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General Information
Symbol
Dmel\ThorLL.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0147362
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a mutated form of Thor (carries the amino acid replacements M59L and K60L).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4VGlut-OK371-mediated expression in motor neurons results in muscle 24 denervation in the vast majority of larvae.

Scer\GAL4ppk.PU-mediated expression in class IV multidendritic sensory neurons induces severe morphology defects, with reduction of dendritic coverage by around 40%.

Expression of ThorLL.Scer\UAS under the control of Scer\GAL4elav-C155 increases bouton size compared to controls.

Overexpression of ThorLL.Scer\UAS in muscles under the control of Scer\GAL4Mhc.PW significantly preserves muscle protein homeostasis, while the muscles of wild-type animals display increased accumulation of protein aggregates. Compared to syngenic controls, the age-related accumulation of protein aggregates in retinas, brains and the peripheral fat body of the abdomen is also decreased in flies overexpressing ThorLL.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Muscle functionality gradually decreases in wild-type aging flies, resulting in impaired climbing and flight ability. Overexpression of ThorLL.Scer\UAS under the control of Scer\GAL4Mhc.PW significantly preserves muscle strength during aging.

Overexpression of ThorLL.Scer\UAS under the control of Scer\GAL4Mhc.PW in muscles is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

Food intake of flies is decreased in response to overexpression of ThorLL.Scer\UAS in muscles via Scer\GAL4Mhc.PW.

The body weight of adult flies overexpressing ThorLL.Scer\UAS under the control of Scer\GAL4Mhc.PW does not significantly differ from that of syngenic controls.

There is a significant decrease in the hemolymph glucose concentration compared with syngenic control in flies overexpressing ThorLL.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Overexpression of ThorLL.Scer\UAS under the control of Scer\GAL4arm.PS extends the mean life-span of females by 14% on rich food, but does not seem to affect males. In contrast, under dietary restriction, there is no lifespan extension, beyond the effect of dietary restriction alone.

When expression is driven in the wing, by Scer\GAL4Bx-MS1096, wing size is reduced, though pattern is unaffected. The effect on size is evident in the wing disc. The reduction in size is caused by reduction in cell number. When expression is driven in the eye disc, via Scer\GAL4GMR.PF, the resulting eyes, though normally patterned, have ommatidia reduced in size (not number) compared to those of control eyes. When expression is driven by Scer\GAL4Act5C.PP, in clones in the larval fat body, the size of the fat body cells is reduced.

Expression of ThorLL.Scer\UAS under the control of Scer\GAL4Bx-MS1096 using the P{UAS-Thor.LL}w transgene causes a marked reduction in wing size (11.2 +/- 3.4%) without affecting cell number. Wing discs in these animals are indistinguishable from control discs. Expression of ThorLL.Scer\UAS under the control of Scer\GAL4Bx-MS1096 using the P{UAS-Thor.LL}s transgene causes a marked reduction in wing size (37.1 +/- 2.7%) which is partly due to a decrease in cell number (19.0 +/- 4.3%). Wing discs in these animals are 52% smaller than normal and contain many apoptotic cells. The flies take 1-2 days longer to eclose than normal. Clones in the wing disc expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP using the P{UAS-Thor.LL}w transgene have fewer cells than wild-type clones. Few clones in the wing disc expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP using the P{UAS-Thor.LL}s transgene are recovered and they usually contain 1-2 cells. Clones in the wing disc expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP are smaller in size than wild type. Clones in the fat body expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP using the P{UAS-Thor.LL}s transgene are 45-70% smaller than neighbouring wild-type cells. Clones in the fat body expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP using the P{UAS-Thor.LL}s transgene are recovered in the posterior of the eye disc but are absent from the anterior of the eye disc and the antennal disc. Expression of ThorLL.Scer\UAS under the control of Scer\GAL4dpp.blk1 using the P{UAS-Thor.LL}s transgene results in reduction in size of the region encompassed by wing veins 3 and 4, the anterior crossvein and the wing margin.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference

Scer\GAL4Mhc.PW, ThorLL.UAS has long lived phenotype, non-suppressible by foxo21/foxo[+]

Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of LamCUAS.cSa and ThorLL.UAS under the control of Scer\GAL4fln.IFM under the control of Scer\GAL4fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

Expression of ThorLL.Scer\UAS does not suppress the enlarged bouton phenotype seen when Vap-33AP58S.cRa.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

Expression of ThorLL.Scer\UAS suppresses the small bouton phenotype seen when Vap-33AScer\UAS.cRa is expressed under the control of Scer\GAL4elav-C155.

Overexpression of ThorLL.Scer\UAS under the control of Scer\GAL4Mhc.PW in a heterozygous foxo21 mutant genetic background is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.

Co-expression of BacA\p35Scer\UAS.cHa partially rescues the reduced size of wing discs in animals expressing ThorLL.Scer\UAS under the control of Scer\GAL4Bx-MS1096 using the P{UAS-Thor.LL}s transgene. The number of cells in clones in the wing disc expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP using the P{UAS-Thor.LL}w is not affected by co-expression of BacA\p35Scer\UAS.cHa. Co-expression of BacA\p35Scer\UAS.cHa greatly increases the number of clones recovered in the wing disc expressing ThorLL.Scer\UAS under the control of Scer\GAL4Act5C.PP using the P{UAS-Thor.LL}s transgene, but only marginally increases the number of cells per clone. The region of the wing encompassed by wing veins 3 and 4, the anterior crossvein and the wing margin is reduced in size when ThorLL.Scer\UAS is expressed under the control of Scer\GAL4dpp.blk1 using the P{UAS-Thor.LL}s transgene in a Df(2L)Pi3K21B-A/+; P{U2af38.gR10} background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
ThorLL.Scer\UAS
ThorLL.UAS
Name Synonyms
Secondary FlyBase IDs
    References (14)