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General Information
Symbol
Dmel\DoaDem
Species
D. melanogaster
Name
Deus ex machina
FlyBase ID
FBal0033616
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    sequence variant
    Linked to:
    EcoRI-SalI restriction fragment
    Comment:

    Insertion site mapped to restriction fragment in Figure 4 of FBrf0074875, position of restriction fragment on reference sequence inferred by FlyBase curator

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Insertion breakpoint within an intron.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    DoaHD/DoaDem mutant flies show a mild female to male somatic sex transformation phenotype. Very slight asymmetry is seen and there are a few missing vaginal bristles.

    DoaDem/DoaHD testes are filled with early stage germ cells.

    Homozygous germline clones result in early germ cell over-proliferation in testes. The overproliferating cells have branched fusomes, indicating that they are spermatogonia.

    In DoaDem/Doa01705b mutant flies, the leg segments distal to the first tarsus often fail to elongate. The femur and claws form normally. Defects are seen in both the mesothoracic and metathoracic tarsi.

    The L5 wing vein in DoaRemγA/DoaDem mutant flies fails to attach to the anterior wing margin. Spurs are also seen in the posterior crossvein.

    The ovaries of DoaE786/DoaDem mutant flies appear normal, despite asymmetry in the female genital sex combs.

    In DoaDem/Doa198-14 mutants where the DoaDem allele is from the mother there are no survivors. When the Doa198-14 allele comes from the mother the flies are viable, but the postalar bristles are duplicated and the scutellar bristles are forked-like.

    In DoaDem/Doa04743 mutants where the DoaDem allele is from the mother only rare pupae eclose and weak adults die quickly.The flies exhibit rough eyes, missing bristles and an incomplete L5 wing vein. When the Doa04743 allele comes from the mother there are no survivors.

    Transheterozygotes with Doa01705b have rough eyes and duplicated scutellar bristles.

    XX ; Doaunspecified/DoaDem adults have subtle intersexual characteristics in sex combs, pigmentation and genitalia. Female-specific vaginal bristles are missing or irregular in most cases. XX ; Doa7/DoaDem adults have subtle intersexual characteristics.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference
    Suppressed by
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Statement
    Reference

    DoaHD/DoaDem is a non-suppressor of lethal phenotype of Srrm1GE25979, Scer\GAL4da.PU

    Other
    Phenotype Manifest In
    NOT Enhanced by
    Statement
    Reference

    DoaHD/DoaDem has phenotype, non-enhanceable by EgfrE1

    DoaHD/DoaDem has phenotype, non-enhanceable by Ras85De2F

    DoaHD/DoaDem has phenotype, non-enhanceable by gl2

    DoaHD/DoaDem has phenotype, non-enhanceable by gl60j

    DoaHD/DoaDem has phenotype, non-enhanceable by rl1

    DoaHD/DoaDem has phenotype, non-enhanceable by rlSem

    DoaHD/DoaDem has phenotype, non-enhanceable by sina1

    Suppressed by
    NOT suppressed by
    Statement
    Reference

    DoaHD/DoaDem has phenotype, non-suppressible by EgfrE1

    DoaHD/DoaDem has phenotype, non-suppressible by Ras85De2F

    DoaHD/DoaDem has phenotype, non-suppressible by gl2

    DoaHD/DoaDem has phenotype, non-suppressible by gl60j

    DoaHD/DoaDem has phenotype, non-suppressible by rl1

    DoaHD/DoaDem has phenotype, non-suppressible by rlSem

    DoaHD/DoaDem has phenotype, non-suppressible by sina1

    Enhancer of
    Statement
    Reference
    Suppressor of
    Statement
    Reference

    Doa[+]/DoaDem is a suppressor | partially of eye phenotype of Srrm1GE25979, Scer\GAL4GMR.PU

    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    SRm160B103 strongly enhances the female to male somatic sex transformation phenotype seen in DoaHD/DoaDem mutant flies. The male genital arch and "claspers" are seen in XX individuals.

    SRm160B103 produces a female to male somatic sex transformation phenotype in DoaHD/Doa7 mutant flies. The male genital arch and "claspers" are seen in XX individuals.

    SRm160GE25979 strongly enhances the female to male somatic sex transformation phenotype seen in DoaHD/DoaDem mutant flies. The male genital arch and "claspers" are seen in XX individuals.

    DoaHD/DoaDem does not suppress the lethality seen when SRm160GE25979 is expressed under the control of Scer\GAL4da.PU.

    DoaHD/DoaDem almost completely suppresses the smooth, glassy eye seen when SRm160GE25979 is expressed under the control of Scer\GAL4GMR.PU. The ommatidial disruption is partially suppressed: the various cell types and layers are all identifiable albeit somewhat perturbed. Although differentiation pigment cells are observed and cone cells secrete a cornea of normal appearance, the pigment and photoreceptor cells fail to elongate to form a normal retina. There is a reduction in the levels of apoptosis (caspase activation).

    One copy of DoaDem partially suppresses the ommatidial disruption seen when SRm160GE25979 is expressed under the control of Scer\GAL4GMR.PU. The various cell types and layers are all identifiable albeit somewhat perturbed. Although differentiation pigment cells are observed and cone cells secrete a cornea of normal appearance, the pigment and photoreceptor cells fail to elongate to form a normal retina. There is a reduction in the levels of apoptosis (caspase activation).

    One copy of DoaDem enhances the loss of external genitalia phenotype seen in males expressing SRm160GE25979 under the control of Scer\GAL4esg.PU. Unlike in Scer\GAL4esg.PU>SRm160GE25979 mutant flies alone, a small proportion of females lack external genitals and anal plates.

    Very few animals survive when SRm160GE25979 is expressed under the control of Scer\GAL4esg.PU in a DoaHD/DoaDem mutant background, but those that do survive completely lack genitalia and analia and die shortly following eclosion.

    Expression of bamScer\UAS.P\T.T:Avic\GFP under the control of either Scer\GAL4bam.T:Hsim\VP16 or Scer\GAL4vas.2.6 promotes the differentiation of spermatogonia to spermatocytes in DoaDem/DoaHD testes.

    DoaHD Ef1γGE29510/DoaDem flies show a number of defects, including ectopic wing venation, failure of either of the crossveins to attach (particularly the posterior crossvein in males), aberrantly formed crossveins, occasional eye roughness, curved wings and rarely, partial rotation of the female genitalia.

    DoaDem in combination with E(gl)3Cunspecified results in flies with normal eyes, wings and bristles. No suppression of wa is seen in these flies.

    Heterozygous traunspecified/+ adults have normal sexual differentiation. XX ; traunspecified/+ adults that are also carrying Doaunspecified/DoaDem are clearly intersexual with respect to the number or morphology of sex combs. XX; Doaunspecified traunspecified/DoaDem + adults have genitalia which have characteristics of both sexes, including male-like clasper bristles and anal pads. Pigmentation of abdominal tergites is intermediate to that of the normal male and female phenotypes. XY flies carrying traHsp83.PS develop phenotypically as nearly perfect females. This female differentiation is substantially reversed if the flies are also carrying Doaunspecified/DoaDem.

    Xenogenetic Interactions
    Statement
    Reference

    Expression of either Hsap\CLK2Scer\UAS.cZa or Mmus\Clk2Scer\UAS.cZa under the control of Scer\GAL4bam.T:Hsim\VP16 partially rescues the sterility of DoaDem/DoaHD males.

    Expression of Hsap\CLK2K192A.Scer\UAS under the control of Scer\GAL4bam.T:Hsim\VP16 is unable to promote the differentiation of spermatogonia in DoaDem/DoaHD testes.

    Expression of Hsap\CLK2C274Y.Scer\UAS under the control of Scer\GAL4bam.T:Hsim\VP16 is able to promote the differentiation of spermatogonia in DoaDem/DoaHD testes.

    Complementation and Rescue Data
    Comments

    Expression of either Doa69.long3'UTR.Scer\UAS or DoaScer\UAS.PS under the control of Scer\GAL4bam.T:Hsim\VP16 almost completely rescues the sterility of DoaDem/DoaHD males.

    Expression of either Doa139-511.Scer\UAS.PS.T:Zzzz\FLAG or DoaScer\UAS.PK under the control of Scer\GAL4bam.T:Hsim\VP16 partially rescues the sterility of DoaDem/DoaHD males.

    Expression of Doa1-138.Scer\UAS.PS.T:Zzzz\FLAG under the control of Scer\GAL4bam.T:Hsim\VP16 fails to rescue the sterility of DoaDem/DoaHD males.

    Expression of either DoaC275Y.Scer\UAS.PS.T:Zzzz\FLAG or DoaK193A.Scer\UAS.PS.T:Zzzz\FLAG under the control of Scer\GAL4bam.T:Hsim\VP16 is unable to promote the differentiation of spermatogonia in DoaDem/DoaHD testes.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (5)
    Reported As
    Name Synonyms
    Deus ex machina
    Secondary FlyBase IDs
      References (9)