FlyBase Allele Report: Dmel\mTor[ey.Tag:FLAG]

General Information

Symbol

Dmel\mTor^ey.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0217428

Feature type

allele

Associated gene

Dmel\mTor

Associated Insertion(s)

Carried in Construct

P{ey-TOR.FLAG}

Key Links

Transgenic product class

wild_type

(Hennig et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hennig et al., 2006)

Mutagen

in vitro construct

(Hennig et al., 2006)

Progenitor genotype

Carried in construct

P{ey-TOR.FLAG}

Cytology

Description

Four copies of the eye-specific enhancer fragment from the ey gene and a noninducible heat-shock promoter regulate expression of Tag:FLAG-tagged Tor coding sequence.

(Hennig et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

mTor

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death

(Hennig et al., 2006)

Phenotype Manifest In

eye

(Hennig et al., 2006)

eye-antennal disc primordium

(Hennig et al., 2006)

Detailed Description

Statement

Reference

Tor^{ey.hs.T:Zzzz\FLAG} expression leads to an overall reduction in the size of the adult eye. Ommatidial patterning is disorganised, with some ommatidia appearing fused or pitted.

Tor^{ey.hs.T:Zzzz\FLAG} eye imaginal discs experience a delay in morphogenetic furrow progression and missing and disorganised photoreceptor cells, as well as an increased level of cell death.

(Hennig et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mTor^ey.Tag:FLAG has increased cell death phenotype, enhanceable by shi^K44A.UAS/Scer\GAL4^ey.PH

(Hennig et al., 2006)

mTor^ey.Tag:FLAG has increased cell death phenotype, enhanceable by Tsc1²⁹/Tsc1[+]

(Hennig et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

mTor^ey.Tag:FLAG has eye phenotype, enhanceable by Hsc70-4^e3/Hsc70-4[+]

(Hennig et al., 2006)

mTor^ey.Tag:FLAG has eye phenotype, enhanceable by Hsc70-4^e19/Hsc70-4[+]

(Hennig et al., 2006)

mTor^ey.Tag:FLAG has eye phenotype, enhanceable by shi^K44A.UAS/Scer\GAL4^ey.PH

(Hennig et al., 2006)

mTor^ey.Tag:FLAG has eye-antennal disc primordium phenotype, enhanceable by shi^K44A.UAS/Scer\GAL4^ey.PH

(Hennig et al., 2006)

mTor^ey.Tag:FLAG has eye phenotype, enhanceable by Tsc1²⁹/Tsc1[+]

(Hennig et al., 2006)

Suppressed by

Statement

Reference

Hsc70-4^e19/Hsc70-4[+], mTor^ey.Tag:FLAG has eye phenotype, suppressible by Hsc70-4^+t14

(Hennig et al., 2006)

Hsc70-4^e3/Hsc70-4[+], mTor^ey.Tag:FLAG has eye phenotype, suppressible by Hsc70-4^+t14

(Hennig et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the Tor^{ey.hs.T:Zzzz\FLAG} phenotype is strongly enhanced by a heterozygous Tsc1²⁹ background and is suppressed by addition of rapamycin to the media.

Expression of shi^{K44A.Scer\UAS} (under the control of Scer\GAL4^ey.PH) strongly enhances the Tor^{ey.hs.T:Zzzz\FLAG} phenotype.

Heterozygous Hsc70-4^e3 mutants cause a further reduction in eye size of Tor^{ey.hs.T:Zzzz\FLAG} mutants.

Heterozygous Hsc70-4^e19 mutants cause a further reduction in eye size of Tor^{ey.hs.T:Zzzz\FLAG} mutants.

The presence of Hsc70-4^+t14 suppresses enhancement of the Tor^{ey.hs.T:Zzzz\FLAG} eye phenotype by Hsc70-4^e3 or Hsc70-4^e19.

(Hennig et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tor^ey.Tag:FLAG

Tor^{ey.hs.T:Zzzz\FLAG}

mTor^ey.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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