Larval muscle 4 neuromuscular junctions in Ank2XLΔ homozygotes display a significant decrease in the number of synaptic boutons, in parallel with bouton fusion and pronounced microtubule accumulation in the nerve terminal, as compared to controls; heterozygotes, however, do not show significant changes in synaptic bouton number, as compared to controls.
Ank2f00518/Ank2f00518 mutant larvae exhibit lethality, and neuromuscular junctions exhibit defects in synaptic morphology and stability, and defects in microtubule organization.
Expression of Ank2XL-Δall or Ank2XL-ΔC in a Ank2f00518/Ank2f00518 background in larvae results in neuromuscular junctions exhibiting defective microtubule organization and synapse morphology, and the type 1b axons innervating NMJs exhibit aberrant axonal microtubule accumulations, as compared to controls.
Expression of Ank2XL-5xR, Ank2XL-ΔR, or Ank2XL-20xR, in a Ank2f00518/Ank2f00518 background in larvae results in type 1b axons innervating NMJs exhibit aberrant axonal microtubule accumulations, as compared to controls.
Ank2f00518 homozygous mutants exhibit a synaptic retraction phenotype.
Homozygous and Ank2f00518/Df(3L)RM5-2 animals show severe synaptic retractions at 29[o]C of neuromuscular junctions.
Ank2f02001/Ank2f00518 animals die as third instar larvae/early pupae, and they show severe synaptic retractions at the neuromuscular junctions.
Ank2f00518/Df(3L)RM5-2 second instar larvae show defects in synaptic transmission and motoneuron excitability at the NMJ. Defects in in synapse morphology are also seen at the NMJ, with loss of the narrow interbouton regions and loss of discrete, evenly spaced boutons. The presynaptic microtubule cytoskeleton is severely disorganised.