histone deacetylase required for euchromatic and heterochromatic silencing - interacts genetically and physically with members of the Hairy/Deadpan/E(Spl) family of bHLH euchromatic repressors
Gene model reviewed during 5.44
Gene model reviewed during 5.50
There is only one protein coding transcript and one polypeptide associated with this gene
Interacts with the transcriptional repressors hairy (h) and deadpan (dpn); via basic domains. Associates with the Esc/E(z) histone methyltransferase complex. Interacts directly with E(z) and HDAC1/Rpd3.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sirt1 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Sirt1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Renamed from 'Sir2' to 'Sirt1' to match the naming convention of the other D. melanogaster Sirtuin genes (i.e. nomenclature follows that of the mammalian orthologs).
Overexpression of Sir2 between approximately 2-5 fold above normal results in a dose-dependent increase in lifespan. Expression below this range or slightly above it (5-10 fold above normal) inconsistently extends lifespan, while higher levels of expression are detrimental to lifespan.
DNA-protein interactions: genome-wide binding profile assayed for Sir2 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
Homozygosity for a Sir2 mutation has no apparent deleterious effect on viability, developmental rate or sex ratio.
Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex.