FlyBase Allele Report: Dmel\Srrm1[GE25979]

General Information

Symbol

Dmel\Srrm1^GE25979

Species

D. melanogaster

Name

FlyBase ID

FBal0301137

Feature type

allele

Associated gene

Dmel\Srrm1

Associated Insertion(s)

P{EP}Srrm1^GE25979

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Fan et al., 2014)

Progenitor genotype

Associated Insertion(s)

P{EP}Srrm1^GE25979

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:13,037,011..13,037,011

(Fan et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile, with Scer\GAL4^esg.PU

(Fan et al., 2014)

fertile (with Srrm1^G18603)

(Fan et al., 2014)

lethal, with Scer\GAL4^da.PU

(Fan et al., 2014)

lethal - all die before end of pupal stage | male limited | heat sensitive, with Scer\GAL4^GMR.PU

(Fan et al., 2014)

male fertile, with Scer\GAL4^esg.PU

(Fan et al., 2014)

partially lethal - majority die | female limited | heat sensitive, with Scer\GAL4^GMR.PU

(Fan et al., 2014)

some die during embryonic stage, with Scer\GAL4^da.PU

(Fan et al., 2014)

some die during larval stage, with Scer\GAL4^da.PU

(Fan et al., 2014)

some die during pupal stage, with Scer\GAL4^da.PU

(Fan et al., 2014)

some die during pupal stage | male limited | heat sensitive, with Scer\GAL4^GMR.PU

(Fan et al., 2014)

visible, with Scer\GAL4^GMR.PU

(Fan et al., 2014)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PU

(Fan et al., 2014)

eye | female limited | heat sensitive, with Scer\GAL4^GMR.PU

(Fan et al., 2014)

male analia, with Scer\GAL4^esg.PU

(Fan et al., 2014)

male genitalia, with Scer\GAL4^esg.PU

(Fan et al., 2014)

ommatidium, with Scer\GAL4^GMR.PU

(Fan et al., 2014)

testis, with Scer\GAL4^esg.PU

(Fan et al., 2014)

Detailed Description

Statement

Reference

Expression of SRm160^GE25979 under the control of Scer\GAL4^da.PU results in lethality at 25[o]C. A few female escapers are seen, but no males. These females show no visible phenotypes.

Expression of SRm160^GE25979 in the genital disc under the control of Scer\GAL4^esg.PU yields mostly normal progeny at 25[o]C and both sexes are fertile. 10% of the males show abnormal genital structure, including rotated genitals or extra appendages or completely lacking external genitalia and analia. Sex combs and pigmentation are both normal. Animals with aberrant genital morphology have testes that are poorly developed and have failed to elongate, but these contain sperm of normal morphology.

Expression of SRm160^GE25979 under the control of Scer\GAL4^GMR.PU results in rough and shiny eyes, observable at 25[o]C with males more strongly affected. Loss of differentiation and complete disorganisation of the ommatidial array is seen compared to wild type controls. No sign of the pigment cell lattice is visible but the lens is present. Formation of the lamina is also abnormal in Scer\GAL4^GMR.PU>SRm160^GE25979 flies compared with wild type. Increased levels of apoptosis (activated caspases) are seen at 25[o]C compared to controls. A more severe phenotype is seen at 29[o]C: very few flies survive and those that do are females with glassy eyes with little pigment. Male lethality occurs during pupation.

SRm160^G18603/SRm160^GE25979 mutant flies are normal in all cuticular phenotypes and fertile.

(Fan et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Srrm1^GE25979 has visible phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PU

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has visible phenotype, suppressible by Dronc^I24/Dronc[+]

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has visible phenotype, suppressible by Dronc[+]/Dronc^I29

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has visible phenotype, suppressible by Dcp-1^k05606/Dcp-1[+]

(Fan et al., 2014)

Scer\GAL4^da.PU, Srrm1^GE25979 has lethal phenotype, suppressible | partially by Doa[+]/Doa^HD

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has visible phenotype, suppressible | partially by Doa[+]/Doa^Dem

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has visible phenotype, suppressible | partially by Doa^HD/Doa^Dem

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has visible phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has visible phenotype, suppressible | partially by Df(3L)H99/+

(Fan et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^da.PU, Srrm1^GE25979 has lethal phenotype, non-suppressible by Doa^HD/Doa^Dem

(Fan et al., 2014)

Enhancer of

Statement

Reference

Srrm1^GE25979 is an enhancer of abnormal sex-determination phenotype of Doa^HD/Doa^Dem

(Fan et al., 2014)

Other

Statement

Reference

Doa^HD/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has partially lethal - majority die phenotype

(Fan et al., 2014)

Doa^HD/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has short lived phenotype

(Fan et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^esg.PU, Srrm1^GE25979 has male genitalia phenotype, enhanceable by Doa[+]/Doa^HD

(Fan et al., 2014)

Scer\GAL4^esg.PU, Srrm1^GE25979 has male genitalia phenotype, enhanceable by Doa[+]/Doa^Dem

(Fan et al., 2014)

Scer\GAL4^esg.PU, Srrm1^GE25979 has male genitalia phenotype, enhanceable by Doa^HD/Doa^Dem

(Fan et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Srrm1^GE25979 has eye phenotype, suppressible by Df(3L)H99/+

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has eye phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PU

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has eye phenotype, suppressible by Dronc^I24/Dronc[+]

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has eye phenotype, suppressible by Dronc[+]/Dronc^I29

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has eye phenotype, suppressible by Dcp-1^k05606/Dcp-1[+]

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has eye phenotype, suppressible | partially by Doa[+]/Doa^Dem

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has eye phenotype, suppressible | partially by Doa^HD/Doa^Dem

(Fan et al., 2014)

Scer\GAL4^GMR.PU, Srrm1^GE25979 has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Fan et al., 2014)

Other

Statement

Reference

Doa[+]/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has female genitalia phenotype

(Fan et al., 2014)

Doa[+]/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has female analia phenotype

(Fan et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

SRm160^GE25979 strongly enhances the female to male somatic sex transformation phenotype seen in Doa^HD/Doa^Dem mutant flies. The male genital arch and "claspers" are seen in XX individuals.

One copy of Df(3L)H99 partially suppresses the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Diap1^Scer\UAS.cUa suppresses the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

One copy of Dronc^I24 slightly suppresses the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

One copy of Dronc^I29 slightly suppresses the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

One copy of Dcp-1^k05606 slightly suppresses the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

One copy of p53^5A-1-4 does not suppress the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

One copy of p53^11-1B-1 does not suppress the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

One copy of Doa^HD partially suppresses the lethality seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^da.PU. The viable escapers do not display any cuticular phenotypes.

Doa^HD/Doa^Dem does not suppress the lethality seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^da.PU.

One copy of Doa^Dem partially suppresses the ommatidial disruption seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU. The various cell types and layers are all identifiable albeit somewhat perturbed. Although differentiation pigment cells are observed and cone cells secrete a cornea of normal appearance, the pigment and photoreceptor cells fail to elongate to form a normal retina. There is a reduction in the levels of apoptosis (caspase activation).

Doa^HD/Doa^Dem almost completely suppresses the smooth, glassy eye seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU. The ommatidial disruption is partially suppressed: the various cell types and layers are all identifiable albeit somewhat perturbed. Although differentiation pigment cells are observed and cone cells secrete a cornea of normal appearance, the pigment and photoreceptor cells fail to elongate to form a normal retina. There is a reduction in the levels of apoptosis (caspase activation).

One copy of Doa^HD enhances the loss of external genitalia phenotype seen in males expressing SRm160^GE25979 under the control of Scer\GAL4^esg.PU.

One copy of Doa^Dem enhances the loss of external genitalia phenotype seen in males expressing SRm160^GE25979 under the control of Scer\GAL4^esg.PU. Unlike in Scer\GAL4^esg.PU>SRm160^GE25979 mutant flies alone, a small proportion of females lack external genitals and anal plates.

Very few animals survive when SRm160^GE25979 is expressed under the control of Scer\GAL4^esg.PU in a Doa^HD/Doa^Dem mutant background, but those that do survive completely lack genitalia and analia and die shortly following eclosion.

(Fan et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa strongly suppresses the eye roughness seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU.

(Fan et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

SRm160^GE25979

Srrm1^GE25979

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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