FlyBase Allele Report: Dmel\Doa[Dem]

General Information

Symbol

Dmel\Doa^Dem

Species

D. melanogaster

Name

Deus ex machina

FlyBase ID

FBal0033616

Feature type

allele

Associated gene

Dmel\Doa

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Insertion breakpoint within an intron.

(Yun et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

sequence_variant

3R:28,899,086..28,900,903 [+]

Linked to:

EcoRI-SalI restriction fragment

Comment:

Insertion site mapped to restriction fragment in Figure 4 of FBrf0074875, position of restriction fragment on reference sequence inferred by FlyBase curator

(Yun et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sex-determination (with Doa^HD)

(Fan et al., 2014)

abnormal sex-determination | female | recessive

(Du et al., 1998)

lethal (with Df(3R)3450)

(Yun et al., 2000)

lethal | recessive

(Rabinow et al., 1993)

male sterile (with Doa^HD)

(Zhao et al., 2013)

viable (with Doa^HD)

(Zhao et al., 2013)

visible (with Doa^01705b)

(Yun et al., 2000)

visible | adult stage (with Doa^01705b)

(Kpebe and Rabinow, 2008)

visible | adult stage (with Doa^RemγA)

(Kpebe and Rabinow, 2008)

Phenotype Manifest In

female genitalia

(Du et al., 1998)

female terminalia sensillum

(Du et al., 1998)

mesothoracic leg (with Doa^01705b)

(Kpebe and Rabinow, 2008)

metathoracic leg (with Doa^01705b)

(Kpebe and Rabinow, 2008)

ommatidium (with Doa^01705b)

(Yun et al., 2000)

posterior crossvein (with Doa^RemγA)

(Kpebe and Rabinow, 2008)

scutellar bristle | increased number (with Doa^01705b)

(Yun et al., 2000)

sex comb | female limited (with Doa^E786)

(Kpebe and Rabinow, 2008)

spermatogonium | germline clone | increased number

(Zhao et al., 2013)

spermatogonium | increased number (with Doa^HD)

(Zhao et al., 2013)

wing (with Doa^RemγA)

(Kpebe and Rabinow, 2008)

wing vein L5 (with Doa^RemγA)

(Kpebe and Rabinow, 2008)

Detailed Description

Statement

Reference

Doa^HD/Doa^Dem mutant flies show a mild female to male somatic sex transformation phenotype. Very slight asymmetry is seen and there are a few missing vaginal bristles.

(Fan et al., 2014)

Doa^Dem/Doa^HD testes are filled with early stage germ cells.

Homozygous germline clones result in early germ cell over-proliferation in testes. The overproliferating cells have branched fusomes, indicating that they are spermatogonia.

(Zhao et al., 2013)

In Doa^Dem/Doa^01705b mutant flies, the leg segments distal to the first tarsus often fail to elongate. The femur and claws form normally. Defects are seen in both the mesothoracic and metathoracic tarsi.

The L5 wing vein in Doa^RemγA/Doa^Dem mutant flies fails to attach to the anterior wing margin. Spurs are also seen in the posterior crossvein.

The ovaries of Doa^E786/Doa^Dem mutant flies appear normal, despite asymmetry in the female genital sex combs.

In Doa^Dem/Doa^198-14 mutants where the Doa^Dem allele is from the mother there are no survivors. When the Doa^198-14 allele comes from the mother the flies are viable, but the postalar bristles are duplicated and the scutellar bristles are forked-like.

In Doa^Dem/Doa⁰⁴⁷⁴³ mutants where the Doa^Dem allele is from the mother only rare pupae eclose and weak adults die quickly.The flies exhibit rough eyes, missing bristles and an incomplete L5 wing vein. When the Doa⁰⁴⁷⁴³ allele comes from the mother there are no survivors.

(Kpebe and Rabinow, 2008)

Transheterozygotes with Doa^01705b have rough eyes and duplicated scutellar bristles.

(Yun et al., 2000)

XX ; Doa^unspecified/Doa^Dem adults have subtle intersexual characteristics in sex combs, pigmentation and genitalia. Female-specific vaginal bristles are missing or irregular in most cases. XX ; Doa⁷/Doa^Dem adults have subtle intersexual characteristics.

(Du et al., 1998)

Darkens w^a.

(Rabinow et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Doa^HD/Doa^Dem has abnormal sex-determination phenotype, enhanceable by Srrm1^GE25979

(Fan et al., 2014)

Doa^HD/Doa^Dem has abnormal sex-determination phenotype, enhanceable by Srrm1^B103

(Fan et al., 2014)

Suppressed by

Statement

Reference

Doa^HD/Doa^Dem has male sterile phenotype, suppressible | partially by Hsap\CLK2^UAS.cZa/Scer\GAL4^VP16.bam

(Zhao et al., 2013)

Doa^HD/Doa^Dem has male sterile phenotype, suppressible | partially by Scer\GAL4^VP16.bam/Mmus\Clk2^UAS.cZa

(Zhao et al., 2013)

Suppressor of

Statement

Reference

Doa[+]/Doa^Dem is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Doa^HD/Doa^Dem is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Doa^unspecified/Doa^Dem is a suppressor of abnormal sex-determination | male phenotype of tra^Hsp83.PS

(Du et al., 1998)

NOT Suppressor of

Statement

Reference

Doa^HD/Doa^Dem is a non-suppressor of lethal phenotype of Scer\GAL4^da.PU, Srrm1^GE25979

(Fan et al., 2014)

Other

Statement

Reference

Doa^HD/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has partially lethal - majority die phenotype

(Fan et al., 2014)

Doa^HD/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has short lived phenotype

(Fan et al., 2014)

Doa⁷/Doa^Dem, Srrm1^B103 has abnormal sex-determination phenotype

(Fan et al., 2014)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has visible | dominant phenotype

(Fan et al., 2010)

Doa^Dem, E(gl)3C^unspecified has viable phenotype

(Yun et al., 2000)

Doa^Dem, E(gl)3C^unspecified has fertile phenotype

(Yun et al., 2000)

Doa^Dem, tra^unspecified has abnormal sex-determination | female phenotype

(Du et al., 1998)

Doa^unspecified/Doa^Dem, tra^unspecified has abnormal sex-determination | dominant | female phenotype

(Du et al., 1998)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Doa^HD/Doa^Dem has phenotype, non-enhanceable by Egfr^E1

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by Ras85D^e2F

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by gl²

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by gl^60j

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by rl¹

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by rl^Sem

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-enhanceable by sina¹

(Yun et al., 2000)

Suppressed by

Statement

Reference

Doa^HD/Doa^Dem has spermatogonium | increased number phenotype, suppressible by bam^UASp.GFP/Scer\GAL4^vas.2.6

(Zhao et al., 2013)

Doa^HD/Doa^Dem has spermatogonium | increased number phenotype, suppressible by Hsap\CLK2^C274Y.UAS/Scer\GAL4^VP16.bam

(Zhao et al., 2013)

Doa^HD/Doa^Dem has spermatogonium | increased number phenotype, suppressible by bam^UASp.GFP/Scer\GAL4^VP16.bam

(Zhao et al., 2013)

NOT suppressed by

Statement

Reference

Doa^HD/Doa^Dem has spermatogonium | increased number phenotype, non-suppressible by Scer\GAL4^VP16.bam/Hsap\CLK2^K192A.UAS

(Zhao et al., 2013)

Doa^HD/Doa^Dem has phenotype, non-suppressible by Egfr^E1

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by Ras85D^e2F

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by gl²

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by gl^60j

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by rl¹

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by rl^Sem

(Yun et al., 2000)

Doa^HD/Doa^Dem has phenotype, non-suppressible by sina¹

(Yun et al., 2000)

Enhancer of

Statement

Reference

Doa[+]/Doa^Dem is an enhancer of male genitalia phenotype of Scer\GAL4^esg.PU, Srrm1^GE25979

(Fan et al., 2014)

Doa^HD/Doa^Dem is an enhancer of male genitalia phenotype of Scer\GAL4^esg.PU, Srrm1^GE25979

(Fan et al., 2014)

Suppressor of

Statement

Reference

Doa[+]/Doa^Dem is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Doa^HD/Doa^Dem is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, Srrm1^GE25979

(Fan et al., 2014)

Other

Statement

Reference

Doa[+]/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has female genitalia phenotype

(Fan et al., 2014)

Doa[+]/Doa^Dem, Scer\GAL4^esg.PU, Srrm1^GE25979 has female analia phenotype

(Fan et al., 2014)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has wing phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has eye phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has wing vein | ectopic phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has female genitalia phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has crossvein phenotype

(Fan et al., 2010)

Doa^HD/Doa^Dem, eEF1γ^GE29510 has posterior crossvein | male phenotype

(Fan et al., 2010)

Doa^Dem, tra^unspecified has female genitalia phenotype

(Du et al., 1998)

Doa^Dem, tra^unspecified has leg | female phenotype

(Du et al., 1998)

Doa^Dem, tra^unspecified has abdominal tergite | female phenotype

(Du et al., 1998)

Doa^unspecified/Doa^Dem, tra^unspecified has female genitalia phenotype

(Du et al., 1998)

Doa^unspecified/Doa^Dem, tra^unspecified has leg | female phenotype

(Du et al., 1998)

Doa^unspecified/Doa^Dem, tra^unspecified has abdominal tergite | female phenotype

(Du et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

SRm160^B103 strongly enhances the female to male somatic sex transformation phenotype seen in Doa^HD/Doa^Dem mutant flies. The male genital arch and "claspers" are seen in XX individuals.

SRm160^B103 produces a female to male somatic sex transformation phenotype in Doa^HD/Doa⁷ mutant flies. The male genital arch and "claspers" are seen in XX individuals.

SRm160^GE25979 strongly enhances the female to male somatic sex transformation phenotype seen in Doa^HD/Doa^Dem mutant flies. The male genital arch and "claspers" are seen in XX individuals.

Doa^HD/Doa^Dem does not suppress the lethality seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^da.PU.

Doa^HD/Doa^Dem almost completely suppresses the smooth, glassy eye seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU. The ommatidial disruption is partially suppressed: the various cell types and layers are all identifiable albeit somewhat perturbed. Although differentiation pigment cells are observed and cone cells secrete a cornea of normal appearance, the pigment and photoreceptor cells fail to elongate to form a normal retina. There is a reduction in the levels of apoptosis (caspase activation).

One copy of Doa^Dem partially suppresses the ommatidial disruption seen when SRm160^GE25979 is expressed under the control of Scer\GAL4^GMR.PU. The various cell types and layers are all identifiable albeit somewhat perturbed. Although differentiation pigment cells are observed and cone cells secrete a cornea of normal appearance, the pigment and photoreceptor cells fail to elongate to form a normal retina. There is a reduction in the levels of apoptosis (caspase activation).

One copy of Doa^Dem enhances the loss of external genitalia phenotype seen in males expressing SRm160^GE25979 under the control of Scer\GAL4^esg.PU. Unlike in Scer\GAL4^esg.PU>SRm160^GE25979 mutant flies alone, a small proportion of females lack external genitals and anal plates.

Very few animals survive when SRm160^GE25979 is expressed under the control of Scer\GAL4^esg.PU in a Doa^HD/Doa^Dem mutant background, but those that do survive completely lack genitalia and analia and die shortly following eclosion.

(Fan et al., 2014)

Expression of bam^{Scer\UAS.P\T.T:Avic\GFP} under the control of either Scer\GAL4^{bam.T:Hsim\VP16} or Scer\GAL4^vas.2.6 promotes the differentiation of spermatogonia to spermatocytes in Doa^Dem/Doa^HD testes.

(Zhao et al., 2013)

Doa^HD Ef1γ^GE29510/Doa^Dem flies show a number of defects, including ectopic wing venation, failure of either of the crossveins to attach (particularly the posterior crossvein in males), aberrantly formed crossveins, occasional eye roughness, curved wings and rarely, partial rotation of the female genitalia.

(Fan et al., 2010)

Doa^Dem in combination with E(gl)3C^unspecified results in flies with normal eyes, wings and bristles. No suppression of w^a is seen in these flies.

(Yun et al., 2000)

Heterozygous tra^unspecified/+ adults have normal sexual differentiation. XX ; tra^unspecified/+ adults that are also carrying Doa^unspecified/Doa^Dem are clearly intersexual with respect to the number or morphology of sex combs. XX; Doa^unspecified tra^unspecified/Doa^Dem + adults have genitalia which have characteristics of both sexes, including male-like clasper bristles and anal pads. Pigmentation of abdominal tergites is intermediate to that of the normal male and female phenotypes. XY flies carrying tra^Hsp83.PS develop phenotypically as nearly perfect females. This female differentiation is substantially reversed if the flies are also carrying Doa^unspecified/Doa^Dem.

(Du et al., 1998)

Xenogenetic Interactions

Statement

Reference

Expression of either Hsap\CLK2^Scer\UAS.cZa or Mmus\Clk2^Scer\UAS.cZa under the control of Scer\GAL4^{bam.T:Hsim\VP16} partially rescues the sterility of Doa^Dem/Doa^HD males.

Expression of Hsap\CLK2^{K192A.Scer\UAS} under the control of Scer\GAL4^{bam.T:Hsim\VP16} is unable to promote the differentiation of spermatogonia in Doa^Dem/Doa^HD testes.

Expression of Hsap\CLK2^{C274Y.Scer\UAS} under the control of Scer\GAL4^{bam.T:Hsim\VP16} is able to promote the differentiation of spermatogonia in Doa^Dem/Doa^HD testes.

(Zhao et al., 2013)

Complementation and Rescue Data

Rescued by

Doa^HD/Doa^Dem is rescued by Scer\GAL4^VP16.bam/Doa^{69.long3'UTR.UAS}

(Zhao et al., 2013)

Doa^HD/Doa^Dem is rescued by Scer\GAL4^VP16.bam/Doa^UAS.PS

(Zhao et al., 2013)

Partially rescued by

Doa^HD/Doa^Dem is partially rescued by Doa^UAS.PK/Scer\GAL4^VP16.bam

(Zhao et al., 2013)

Doa^HD/Doa^Dem is partially rescued by Doa^{139-511.UAS.PS.Tag:FLAG}/Scer\GAL4^VP16.bam

(Zhao et al., 2013)