FlyBase Allele Report: Dmel\path[KG06640]

General Information

Symbol

Dmel\path^KG06640

Species

D. melanogaster

Name

FlyBase ID

FBal0148234

Feature type

allele

Associated gene

Dmel\path

Associated Insertion(s)

P{SUPor-P}path^KG06640

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}path^KG06640

Cytology

Description

A P{SUPor-P} insertion within the first intron of the larger path transcription unit, path-RA.

(Goberdhan et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{SUPor-P}

insertional mutagenesis tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:9,501,590..9,501,590 [+]

(Goberdhan et al., 2005, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Goberdhan et al., 2005)

abnormal developmental rate (with Df(3L)AC1)

(Goberdhan et al., 2005)

abnormal eclosion rhythm

(Goberdhan et al., 2005)

abnormal neuroanatomy | larval stage (with Df(3L)BSC773)

(Lin et al., 2015)

abnormal neuroanatomy | larval stage (with path^dg50)

(Lin et al., 2015)

abnormal neuroanatomy | larval stage | recessive

(Lin et al., 2015)

decreased body size

(Goberdhan et al., 2005)

decreased body size (with Df(3L)AC1)

(Goberdhan et al., 2005)

decreased cell number

(Goberdhan et al., 2005)

decreased cell number | larval stage | somatic clone

(Feng et al., 2020)

decreased cell size

(Goberdhan et al., 2005)

decreased fecundity | female

(Goberdhan et al., 2005)

decreased occurrence of cell division | larval stage | somatic clone

(Feng et al., 2020)

female sterile

(Goberdhan et al., 2005)

female sterile (with Df(3L)AC1)

(Goberdhan et al., 2005)

increased cell size | larval stage | somatic clone

(Feng et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

path^KG06640, path^KG06640/path^dg50 or path^KG06640/Df(3L)BSC773 larvae show severe dendrite growth defects in the large C4da dendritic arbors.

(Lin et al., 2015)

path^KG06640 mutants are developmentally delayed, taking ~3 days longer than wild-type larvae to pupate. The wing discs of path^KG06640 third instar larvae are small when compared with similarly aged wild-type discs, but the extended mutant larval period allows discs to grow to roughly normal size by pupariation. The length of pupal development is not noticeably affected and resulting adults are markedly smaller than wild-type animals, are 40% lighter in weight, have wings with significant reductions in both cell number and size and eyes with small ommatidia. The salivary gland and the fat body are only modestly reduced. Additionally, path^KG06640 females fail to lay eggs.

path^KG06640/Df(3L)AC1 animals are even smaller than path^KG06640 homozygotes. These transheterozygotes are also developmentally delayed and female sterile.

Eyes in which many ommatidia are derived from path^KG06640 clones are indistinguishable in size from normal eyes.

(Goberdhan et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

path^KG06640 has decreased fecundity | female phenotype, suppressible | partially by Tsc1²⁹/Tsc1[+]/gig[+]/gig¹⁹²

(Goberdhan et al., 2005)

path^KG06640 has decreased body size phenotype, suppressible by Scer\GAL4^arm.PS/acs^GS10666

(Goberdhan et al., 2005)

path^KG06640 has decreased cell number phenotype, suppressible by Scer\GAL4^arm.PS/acs^GS10666

(Goberdhan et al., 2005)

path^KG06640 has decreased cell size phenotype, suppressible by Scer\GAL4^arm.PS/acs^GS10666

(Goberdhan et al., 2005)

path^KG06640 has decreased body size phenotype, suppressible by Tsc1²⁹/Tsc1[+]/gig[+]/gig¹⁹²

(Goberdhan et al., 2005)

path^KG06640 has decreased cell number phenotype, suppressible by Tsc1²⁹/Tsc1[+]/gig[+]/gig¹⁹²

(Goberdhan et al., 2005)

path^KG06640 has decreased cell size phenotype, suppressible by Tsc1²⁹/Tsc1[+]/gig[+]/gig¹⁹²

(Goberdhan et al., 2005)

path^KG06640 has abnormal developmental rate phenotype, suppressible by Tsc1²⁹/Tsc1[+]/gig[+]/gig¹⁹²

(Goberdhan et al., 2005)

path^KG06640 has female sterile phenotype, suppressible | partially by Tsc1²⁹/Tsc1[+]/gig[+]/gig¹⁹²

(Goberdhan et al., 2005)

Suppressor of

Statement

Reference

path^KG06640 is a suppressor of lethal | somatic clone | pupal stage phenotype of gig¹⁹²

(Goberdhan et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

path^KG06640 has wing phenotype, suppressible by acs^GS10666

(Goberdhan et al., 2005)

path^KG06640 has wing phenotype, suppressible by Tsc1²⁹/Tsc1[+]/gig[+]/gig¹⁹²

(Goberdhan et al., 2005)

Suppressor of

Statement

Reference

path^KG06640 is a suppressor of eye | somatic clone phenotype of gig¹⁹²

(Goberdhan et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The path^KG06640 reduced weight and small wing phenotypes are significantly rescued by expression of CG1139^GS10666 under the control of Scer\GAL4^arm.PS.

The growth and developmentally delayed phenotypes of path^KG06640 mutants are significantly rescued by the presence of combined heterozygous Tsc1²⁹ and gig¹⁹² mutations. About 10% of rescued females are able to lay a small number of eggs, some of which can develop into adult flies.

When clonal material in the eye is mutant for both gig¹⁹² and path^KG06640 in an otherwise heterozygous animal, the lethal effects of gig¹⁹² are suppressed, and about 10% of mutant animals, both males and females, eclose. Their eyes are smaller and bulge less than gig¹⁹² mutant eyes.

(Goberdhan et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

path^KG06640 is partially rescued by path^UAS.cGa/Scer\GAL4^arm.PS

(Goberdhan et al., 2005)

Comments

Expression of path^Scer\UAS.cGa under the control of the constitutive Scer\GAL4^arm.PS driver significantly rescues the growth, developmental delay and female sterility mutant phenotypes of path^KG06640 homozygotes. Rescued flies are slightly smaller than controls.

(Goberdhan et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

14607

y¹; P{SUPor-P}path^KG06640 ry⁵⁰⁶/TM3, Sb¹ Ser¹

Kyoto

111613

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{SUPor-P}path^KG06640 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin