FlyBase Allele Report: Dmel\Sirt1[4.5]

General Information

Symbol

Dmel\Sirt1^4.5

Species

D. melanogaster

Name

FlyBase ID

FBal0141417

Feature type

allele

Associated gene

Dmel\Sirt1

Associated Insertion(s)

Carried in Construct

Also Known As

Sir2^4.5, dSir2^4.5

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

P{lacW}k14513

(Newman et al., 2002)

Cytology

Description

Imprecise excision of the P{PZ} element, resulting in a deletion of Sirt1 sequences from -16 to +759 (coding sequences of the adjacent gene, DnaJ-H, are intact).

(Newman et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:13,165,535..13,166,310 [+]

Comment:

A deletion resulting from the imprecise excision of P{PZ}Sirt1^05327a, removes sequences from -16 to +759 with respect to the Sirt1 transcription start. The start site was mapped to a base that corresponds to the 5' end of EST GB:LD07439.

(Newman et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of type 2 diabetes mellitus

CEA with Sirt1^2A-7-11

(Palu and Thummel, 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile (with Sirt1^5.26)

(Newman et al., 2002)

short lived | progressive | calorie restriction conditional (with Sirt1^2A-7-11)

(Palu and Thummel, 2016)

suppressor of variegation (with Sirt1^5.26), with In(1)w^m4

(Newman et al., 2002)

viable (with Sirt1^5.26)

(Newman et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Sirt1^4.5/Sirt1^2A-7-11 adult males show significantly decreased survival under starvation conditions at 3 weeks (but not 2 weeks) old, compared to controls (relative feeding rate is similar to controls at 1 or 2 weeks old). Sirt1^4.5/Sirt1^2A-7-11 males fed ad libitum show significantly higher levels of glucose and glycogen (at 1 and 2 weeks old) and triglycerides (at 2 but not 1 weeks old) compared to wild type. In fasting conditions, mutants show significantly higher glucose levels (at 2 but not 1 week old), compared to wild type. Sirt1^4.5/Sirt1^2A-7-11 mutants are insulin resistant by 2 weeks of age.

(Palu and Thummel, 2016)

The median lifespan of Sir2^4.5 flies (mutant line generated by outcrossing the mutation into the Canton S background) responds in the same way as that of Canton S controls to a range of diets (from dietary restriction to fully-fed conditions).

(Burnett et al., 2011)

Sir2^4.5/Sir2^5.26 males and females have normal lifespans.

(Balan et al., 2008)

Unlike wild-type flies, the lifespan of Sirt1^4.5/Sirt1^5.26 flies is not extended by a diet of low-calorie food.

(Rogina and Helfand, 2004)

Sir2^4.5/Sir2^5.26 flies are phenotypically normal. Under nonstress environmental conditions, the average median life span of Sir2^4.5/Sir2^5.26 males is slightly, but significantly less than that of heterozygous controls. The average life span is not significantly different from controls. Under stress conditions, the mean life span of Sir2^4.5/Sir2^5.26 males is not significantly different from controls. The median life span of Sir2^4.5/Sir2^5.26 males is significantly longer than that of controls.

(Newman et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Sirt1^4.5/Sirt1^5.26 is a suppressor of long lived phenotype of Naam^UAS.Tag:V5, Scer\GAL4^Tub.PU

(Balan et al., 2008)

NOT Suppressor of

Statement

Reference

Sirt1^4.5/Sirt1^5.26 is a non-suppressor of abnormal neuroanatomy | conditional phenotype of Mmus\Wld^S.UAS, Scer\GAL4^Or22a.8197

(Avery et al., 2009)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Sirt1^4.5/Sirt1^5.26 is a non-suppressor of olfactory receptor neuron | conditional phenotype of Mmus\Wld^S.UAS, Scer\GAL4^Or22a.8197

(Avery et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Two copies of Hnf4^{T:Avic\GFP-SF,T:Zzzz\FLAG} restores normal insulin signaling responses but does not rescue hyperglycemia and elevated glycogen levels in Sirt1^4.5/Sirt1^2A-7-11 flies.

(Palu and Thummel, 2016)

The increase in lifespan caused by expression of Naam^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^elav.PLu is completely suppressed by Sir2^4.5/Sir2^5.26.

(Balan et al., 2008)

Xenogenetic Interactions

Statement

Reference

Sir2^4.5/Sir2^5.26 does not suppress the protective effect on axotomised olfactory receptor neurons in flies expressing Mmus\wld^S.Scer\UAS under the control of Scer\GAL4^Or22a.8197. No change is seen in the amount of Wallerian degeneration 15 days after injury.

(Avery et al., 2009)

Complementation and Rescue Data

Rescued by

Sirt1^4.5/Sirt1^2A-7-11 is rescued by Sirt1^UAS.cPa/Scer\GAL4^r4

(Palu and Thummel, 2016)

Not rescued by

Sirt1^4.5/Sirt1^2A-7-11 is not rescued by Sirt1^UAS.cPa/Scer\GAL4^Mef2.PR

(Palu and Thummel, 2016)

Sirt1^4.5/Sirt1^2A-7-11 is not rescued by Sirt1^UAS.cPa/Scer\GAL4^Ilp2.215-1

(Palu and Thummel, 2016)

Comments

Expression of Sirt1^Scer\UAS.cPa driven in the fat body by Scer\GAL4^r4 (but not when driven by Scer\GAL4^Mef2.PR or Scer\GAL4^Ilp2.215-1) rescues insulin signaling in peripheral tissues of Sirt1^4.5/Sirt1^2A-7-11 flies.

(Palu and Thummel, 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32568

Sirt1^4.5 cn¹/SM6b, P{eve-lacZ8.0}SB1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Sir2^4.5

(Qu et al., 2025, Avery et al., 2009, Balan et al., 2008, Wood et al., 2004)

Sirt1^4.5

dSir2^4.5

(Burnett et al., 2011, Bianchi-Frias et al., 2004, Rogina and Helfand, 2004, Newman et al., 2002)

l(2)K14513^4.5

(Newman et al., 2002)

sir2^4.5

(Palu and Thummel, 2016, Avery et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (9)

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