FlyBase Allele Report: Dmel\Doa[HD]

General Information

Symbol

Dmel\Doa^HD

Species

D. melanogaster

Name

FlyBase ID

FBal0002719

Feature type

allele

Associated gene

Dmel\Doa

Associated Insertion(s)

copia{}Doa^HD

Carried in Construct

Also Known As

Doa^HD1, Doa^HD2

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

PM hybrid dysgenesis

transposable element activity

(Yun et al., 1994, Rabinow and Birchler, 1989)

Progenitor genotype

Associated Insertion(s)

copia{}Doa^HD

Cytology

Polytene chromosomes normal.

(Rabinow and Birchler, 1989)

Description

The copia{} element is inserted 177 nucleotides 3' to the first non-coding exon of Doa and 231 nucleotides 5' to the 55kD transcript's initiating AUG.

(Kpebe and Rabinow, 2008)

An uncharacterised 5kb transposable element in inserted within an intron.

(Yun et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:28,890,926..28,890,926 [-]

(Kpebe and Rabinow, 2008, Yun et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sex-determination (with Doa⁵)

(Kpebe and Rabinow, 2008)

abnormal sex-determination (with Doa^Dem)

(Fan et al., 2014)

chemical resistant | dominant

(James et al., 2009)

lethal (with Df(3R)3450)

(Yun et al., 2000)

lethal (with Doa^01705b)

(Yun et al., 2000)

lethal | recessive

(Rabinow et al., 1993, Rabinow and Birchler, 1989)

male sterile (with Doa^Dem)

(Zhao et al., 2013)

viable (with Doa^Dem)

(Zhao et al., 2013)

visible | dominant

(Rabinow and Birchler, 1990)

Phenotype Manifest In

spermatogonium | germline clone | increased number

(Zhao et al., 2013)

spermatogonium | increased number (with Doa^Dem)

(Zhao et al., 2013)

Detailed Description

Statement

Reference

Doa^HD/Doa^Dem mutant flies show a mild female to male somatic sex transformation phenotype. Very slight asymmetry is seen and there are a few missing vaginal bristles.

(Fan et al., 2014)

Doa^Dem/Doa^HD testes are filled with early stage germ cells.

Homozygous germline clones result in early germ cell over-proliferation in testes. The overproliferating cells have branched fusomes, indicating that they are spermatogonia.

(Zhao et al., 2013)

Doa^HD heterozygous adults show significantly reduced sensitity to paraquat.

(James et al., 2009)

In Doa^HD/Doa^s2784 mutants where the Doa^HD allele is from the mother blackened areas are seen on the wings. When the Doa^s2784 allele comes from the mother the mutants die at or prior to the pupal stage.

In Doa^HD/Doa⁵ mutants where the Doa^HD allele is from the mother there is a strong transformation of females towards males, with male colouration of tergites 5 and 6, male sex combs on the prothoracic leg, and a normal but rotated female genital plate. When the Doa⁵ allele comes from the mother there are no survivors.

(Kpebe and Rabinow, 2008)

Heterozygotes with Doa⁷ have ommatidia of varying size and disorganization of the interommatidial bristles. Random interruption of the normal lattice of pigment cells and random vacuolization of the retina and pigment cells are restored to at least normal levels (suppression of w^a).

(Yun et al., 1994)

Crosses between Doa^HD and Doa⁹ heterozygotes indicate that Doa is not an embryonic lethal, and is probably lethal during early larval stages. Rare Doa^HD/Doa⁶ or Doa^HD/Doa⁷ escapers of lethality are found at a frequency of approximately 0.5%. Doa^HD/Doa⁶ escapers are more frequently female than male (ratio 4:1 ) and the male escapers are sterile despite having motile sperm.

(Rabinow and Birchler, 1989)

Heterozygotes with Doa⁶ or Doa⁷ survive rarely and when they do, w^a is darkened to nearly wild-type color; w^sp55 reacts in the opposite manner, becoming nearly white; eyes of escapers have disarranged facets. Males of these heteroallelic combinations involving Doa⁶ are sterile despite having motile sperm; females and both sexes involving Doa⁷ are weakly fertile.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Doa^HD/Doa^Dem has abnormal sex-determination phenotype, enhanceable by Srrm1^B103

(Fan et al., 2014)

Doa^HD/Doa^Dem has abnormal sex-determination phenotype, enhanceable by Srrm1^GE25979

(Fan et al., 2014)

Suppressed by

Statement

Reference

Doa^HD/Doa^Dem has male sterile phenotype, suppressible | partially by Hsap\CLK2^UAS.cZa/Scer\GAL4^VP16.bam

(Zhao et al., 2013)

Doa^HD/Doa^Dem has male sterile phenotype, suppressible | partially by Scer\GAL4^VP16.bam/Mmus\Clk2^UAS.cZa

(Zhao et al., 2013)

Suppressor of

Statement

Reference

Doa[+]/Doa^HD is a suppressor | partially of lethal phenotype of Scer\GAL4^da.PU, Srrm1^GE25979

(Fan et al., 2014)

Doa^HD/Doa^Dem is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

NOT Suppressor of

Statement

Reference

Doa^HD/Doa^Dem is a non-suppressor of lethal phenotype of Scer\GAL4^da.PU, Srrm1^GE25979

(Fan et al., 2014)

Other

Statement

Reference

Doa^HD/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has partially lethal - majority die phenotype

(Fan et al., 2014)

Doa^HD/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has short lived phenotype

(Fan et al., 2014)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has visible | dominant phenotype

(Fan et al., 2010)

Doa^HD, E(gl)3C^unspecified has viable phenotype

(Yun et al., 2000)

Doa^HD, E(gl)3C^unspecified has fertile phenotype

(Yun et al., 2000)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Doa⁷/Doa^HD has phenotype, non-enhanceable by Ras85D^e2F

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-enhanceable by gl²

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-enhanceable by gl^60j

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-enhanceable by rl¹

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-enhanceable by rl^Sem

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-enhanceable by sina¹

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by Egfr^E1

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by Ras85D^e2F

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by gl²

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by gl^60j

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by rl¹

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by rl^Sem

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by sina¹

(Yun et al., 2000)

Suppressed by

Statement

Reference

Doa^HD/Doa^Dem has spermatogonium | increased number phenotype, suppressible by bam^UASp.GFP/Scer\GAL4^VP16.bam

(Zhao et al., 2013)

Doa^HD/Doa^Dem has spermatogonium | increased number phenotype, suppressible by bam^UASp.GFP/Scer\GAL4^vas.2.6

(Zhao et al., 2013)

Doa^HD/Doa^Dem has spermatogonium | increased number phenotype, suppressible by Hsap\CLK2^C274Y.UAS/Scer\GAL4^VP16.bam

(Zhao et al., 2013)

NOT suppressed by

Statement

Reference

Doa^HD/Doa^Dem has spermatogonium | increased number phenotype, non-suppressible by Scer\GAL4^VP16.bam/Hsap\CLK2^K192A.UAS

(Zhao et al., 2013)

Doa⁷/Doa^HD has phenotype, non-suppressible by Ras85D^e2F

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-suppressible by gl²

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-suppressible by gl^60j

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-suppressible by rl¹

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-suppressible by rl^Sem

(Yun et al., 2000)

Doa⁷/Doa^HD has phenotype, non-suppressible by sina¹

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by Egfr^E1

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by Ras85D^e2F

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by gl²

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by gl^60j

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by rl¹

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by rl^Sem

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by sina¹

(Yun et al., 2000)

Enhancer of

Statement

Reference

Doa[+]/Doa^HD is an enhancer of male genitalia phenotype of Scer\GAL4^esg.PU, Srrm1^GE25979

(Fan et al., 2014)

Doa^HD/Doa^Dem is an enhancer of male genitalia phenotype of Scer\GAL4^esg.PU, Srrm1^GE25979

(Fan et al., 2014)

Doa^HD is an enhancer of phenotype of w^sp55

(Rabinow and Birchler, 1989)

NOT Enhancer of

Statement

Reference

Doa^HD is a non-enhancer of phenotype of w^a57i11

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^a58l

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^a2

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^r

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^IR5

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of y²

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^a3

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^sp81d

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^a59k1

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^sp2

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^E

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^IR3

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^ec3

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^apl

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^IR2

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^sat

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^bl

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^Bwx

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^a4

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^col

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^e2

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^IR1

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^co

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of wⁱ

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^IR4

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^IR6

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^mo

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^h

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^bf2

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^zm

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^bf

(Rabinow and Birchler, 1989)

Doa^HD is a non-enhancer of phenotype of w^e

(Rabinow and Birchler, 1989)

Suppressor of

Statement

Reference

Doa^HD/Doa^Dem is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Doa^HD is a suppressor of phenotype of w^aM

(Rabinow and Birchler, 1989)

Doa^HD is a suppressor of phenotype of w^aR84h

(Rabinow and Birchler, 1989)

Doa^HD is a suppressor of phenotype of w^a

(Rabinow and Birchler, 1989)

NOT Suppressor of

Statement

Reference

Doa^HD is a non-suppressor of phenotype of w^a57i11

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^a58l

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^sp2

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^E

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^ec3

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^IR2

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^bl

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^col

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^e2

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^IR4

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^IR3

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^IR6

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^mo

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^Bwx

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^a3

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^a4

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^apl

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^bf

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^bf2

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^IR1

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^co

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^e

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^h

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of wⁱ

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^sat

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^zm

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of y²

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^a2

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^r

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^IR5

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^sp81d

(Rabinow and Birchler, 1989)

Doa^HD is a non-suppressor of phenotype of w^a59k1

(Rabinow and Birchler, 1989)

Other

Statement

Reference

Doa^HD/Doa^Dem, eEF1γ^GE29510 has posterior crossvein | male phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has wing phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has eye phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has wing vein | ectopic phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has female genitalia phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has crossvein phenotype

(Fan et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

SRm160^B103 strongly enhances the female to male somatic sex transformation phenotype seen in Doa^HD/Doa^Dem mutant flies. The male genital arch and "claspers" are seen in XX individuals.

SRm160^GE25979 strongly enhances the female to male somatic sex transformation phenotype seen in Doa^HD/Doa^Dem mutant flies. The male genital arch and "claspers" are seen in XX individuals.

Doa^HD/Doa^Dem does not suppress the lethality seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^da.PU.

Doa^HD/Doa^Dem almost completely suppresses the smooth, glassy eye seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU. The ommatidial disruption is partially suppressed: the various cell types and layers are all identifiable albeit somewhat perturbed. Although differentiation pigment cells are observed and cone cells secrete a cornea of normal appearance, the pigment and photoreceptor cells fail to elongate to form a normal retina. There is a reduction in the levels of apoptosis (caspase activation).

One copy of Doa^HD partially suppresses the lethality seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^da.PU. The viable escapers do not display any cuticular phenotypes.

One copy of Doa^HD enhances the loss of external genitalia phenotype seen in males expressing SRm160^GE25979 under the control of Scer\GAL4^esg.PU.

Very few animals survive when SRm160^GE25979 is expressed under the control of Scer\GAL4^esg.PU in a Doa^HD/Doa^Dem mutant background, but those that do survive completely lack genitalia and analia and die shortly following eclosion.

(Fan et al., 2014)

Expression of bam^{Scer\UAS.P\T.T:Avic\GFP} under the control of either Scer\GAL4^{bam.T:Hsim\VP16} or Scer\GAL4^vas.2.6 promotes the differentiation of spermatogonia to spermatocytes in Doa^Dem/Doa^HD testes.

(Zhao et al., 2013)

Doa^HD Ef1γ^GE29510/Doa^Dem flies show a number of defects, including ectopic wing venation, failure of either of the crossveins to attach (particularly the posterior crossvein in males), aberrantly formed crossveins, occasional eye roughness, curved wings and rarely, partial rotation of the female genitalia.

(Fan et al., 2010)

Doa^HD in combination with E(gl)3C^unspecified results in flies with normal eyes, wings and bristles. No suppression of w^a is seen in these flies.

(Yun et al., 2000)

Rare Doa^HD/Doa⁶ or Doa^HD/Doa⁷ escapers show extreme suppression of the w^a phenotype, such that eyes, Malpighian tubules, ocelli and testes are essentially wild-type in colour. They have disordered eye facets and scutellar bristles are often missing or abnormal. Male escapers carrying w^sp55 have bleach-white eyes.

(Rabinow and Birchler, 1989)

Xenogenetic Interactions

Statement

Reference

Expression of either Hsap\CLK2^Scer\UAS.cZa or Mmus\Clk2^Scer\UAS.cZa under the control of Scer\GAL4^{bam.T:Hsim\VP16} partially rescues the sterility of Doa^Dem/Doa^HD males.

Expression of Hsap\CLK2^{K192A.Scer\UAS} under the control of Scer\GAL4^{bam.T:Hsim\VP16} is unable to promote the differentiation of spermatogonia in Doa^Dem/Doa^HD testes.

Expression of Hsap\CLK2^{C274Y.Scer\UAS} under the control of Scer\GAL4^{bam.T:Hsim\VP16} is able to promote the differentiation of spermatogonia in Doa^Dem/Doa^HD testes.

(Zhao et al., 2013)

Complementation and Rescue Data

Rescued by

Doa^HD/Doa^Dem is rescued by Scer\GAL4^VP16.bam/Doa^{69.long3'UTR.UAS}

(Zhao et al., 2013)

Doa^HD/Doa^Dem is rescued by Scer\GAL4^VP16.bam/Doa^UAS.PS

(Zhao et al., 2013)

Partially rescued by

Doa^HD/Doa^Dem is partially rescued by Doa^UAS.PK/Scer\GAL4^VP16.bam

(Zhao et al., 2013)

Doa^HD/Doa^Dem is partially rescued by Doa^{139-511.UAS.PS.Tag:FLAG}/Scer\GAL4^VP16.bam

(Zhao et al., 2013)