FlyBase Allele Report: Dmel\NaPi-III[21-4]

General Information

Symbol

Dmel\NaPi-III^21-4

Species

D. melanogaster

Name

FlyBase ID

FBal0337967

Feature type

allele

Associated gene

Dmel\NaPi-III

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Ma et al., 2017)

Progenitor genotype

Cytology

Description

1bp deletion at nucleotide 536 (A) of the NaPi-III coding sequence, deletion is in codon 179 (revision of data in FBrf0237660).

(Jin, 2018.6.29)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:11,129,749..11,129,749 [+]

Comment:

The 62nd base (in codon V21) of the NaPi-III CDS is deleted in the mutant causing a frameshift (mistakenly reported as base 62 of the cDNA).

(Jin, 2018.6.29)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | dominant

(Ma et al., 2017)

abnormal size | dominant

(Ma et al., 2017)

lethal - all die during embryonic stage (with Df(3L)BSC817)

(Ma et al., 2017)

lethal - all die during embryonic stage (with Df(3L)ED4470)

(Ma et al., 2017)

lethal - all die during embryonic stage (with NaPi-III^15-1)

(Ma et al., 2017)

Phenotype Manifest In

embryonic/larval motor neuron | third instar larval stage

(Ma et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage

(Ma et al., 2017)

terminal bouton | third instar larval stage

(Ma et al., 2017)

Detailed Description

Statement

Reference

NaPi-III^21-4/NaPi-III^15-1, NaPi-III^21-4/Df(3L)ED4470 or NaPi-III^21-4/Df(3L)BSC817 transheterozygotes are embryonic lethal.

The third instar larval neuromuscular junctions of NaPi-III^21-4 heterozygotes display a significant decrease in the number, and a significant increase in the size, of terminal boutons, as compared to controls.

(Ma et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

NaPi-III^21-4 has abnormal neuroanatomy | dominant phenotype, enhanceable by futsch^N94/futsch[+]

(Ma et al., 2017)

NaPi-III^21-4 has abnormal size | dominant phenotype, enhanceable by futsch^N94/futsch[+]

(Ma et al., 2017)

NOT Enhancer of

Statement

Reference

NaPi-III^21-4/NaPi-III[+] is a non-enhancer of abnormal neuroanatomy | dominant phenotype of futsch^N94

(Ma et al., 2017)

NaPi-III^21-4/NaPi-III[+] is a non-enhancer of abnormal size | dominant phenotype of futsch^N94

(Ma et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

NaPi-III^21-4 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, enhanceable by futsch^N94/futsch[+]

(Ma et al., 2017)

NaPi-III^21-4 has embryonic/larval motor neuron | third instar larval stage phenotype, enhanceable by futsch^N94/futsch[+]

(Ma et al., 2017)

NaPi-III^21-4 has terminal bouton | third instar larval stage phenotype, enhanceable by futsch^N94/futsch[+]

(Ma et al., 2017)

NOT Enhancer of

Statement

Reference

NaPi-III^21-4/NaPi-III[+] is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of futsch^N94

(Ma et al., 2017)

NaPi-III^21-4/NaPi-III[+] is a non-enhancer of embryonic/larval motor neuron | third instar larval stage phenotype of futsch^N94

(Ma et al., 2017)

NaPi-III^21-4/NaPi-III[+] is a non-enhancer of terminal bouton | third instar larval stage phenotype of futsch^N94

(Ma et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased number and increased size of terminal boutons in third instar larval neuromuscular junctions of NaPi-III^21-4, futsch^N94 double heterozygotes is similar to NaPi-III^21-4 single heterozygotes and enhanced compared to futsch^N94 single heterozygotes.

(Ma et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

NaPi-III^21-4/NaPi-III^15-1 is rescued by Scer\GAL4^elav.PU/NaPi-III^UAS.GFP

(Ma et al., 2017)

NaPi-III^21-4/NaPi-III^15-1 is rescued by Scer\GAL4^Act.PU/NaPi-III^UAS.GFP

(Ma et al., 2017)

NaPi-III^21-4/Df(3L)ED4470 is rescued by Scer\GAL4^elav.PU/NaPi-III^UAS.GFP

(Ma et al., 2017)