FlyBase Allele Report: Scer\GAL4[hs.PH]

General Information

Symbol

Scer\GAL4^hs.PH

Species

S. cerevisiae

Name

heat shock construct of Hayashi

FlyBase ID

FBal0063247

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{hsGAL4.H}

Also Known As

Hs-gal4, hsp70-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Halfon et al., 1997)

Progenitor genotype

Carried in construct

P{hsGAL4.H}

Cytology

Description

Hsp70 promoter regulates expression of a GAL4 driver.

(Halfon et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism | ubiquitous

(Dumstrei et al., 2003)

Additional Information

Statement

Reference

ScerGAL4^hs.PH drives ubiquitious expression in response to a heat pulse.

(Dumstrei et al., 2003)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Rbf^UAS.cDa

(Fung et al., 2002)

abnormal mitotic cell cycle | third instar larval stage, with shg^ex.UAS

(Dumstrei et al., 2003)

abnormal neuroanatomy | heat sensitive, with mam^H.UAS

(Yedvobnick et al., 2004)

abnormal neuroanatomy | heat sensitive, with mam^N.UAS

(Yedvobnick et al., 2004)

abnormal size | larval stage, with shg^ex.UAS

(Dumstrei et al., 2003)

decreased cell number | larval stage, with shg^ex.UAS

(Dumstrei et al., 2003)

decreased cell number | larval stage, with Tlk^UAS.cCa

(Carrera et al., 2003)

decreased occurrence of cell division | larval stage, with shg^ex.UAS

(Dumstrei et al., 2003)

increased cell size | larval stage, with Tlk^UAS.cCa

(Carrera et al., 2003)

lethal, with crc^UAS.cHa

(Hewes et al., 2000)

lethal | heat sensitive, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

lethal | heat sensitive, with dpp^UAS.cSa

(Muzzopappa and Wappner, 2005)

visible, with fray^UAS.cLa

(Leiserson et al., 2000)

visible | heat sensitive, with mam^H.UAS

(Yedvobnick et al., 2004)

Phenotype Manifest In

abdomen, with Rbf^UAS.cDa

(Fung et al., 2002)

anterior protocerebral neuromere, with shg^ex.UAS

(Dumstrei et al., 2003)

cell body glial cell, with shg^ex.UAS

(Dumstrei et al., 2003)

dorsal appendage, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

dorsal appendage, with dpp^UAS.cSa

(Muzzopappa and Wappner, 2005)

dorsal appendage | ectopic, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

dorsal appendage | ectopic | heat sensitive, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

dorsal appendage | heat sensitive, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

ectoderm | heat sensitive, with mam^N.UAS

(Yedvobnick et al., 2004)

egg operculum, with dpp^UAS.cSa

(Muzzopappa and Wappner, 2005)

eye | heat sensitive, with mam^H.UAS

(Yedvobnick et al., 2004)

fat body, with Atg1^UAS.cSa

(Scott et al., 2007)

female germline stem cell, with dpp^UAS.cSa

(Muzzopappa and Wappner, 2005)

germarium, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

germline cyst, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

larval brain, with shg^ex.UAS

(Dumstrei et al., 2003)

larval neuron | ectopic | heat sensitive, with mam^N.UAS

(Yedvobnick et al., 2004)

larval RP2 motor neuron | ectopic | heat sensitive, with mam^H.UAS

(Yedvobnick et al., 2004)

macrochaeta | increased number | heat sensitive, with mam^H.UAS

(Yedvobnick et al., 2004)

neuroblast, with shg^ex.UAS

(Dumstrei et al., 2003)

neuropil, with shg^ex.UAS

(Dumstrei et al., 2003)

neuropil glial cell, with shg^ex.UAS

(Dumstrei et al., 2003)

oocyte, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

RP2sib neuron | heat sensitive, with mam^H.UAS

(Yedvobnick et al., 2004)

stalk follicle cell, with dpp^UAS.cRa

(Muzzopappa and Wappner, 2005)

wing | heat sensitive, with mam^H.UAS

(Yedvobnick et al., 2004)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^hs.PH/Rnor\Stk39^UAS.cLa is a suppressor of lethal phenotype of fray^r1

(Leiserson et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

Atg1^UAS.cSa, Scer\GAL4^hs.PH has fat body phenotype, suppressible by gig¹⁰⁹/gig¹⁹²

(Scott et al., 2007)

Suppressor of

Statement

Reference

Scer\GAL4^hs.PH/Rnor\Stk39^UAS.cLa is a suppressor of larval peripheral nervous system phenotype of fray^r1

(Leiserson et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^hs.PH/mRpL12^UASp.EGFP rescues mRpL12¹⁰⁵³⁴

(Frei et al., 2005)

Scer\GAL4^hs.PH/mam^N.UAS rescues mam⁴⁴

(Yedvobnick et al., 2004)

Scer\GAL4^hs.PH/fray^UAS.cLa rescues fray^r1

(Leiserson et al., 2000)

Partially rescues

Scer\GAL4^hs.PH/Atg1^UAS.cSa partially rescues Atg1^Δ3D

(Scott et al., 2007)

Scer\GAL4^hs.PH/Atg1^K38Q.UAS partially rescues Atg1^Δ3D

(Scott et al., 2007)

Fails to rescue

Scer\GAL4^hs.PH/mam^N.UAS fails to rescue mam⁹

(Yedvobnick et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

By focusing a laser beam to cause a localised heat shock a single gene, in a single cell of choice, at the time point of choice, can be expressed.

(Halfon et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

GAL4^hs.PH

Scer\GAL4^hs.PH

Name Synonyms

heat shock construct of Hayashi

(Halfon et al., 1997)

Secondary FlyBase IDs

References (15)

Report Sections