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General Information
Symbol
Dmel\mamH.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0102514
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-MamH
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A truncated mam open reading frame, ending at nucleotide 1489 (amino acid residue 245), is driven under the control of UASt sequences. Sequences located carboxy to the basic cluster are removed, including both acidic charge clusters.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of mamH.Scer\UAS under the control of Scer\GAL4bbg-C96 results in a 100% penetrant wing nicking phenotype.

The number of cap cells and germline stem cells in the ovaries of 2 day old females expressing mamH.Scer\UAS under the control of Scer\GAL4C587 is significantly reduced compared to wild type.

Expression of mamH.Scer\UAS under the control of Scer\GAL4C96 produces nicks around the wing margin with 100% penetrance (whereas mamH.Scer\UAS heterozygotes do not exhibit any wing phenotypes). This phenotype is partially suppressed in flies also heterozygous for mamEP2022.A.

Expression of mamH.Scer\UAS under the control of Scer\GAL4309 elicits an increase in the number of bristles at the site of the posterior notopleural macrochaete (whereas mamH.Scer\UAS heterozygotes do not exhibit any bristle phenotypes at this site). This phenotype is partially suppressed in flies also heterozygous for mamEP2022.A.

mamH.Scer\UAS; Scer\GAL41151 pupae have normal numbers of dorso-ventral indirect flight muscle founder cells.

Expression of mamH.Scer\UAS under the control of Scer\GAL4hs.PH between 2 and 6 hours of development results in a neurogenic phenotype. Expression of mamH.Scer\UAS under the control of Scer\GAL4hs.PH between 6 and 8 hours of development results in the GMC-1 dividing symmetrically into two RP2 neurons in about 10% of hemisegments. Expression of mamH.Scer\UAS under the control of Scer\GAL4hs.PH during the third larval instar results in duplications of the macrochaetae, scarring of the eye and severe loss of the wing blade.

Shows a low level of scutellar bristle duplications even without a Scer\GAL4 driver. When driven by Scer\GAL4309 perturbations are observed in bristle number predominantly at scutellar and notopleural sites, duplications of macrochaetae are seen at most scutellar sites and posterior notopleural sites. The macrochaetae multiplication phenotype is enhanced by the addition of mam1. When mamH.Scer\UAS is driven by Scer\GAL4C96 wing nicks are seen and there is a loss of wing margin bristles. The addition of mam1 produces a stronger wing nicking phenotype that is incompletely penetrant.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The wing nicking phenotype caused by expression of mamH.Scer\UAS under the control of Scer\GAL4bbg-C96 is suppressed if the flies are also carrying one of taraEP-425, Lk6EP-573, emcD or Atg1EP-1202 or are co-expressing either wdbEP-1037 or emcEP-1561.

The wing nicking phenotype caused by expression of mamH.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also carrying one of Pabp2EP-558, EcREP-939, lolaEP-1000, lolaEP-1538, Lk62, or pumEP-1646 or are co-expressing lilliEP-1630.

Co-expression of Tudor-SNEP-593 has no effect on the wing nicking phenotype caused by expression of mamH.Scer\UAS under the control of Scer\GAL4bbg-C96.

The wing nicking phenotype caused by expression of Rbf280.Scer\UAS under the control of Scer\GAL4bbg-C96 is enhanced if the flies are also co-expressing mamH.Scer\UAS.

The wing margin nicking phenotype produced by expression of mamH.Scer\UAS under the control of Scer\GAL4C96 is enhanced in flies also heterozygous for RpL13AEP3614.A.

The wing margin nicking phenotype produced by expression of mamH.Scer\UAS under the control of Scer\GAL4C96 is slightly enhanced in flies also heterozygous for DlEP3816.A, though the phenotypic effects are largely additive.

The increase in bristle number at the site of the posterior notopleural macrochaete produced by expression of mamH.Scer\UAS under the control of Scer\GAL4309 is enhanced in flies also heterozygous for DlEP3816.A.

The increase in bristle number at the site of the posterior notopleural macrochaete produced by expression of mamH.Scer\UAS under the control of Scer\GAL4309 is slightly suppressed in flies also heterozygous for RpL13AEP3614.A as fewer bristles are evident in some cases.

The addition of Df(1)N-54l9 to flies expressing mamH.Scer\UAS under the control of Scer\GAL4309 produces lethality.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
mamH.Scer\UAS
mamH.UAS
Name Synonyms
Secondary FlyBase IDs
    References (10)