UAS sequences drive expression of Hsap\APLP1.
Expression of Hsap\APLP1Scer\UAS.cMa driven along the anterior/posterior compartment boundary in third instar larval wing discs by Scer\GAL4ptc-559.1 or Scer\GAL4dpp.PU leads to increased cell death in this region, along with a loss of anterior crossvein phenotype in adult fly wings, compared to controls. Expression of Hsap\APLP1Scer\UAS.cMa driven in the posterior compartment of third instar larval wing discs by Scer\GAL4en.PU leads to increased cell death in this region, along with a loss of anterior crossvein phenotype in adult fly wings, compared to controls. Expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4sd-SG29.1 leads to extensive cell death in the wing pouch of third instar larvae and produces a blistered wing phenotype in adults. Expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4pnr-MD237 induces cell death in the notum tips of wing discs and leads to a small scutellum phenotype in flies, compared to controls. Expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4elav.PU induces cell death in the ventral nerve cord of third instar larvae. Expression of Hsap\APLP1Scer\UAS.cMa driven in eye discs of third instar larvae by Scer\GAL4GMR.PU induces cell death posterior to the morphogenetic furrow.
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has increased cell death | third instar larval stage phenotype, suppressible by BacA\p35UAS.cUa, Scer\GAL4ptc-559.1
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has increased cell death | third instar larval stage phenotype, suppressible | partially by foxo21/foxo[+]
Hsap\APLP1UAS.cMa, Scer\GAL4sd-SG29.1 has visible phenotype, suppressible by foxo[+]/foxoΔ94
Hsap\APLP1UAS.cMa, Scer\GAL4sd-SG29.1 has visible phenotype, suppressible by foxo21/foxo[+]
Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237 has increased cell death | third instar larval stage phenotype, suppressible by foxoKK108590, Scer\GAL4pnr-MD237
Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237 has visible phenotype, suppressible by foxoKK108590, Scer\GAL4pnr-MD237
Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237 has visible phenotype, suppressible by foxo[+]/foxoΔ94
Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237 has visible phenotype, suppressible by foxo21/foxo[+]
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has increased cell death | third instar larval stage phenotype, suppressible by Diap1UAS.cUa, Scer\GAL4ptc-559.1
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has increased cell death | third instar larval stage phenotype, suppressible | partially by foxoKK108590, Scer\GAL4ptc-559.1
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has visible phenotype, suppressible | partially by foxoKK108590, Scer\GAL4ptc-559.1
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has increased cell death | third instar larval stage phenotype, suppressible | partially by foxo[+]/foxoΔ94
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by BacA\p35UAS.cUa, Scer\GAL4ptc-559.1
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by foxo21/foxo[+]
Hsap\APLP1UAS.cMa, Scer\GAL4sd-SG29.1 has wing blade | adult stage phenotype, suppressible by foxo[+]/foxoΔ94
Hsap\APLP1UAS.cMa, Scer\GAL4sd-SG29.1 has wing blade | adult stage phenotype, suppressible by foxo21/foxo[+]
Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237 has presumptive scutellum | third instar larval stage phenotype, suppressible by foxoKK108590, Scer\GAL4pnr-MD237
Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237 has scutellum | adult stage phenotype, suppressible by foxoKK108590, Scer\GAL4pnr-MD237
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible by Diap1UAS.cUa, Scer\GAL4ptc-559.1
Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237 has scutellum | adult stage phenotype, suppressible by foxo[+]/foxoΔ94
Hsap\APLP1UAS.cMa, Scer\GAL4pnr-MD237 has scutellum | adult stage phenotype, suppressible by foxo21/foxo[+]
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by foxoKK108590, Scer\GAL4ptc-559.1
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has anterior crossvein | adult stage phenotype, suppressible | partially by foxoKK108590, Scer\GAL4ptc-559.1
Hsap\APLP1UAS.cMa, Scer\GAL4ptc-559.1 has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, suppressible | partially by foxo[+]/foxoΔ94
Co-expression of BacA\p35Scer\UAS.cUa or Diap1Scer\UAS.cUa suppresses the increased cell death induced by expression of Hsap\APLP1Scer\UAS.cMa driven along the anterior/posterior compartment boundary in third instar larval wing discs by Scer\GAL4ptc-559.1.
Presence of foxoΔ94/+ or foxo21/+ significantly partially suppresses the increased cell death induced by expression of Hsap\APLP1Scer\UAS.cMa driven along the anterior/posterior compartment boundary in third instar larval wing discs by Scer\GAL4ptc-559.1. Co-expression of foxoKK108590 significantly partially suppresses the increased cell death in third instar larval wing discs and the loss of the anterior crossvein in adult wings induced by expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4ptc-559.1. Presence of foxoΔ94/+ or foxo21/+ suppresses the blistered wing phenotype in flies with expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4sd-SG29.1.
Co-expression of foxoKK108590 significantly suppresses the increased cell death in the notum tips of third instar larval wing discs and the small scutellum phenotype in adults induced by expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4pnr-MD237. Presence of foxoΔ94/+ or foxo21/+ suppresses the small scutellum phenotype seen with expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4pnr-MD237.