FlyBase Allele Report: Dmel\futsch[N94]

General Information

Symbol

Dmel\futsch^N94

Species

D. melanogaster

Name

FlyBase ID

FBal0118230

Feature type

allele

Associated gene

Dmel\futsch

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Franco et al., 2004)

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X syndrome

modeled by Fmr1^Δ50M

(Zhang et al., 2001)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

In futsch^N94/futsch^N94 embryos the axons of ap-expressing neurons are mildly disorganized, with no impact on the overall organization of the Fas2-immunopositive scaffold.

(Stratmann et al., 2019)

futsch^N94 mutant pupae prune all ddaC dendrites by 16 h after puparium formation (APF), similar to controls.

(Wang et al., 2019)

The third instar larval neuromuscular junctions of futsch^N94 heterozygotes display a significant decrease in the number, and a significant increase in the size, of terminal boutons, as compared to controls.

(Ma et al., 2017)

No defects in microtubule stability are observed in the distal boutons of the futsch^N94 mutant NMJ.

(Godena et al., 2011)

futsch^N94 heterozygotes exhibit wild-type synaptic differentiation.

futsch^N94 mutants exhibit a reduction in synaptic mitochondria.

(Lee et al., 2010)

There is a decrease in the number of synaptic boutons at the neuromuscular junction in mutant third instar larvae.

(Gogel et al., 2006)

futsch^N94 mutants display a 20% decrease in the number of boutons in larval neuromuscular junctions and a decrease in the number of "satellite" boutons, defined as boutons that are present in the axon of a branch or that bud off from neuronal connections between two boutons.

(Franco et al., 2004)

Mutants show no circadian defect detectable with actograms.

(Dockendorff et al., 2002)

Phenotype is similar to that of Fmr1 gain of function mutants - reduced neuromuscular junction growth with decreased synaptic area, branching and bouton number. Neurotransmission at the NMJ is elevated, and in the photoreceptor is depressed.

(Zhang et al., 2001)

In futsch^N94 homozygotes, synaptic microtubules no longer form a filamentous cytoskeletal shaft that runs through the nerve terminal, and microtubule loop formation is absent. There is also a reduction in bouton number and an increase in bouton size (bouton sizes increase over two-fold). Bouton number is reduced from an average of 64.8 boutons at muscle 4 in wild-type to 40.8 in mutants. Also mutant nerve-terminals at muscle 4 rarely have branch-points. In futsch^N94 heterozygotes, a small but statistically significant reduction of numbers of boutons (about 51.2 at muscle 4). No change in muscle size is seen in these mutants.

(Roos et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

futsch^N94 has abnormal neuroanatomy | dominant phenotype, non-enhanceable by NaPi-III^21-4/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has abnormal size | dominant phenotype, non-enhanceable by NaPi-III^21-4/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has abnormal neuroanatomy | dominant phenotype, non-enhanceable by NaPi-III^15-1/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has abnormal size | dominant phenotype, non-enhanceable by NaPi-III^15-1/NaPi-III[+]

(Ma et al., 2017)

Suppressed by

Statement

Reference

futsch^N94 has abnormal neuroanatomy phenotype, suppressible by Fmr1^Δ50M

(Zhang et al., 2001)

futsch^N94 has abnormal neurophysiology phenotype, suppressible by Fmr1^Δ50M

(Zhang et al., 2001)

Enhancer of

Statement

Reference

futsch^N94/futsch[+] is an enhancer of abnormal neuroanatomy | dominant phenotype of NaPi-III^21-4

(Ma et al., 2017)

futsch^N94/futsch[+] is an enhancer of abnormal size | dominant phenotype of NaPi-III^21-4

(Ma et al., 2017)

futsch^N94/futsch[+] is an enhancer of abnormal neuroanatomy | dominant phenotype of NaPi-III^15-1

(Ma et al., 2017)

futsch^N94/futsch[+] is an enhancer of abnormal size | dominant phenotype of NaPi-III^15-1

(Ma et al., 2017)

NOT Enhancer of

Statement

Reference

futsch^N94 is a non-enhancer of semi-sterile phenotype of Fmr1^Δ50M

(Zhang et al., 2004)

Suppressor of

Statement

Reference

futsch^N94/futsch[+] is a suppressor of abnormal neuroanatomy phenotype of NFAT^EP19579

(Freeman et al., 2011)

futsch^N94/futsch[+] is a suppressor of abnormal neuroanatomy phenotype of NFAT^EP1508

(Freeman et al., 2011)

futsch^N94/futsch[+] is a suppressor of abnormal neuroanatomy phenotype of Lrrk^e03680

(Lee et al., 2010)

futsch^N94 is a suppressor of abnormal neurophysiology phenotype of Fmr1^Δ50M

(Zhang et al., 2001)

futsch^N94 is a suppressor of abnormal neuroanatomy phenotype of Fmr1^Δ50M

(Zhang et al., 2001)

NOT Suppressor of

Statement

Reference

futsch^N94 is a non-suppressor of semi-sterile phenotype of Fmr1^Δ50M

(Zhang et al., 2004)

futsch^N94 is a non-suppressor of abnormal locomotor rhythm phenotype of Fmr1³

(Dockendorff et al., 2002)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

futsch^N94 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by NaPi-III^21-4/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has embryonic/larval motor neuron | third instar larval stage phenotype, non-enhanceable by NaPi-III^21-4/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has terminal bouton | third instar larval stage phenotype, non-enhanceable by NaPi-III^21-4/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by NaPi-III^15-1/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has embryonic/larval motor neuron | third instar larval stage phenotype, non-enhanceable by NaPi-III^15-1/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has terminal bouton | third instar larval stage phenotype, non-enhanceable by NaPi-III^15-1/NaPi-III[+]

(Ma et al., 2017)

futsch^N94 has synapse phenotype, non-enhanceable by Scer\GAL4^how-24B/flexin^EP

(Roos et al., 2000)

Suppressed by

Statement

Reference

futsch^N94 has bouton phenotype, suppressible by Fmr1^Δ50M

(Zhang et al., 2001)

futsch^N94 has neuromuscular junction phenotype, suppressible by Fmr1^Δ50M

(Zhang et al., 2001)

futsch^N94 has synapse phenotype, suppressible by Fmr1^Δ50M

(Zhang et al., 2001)

NOT suppressed by

Statement

Reference

futsch^N94 has NMJ bouton | third instar larval stage | decreased number phenotype, non-suppressible by CanA1^{Δ.UAS.Tag:HA}/Scer\GAL4^Toll-6-D42

(Wong et al., 2014)

futsch^N94 has embryonic/larval hypodermal muscle cell | third instar larval stage phenotype, non-suppressible by CanA1^{Δ.UAS.Tag:HA}/Scer\GAL4^Toll-6-D42

(Wong et al., 2014)

futsch^N94 has bouton phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/sgg^A81T.UAS

(Franco et al., 2004)

futsch^N94 has synapse phenotype, non-suppressible by Scer\GAL4^how-24B/flexin^EP

(Roos et al., 2000)

Enhancer of

Statement

Reference

futsch^N94/futsch[+] is an enhancer of embryonic/larval motor neuron | third instar larval stage phenotype of NaPi-III^15-1

(Ma et al., 2017)

futsch^N94/futsch[+] is an enhancer of terminal bouton | third instar larval stage phenotype of NaPi-III^15-1

(Ma et al., 2017)

futsch^N94/futsch[+] is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of NaPi-III^21-4

(Ma et al., 2017)

futsch^N94/futsch[+] is an enhancer of embryonic/larval motor neuron | third instar larval stage phenotype of NaPi-III^21-4

(Ma et al., 2017)

futsch^N94/futsch[+] is an enhancer of terminal bouton | third instar larval stage phenotype of NaPi-III^21-4

(Ma et al., 2017)

futsch^N94/futsch[+] is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of NaPi-III^15-1

(Ma et al., 2017)

Suppressor of

Statement

Reference

futsch^N94/futsch[+] is a suppressor of lipid droplet | increased number | adult stage phenotype of ND-42^ninaE.GD6220

(Goodman et al., 2024)

futsch^N94/futsch[+] is a suppressor of ommatidium | adult stage phenotype of ND-42^ninaE.GD6220

(Goodman et al., 2024)

futsch^N94/futsch[+] is a suppressor of microtubule phenotype of foxo²¹

(Nechipurenko and Broihier, 2012)

futsch^N94/futsch[+] is a suppressor of synapse phenotype of NFAT^EP1508, Scer\GAL4^Toll-6-D42

(Freeman et al., 2011)

futsch^N94/futsch[+] is a suppressor of synapse phenotype of NFAT^EP19579, Scer\GAL4^Toll-6-D42

(Freeman et al., 2011)

futsch^N94/futsch[+] is a suppressor of neuromuscular junction phenotype of Lrrk^e03680

(Lee et al., 2010)

futsch^N94/futsch[+] is a suppressor of NMJ bouton phenotype of Lrrk^e03680

(Lee et al., 2010)

futsch^N94 is a suppressor of bouton | increased number phenotype of Scer\GAL4^RapGAP1-OK6, sgg^A81T.UAS

(Franco et al., 2004)

futsch^N94 is a suppressor of bouton phenotype of Fmr1^Δ50M

(Zhang et al., 2001)

futsch^N94 is a suppressor of neuromuscular junction phenotype of Fmr1^Δ50M

(Zhang et al., 2001)

futsch^N94 is a suppressor of synapse phenotype of Fmr1^Δ50M

(Zhang et al., 2001)

NOT Suppressor of

Statement

Reference

futsch^N94, Scer\GAL4^elav.PU, futsch[+], TBPH^UAS.Tag:FLAG is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of TBPH^Δ23

(Godena et al., 2011)

futsch^N94, Scer\GAL4^elav.PU, futsch[+], TBPH^UAS.Tag:FLAG is a non-suppressor of NMJ bouton | third instar larval stage phenotype of TBPH^Δ23

(Godena et al., 2011)

futsch^N94, Scer\GAL4^elav.PU, futsch[+], TBPH^UAS.Tag:FLAG is a non-suppressor of microtubule | third instar larval stage phenotype of TBPH^Δ23

(Godena et al., 2011)

Other

Statement

Reference

αTub84B^K394R, futsch^N94 has microtubule | larval stage phenotype

(Saunders et al., 2022)

αTub84B^K394R, futsch^N94 has embryonic/larval neuromuscular junction | larval stage phenotype

(Saunders et al., 2022)

αTub84B^K394R, futsch^N94 has type Ib terminal bouton | larval stage phenotype

(Saunders et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number and increased size of terminal boutons in third instar larval neuromuscular junctions of NaPi-III^21-4, futsch^N94 and NaPi-III^15-1, futsch^N94 double heterozygotes is similar to NaPi-III^21-4 single heterozygotes and enhanced compared to futsch^N94 single heterozygotes.

(Ma et al., 2017)

A futsch^N94 background dominantly suppresses the elevated number of microtubule loops present in foxo²¹ mutants.

(Nechipurenko and Broihier, 2012)

A futsch^N94 heterozygous background restores normal synaptic length when NFAT^EP1508 is expressed under the control of Scer\GAL4^D42.

A futsch^N94 heterozygous background restores normal synaptic length when NFAT^EP19579 is expressed under the control of Scer\GAL4^D42.

(Freeman et al., 2011)

One copy of futsch^N94 prevents expression of TBPH^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU from rescuing the reduction in bouton number and terminal branches seen in muscles 6 and 7 of homozygous TBPH^Δ23 third instar larvae. futsch^N94/+ also prevents TBPH^{Scer\UAS.T:Zzzz\FLAG} from restoring microtubule stability in the TBPH^Δ23 mutant flies.

(Godena et al., 2011)

Removal of futsch activity in a heterozygous futsch^N94 background rescues the synaptic overgrowth phenotype found in Lrrk^e03680 heterozygotes.

(Lee et al., 2010)

Expression of sgg^{A81T.Scer\UAS} under the control of Scer\GAL4^OK6, in a futsch^N94 background suppresses the increased larval neuromuscular bouton number phenotype, seen when sgg^{A81T.Scer\UAS} is expressed in a wild-type background. The phenotype is nearer to that seen in futsch^N94 single mutants, although the bouton number is not decreased as much.

(Franco et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

futsch^olk3

(Bettencourt da Cruz et al., 2005)

futsch^olk2

(Bettencourt da Cruz et al., 2005)

futsch^olk1

(Bettencourt da Cruz et al., 2005)

Partially rescued by

futsch^N94 is partially rescued by Scer\GAL4^elav-C155/futsch^Nterm.UAS

(Roos et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8805

y¹ futsch^N94

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

futsch^N94

(Goodman et al., 2024, Saunders et al., 2022, Worpenberg et al., 2021, Vargas et al., 2020, Shi et al., 2019, Stratmann et al., 2019, Wang et al., 2019, Migh et al., 2018, Ma et al., 2017, Wong et al., 2014, Nechipurenko and Broihier, 2012, Freeman et al., 2011, Godena et al., 2011, Yao et al., 2011, Lee et al., 2010, Zou et al., 2008, Gogel et al., 2006, Bettencourt da Cruz et al., 2005, Franco et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (24)

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