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General Information
Symbol
Dmel\futschK68
Species
D. melanogaster
Name
FlyBase ID
FBal0118232
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    ameliorates  Troyer syndrome
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    futschK68 larval class IV da neurons show a significant increase in the number of dendrite tips, as compared to controls.

    futschK68/futschK68 mutant larval neuromuscular junctions do not exhibit aberrant microtubule accumulation; but do show a small but significant decrease in the diameter of the microtubule core filament; mutants also show a significant reduction in synaptic bouton number; and there is a non-significant reduction in evoked postsynaptic potential (EPSP) or mEPSP amplitude and quantal content, as compared to controls. Type 1b axons innervating NMJs do not exhibit microtubule organization defects in futschK68/futschK68 mutant larvae.

    20 day old adult heterozygotes show increased vacuolisation in the brain compared to controls.

    Mutant flies show mild but significant defects in the auditory response to sound compared to wild type.

    There is a decrease in the number of synaptic boutons at the neuromuscular junction in mutant third instar larvae.

    futschK68 mutants display a 20% decrease in the number of boutons in larval neuromuscular junctions and a decrease in the number of "satellite" boutons, defined as boutons that are present in the axon of a branch or that bud off from neuronal connections between two boutons.

    Mutant animals exhibit increased microtubule fragmentation in neuromuscular junction synaptic boutons.

    Neuronal development in the embryo proceeds normally. In larvae a reduced number of synaptic boutons are seen.

    In futschK68 homozygotes, synaptic microtubules no longer form a filamentous cytoskeletal shaft that runs through the nerve terminal, and microtubule loop formation is absent. There is also a reduction in bouton number and an increase in bouton size (bouton sizes increase over two-fold). Bouton number is reduced from an average of 64.8 boutons at muscle 4 in wild-type to 37.9 in mutants. Also mutant nerve-terminals at muscle 4 rarely have branch-points. No change in muscle size is seen in these mutants.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference
    Suppressed by
    NOT suppressed by
    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Other
    Phenotype Manifest In
    Enhanced by
    Suppressed by
    NOT suppressed by
    Suppressor of
    NOT Suppressor of
    Other
    Statement
    Reference

    wrd104, futschK68/futsch[+] has bouton & neuromuscular junction | larval stage phenotype

    PP2A-B'[+]/wrd104, futschK68 has bouton & neuromuscular junction | larval stage phenotype

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1HMS00405 under the control of Scer\GAL4ppk.PG is not significantly modified by combination with a single copy of futschK68.

    futschK68/futschK68 suppresses the aberrant microtubule aggregations in Ank2XLΔ/Ank2XLΔ mutant larval neuromuscular junctions and the axons innervating NMJs, but Ank2XLΔ/Ank2XLΔ enhances the decrease in the diameter of the microtubule core filament seen in futschK68/futschK68 mutants; futschK68/futschK68, Ank2XLΔ/Ank2XLΔ double mutants show perturbation of the area of individual synaptic boutons, either dramatically increased or reduced to small varicosities; double mutants exhibit a dramatic reduction in evoked postsynaptic potential (EPSP) amplitude, quantal content, and propagation time from stimulation artefact to the start of EPSP, but no changes in mEPSP amplitude; axon diameter of individual muscle 4 motoneurons is significantly reduced; and double mutants exhibit a significant increase in the fraction of stationary mitochondria and a decrease in anterograde transport velocities in axons, as compared to controls.

    Expression of Ank2XL-ΔR, Ank2XL-5xR or Ank2XL-20xR, but not Ank2XL-Δall or Ank2XL-ΔC, in a Ank2f00518/Ank2f00518 mutant background partially rescues the decreased microtubule core filament area seen in futschK68/futschK68 mutant larval neuromuscular junctions.

    futschK68/futschK68 fails to suppress the defective synaptic stability of Ank2f00518/Ank2f00518 mutant larval neuromuscular junctions.

    The brain vacuolisation seen in 20 day old spartin1/Df(3R)110 and futschK68/+ adults is suppressed in futschK68/+ ; spartin1/Df(3R)110 double mutant flies.

    A futschK68/+ background suppresses the increase in type 1b bouton area observed in foxo21 homozygotes. Furthermore, this background dominantly suppresses the elevated number of microtubule loops present in foxo21 mutants.

    A futschK68/+ background suppresses the FM 1-43 loading defects found in foxo21 mutant synapses.

    The synaptic overgrowth phenotype at the neuromuscular junction seen in third instar larvae lacking Cdk5 function (Df(2R)Exel9026 homozygotes with all deleted gene functions except Cdk5 restored using P{Flo-1wt.Cdk5Δ.CG30466wt.CG8204wt}) is not altered if the larvae are also homozygous or futschK68.

    The neuromuscular junction of futschK68/+; PP2A-B'104/+ larvae have significantly larger boutons than wild-type. In contrast, either heterozygote alone has normal sized boutons.

    Expression of sggA81T.Scer\UAS under the control of Scer\GAL4OK6, in a futschK68 background shows the futschK68 phenotype in which larval neuromuscular synaptic bouton number is decreased and not the opposite sggA81T.Scer\UAS overexpression phenotype.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    Expression of futschN-Term.Scer\UAS or futschC-Term.Scer\UAS separately under the control of Scer\GAL4da.G32 fails to rescue the reduction in bouton number at the neuromuscular junction seen in futschK68 third instar larvae.

    Expression of futschN-Term.Scer\UAS and futschC-Term.Scer\UAS together under the control of Scer\GAL4da.G32 partially the reduction in bouton number at the neuromuscular junction seen in futschK68 third instar larvae.

    Expression of either futschN+C-Term.Scer\UAS or futschNPC-Term.Scer\UAS separately under the control of Scer\GAL4da.G32 partially rescues the reduction in bouton number at the neuromuscular junction seen in futschK68 third instar larvae.

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    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    References (19)