A 161 bp deletion and 7bp insertion that causes a frameshift after amino acid 919 and truncation of the protein after the addition of 16 out of frame amino acids. Position of mutation on reference sequence inferred by FlyBase curator based on author statement.
eye disc & mitotic cell cycle | ectopic | somatic clone
eye disc & S phase | ectopic | somatic clone
gig192/Rbf15aΔ mutant eye clones are smaller than gig192 mutant eye clones. Adult eyes with gig192/Rbf15aΔ double mutant cones are smaller and display a rough appearance, compared to wild-type eyes. This correlates with an increase in apoptosis in both anterior and posterior (to the morphogenic furrow) clones in double mutant eye discs. This synergistic induction of cell death is also seen in the wing disc.
The presence of a Nc1 background significantly decreases gig192/Rbf15aΔ induced cell death in the posterior (the differentiating part of the eye disc). However Nc1 has little effect on cell death of gig192/Rbf15aΔ clones in the anterior, the proliferating part of the eye disc. These triple mutants exhibit an increase in mutant tissue in the eye as well as their overall size.
Heterozygosity for gig192 substantially suppresses the late eclosion phenotype and the semi-lethality of pic2/picPL12c animals, but the bristle defects caused by pic2/picPL12c are not significantly suppressed by gig192/+.
The induction of autophagy in the fat body of normally fed animals that is caused by expression of Atg1Scer\UAS.cSa under the control of Scer\GAL4hs.PH is significantly reduced if the animals are also carrying gig109/gig192.
gig192 does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; gig192 clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, gig192 does not prevent apoptosis of Df(1)su(s)R194/+ cells in the wing.
The pathKG06640 growth and developmentally delayed phenotypes are significantly rescued by the presence of combined heterozygous Tsc129 and gig192 mutations. About 10% of rescued females are able to lay a small number of eggs, some of which can develop into adult flies.
When clonal material in the eye is mutant for both gig192 and pathKG06640 in an otherwise heterozygous animal, the lethal effects of gig192 are suppressed, and about 10% of mutant animals, both males and females, eclose. Their eyes are smaller and bulge less than gig192 mutant eyes.
The large eye phenotype seen in animals in which the head is homozygous for gig192 (induced using the eyFLP system) is not further enhanced if the heads are also homozygous for both scylEP9.85 and chrb180.
The increase in cell size seen in somatic clones of gig192 in the eye is completely suppressed in double mutant somatic clones with S6kl-1. The decrease in cell size seen in somatic clones of S6kl-1 in the eye is completely suppressed in double mutant somatic clones with gig192.