam is a suppressor of spermatogonium | increased number phenotype of Doa^HD/Doa^Dem

(Zhao et al., 2013)

bam^UASp.GFP/Scer\GAL4^vas.2.6 is a suppressor of spermatogonium | increased number phenotype of Doa^HD/Doa^Dem

(Zhao et al., 2013)

Other

Statement

Reference

Ras85D^V12.cZa.UASp, Scer\GAL4^{VP16.nanos.UTR}, bam^UASp.GFP has egg chamber | increased number phenotype

(Zhang et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^nos.PU-mediated expression of bam^{Scer\UAS.P\T.T:Avic\GFP} in a tut¹/tut³ background results in a tut mutant phenotype.

(Chen et al., 2014)

Expression of bam^{Scer\UAS.P\T.T:Avic\GFP} under the control of either Scer\GAL4^{bam.T:Hsim\VP16} or Scer\GAL4^vas.2.6 promotes the differentiation of spermatogonia to spermatocytes in Doa^Dem/Doa^HD testes.

(Zhao et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

bam^UASp.GFP/Scer\GAL4^VP16.bam rescues bam^Δ86

(Chen and McKearin, 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

bam^{Scer\UAS.P\T.T:Avic\GFP}

bam^UASp.GFP

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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