FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sirt12A-7-11
Open Close
General Information
Symbol
Dmel\Sirt12A-7-11
Species
D. melanogaster
Name
FlyBase ID
FBal0181537
Feature type
allele
Associated gene
Dmel\Sirt1
Associated Insertion(s)
Carried in Construct
Also Known As
sir22A-7-11
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kong et al., 2010)
Mutagen
recombination
(Xie and Golic, 2005.6.1, Furuyama et al., 2004)
I-CreI
(Xie and Golic, 2005.6.1)
ends-in gene targeting
(Xie and Golic, 2005.6.1)
Progenitor genotype
Cytology
Description

Deletion of the Sir2 coding region.

(Xie and Golic, 2005.6.1)

Deletion generated by targeted knockout, which removes the coding sequence precisely.

(Furuyama et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  alcohol use disorder
      CEA
      (Larnerd et al., 2023, Kong et al., 2010)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Sirt12A-7-11/Sirt12A-7-11 mutant flies are less sensitive to the sedating effects of acute ethanol exposure, and also show a marked decrease in sedation tolerance, but do not exhibit alterations in ethanol absorption or metabolism, as compared to controls. Wild type flies pre-exposed to ethanol vapor develop rapid ethanol preference given a choice of liquid food with and without added ethanol, but this preference fails to develop in Sirt12A-7-11/Sirt12A-7-11 mutants, in fact, these mutants are more likely to prefer ethanol-containing food when they have no pre-exposure to ethanol. Sirt12A-7-11/Sirt12A-7-11 mutants do not show a significant difference in aversion to quinine as compared to controls. Sirt12A-7-11/Sirt12A-7-11 flies show strongly reduced conditioned odor preference associated with ethanol intoxication, but no defects in olfactory acuity.

      (Engel et al., 2016)

      Sirt12A-7-11 homozygotes as well as Sirt12A-7-11/Df(2L)ED784 mutant adults have extra scutellar bristles.

      Sirt12A-7-11/Df(2L)ED784 adults display ectopic wing vein material.

      (Horvath et al., 2016)

      Sirt14.5/Sirt12A-7-11 adult males show significantly decreased survival under starvation conditions at 3 weeks (but not 2 weeks) old, compared to controls (relative feeding rate is similar to controls at 1 or 2 weeks old). Sirt14.5/Sirt12A-7-11 males fed ad libitum show significantly higher levels of glucose and glycogen (at 1 and 2 weeks old) and triglycerides (at 2 but not 1 weeks old) compared to wild type. In fasting conditions, mutants show significantly higher glucose levels (at 2 but not 1 week old), compared to wild type. Sirt14.5/Sirt12A-7-11 mutants are insulin resistant by 2 weeks of age.

      (Palu and Thummel, 2016)

      Sirt12A-7-11 mutant adults show reduced survival upon starvation compared to wild-type controls.

      (Banerjee et al., 2012)

      Adult brains have a significant decrease in dopaminergic neuron number (especially in the DL1 cluster) in 30 day old Sirt12A-7-11/Sirt12A-7-11 flies. Climbing ability in Sirt12A-7-11/Sirt12A-7-11 is significantly worse and there is a significant reduction in ATP of the indirect flight muscle compared to wild type at 15 (but not 3) days old.

      (Koh et al., 2012)

      Homozygotes show no obvious morphological or behavioural defects.

      At 60% ethanol vapor dose, Sir22A-7-11 flies show reduced sedation sensitivity, reduced sedation tolerance, and almost no increase in locomotor activity upon repeated ethanol exposure compared to controls. At 73% ethanol vapor dose, Sir22A-7-11 flies retain the latter phenotype, but exhibit normal sedation sensitivity and sedation tolerance.

      Sir22A-7-11/Sir2NP1145 flies show almost no increase in locomotor activity upon repeated ethanol exposure compared to controls.

      (Kong et al., 2010)

      Sir22A-7-11 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32oC or 38oC.

      (Wei and Rong, 2007)

      Heterozygous males do not have ectopic sex combs on the T2 or T3 legs.

      (Furuyama et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Statement
      Reference

      Sirt12A-7-11 has visible | adult stage phenotype, enhanceable by N[+]/N55e11

      (Horvath et al., 2016)

      Sirt12A-7-11 has visible | adult stage phenotype, enhanceable by DeltaRevF10/Dl[+]

      (Horvath et al., 2016)
      NOT Enhanced by
      Statement
      Reference

      Sirt12A-7-11 has decreased cell number | adult stage phenotype, non-enhanceable by Pink1B9

      (Koh et al., 2012)
      Enhancer of
      Statement
      Reference

      Sirt12A-7-11/Sirt12A-7-11 is an enhancer of visible | adult stage phenotype of DeltaRevF10

      (Horvath et al., 2016)

      Sirt12A-7-11/Sirt12A-7-11 is an enhancer of visible | adult stage phenotype of N55e11

      (Horvath et al., 2016)

      Sirt1[+]/Sirt12A-7-11 is an enhancer of visible | adult stage phenotype of N55e11

      (Horvath et al., 2016)

      Sirt1[+]/Sirt12A-7-11 is an enhancer of visible | adult stage phenotype of H2

      (Horvath et al., 2016)

      Sirt12A-7-11/Sirt12A-7-11 is an enhancer of visible | adult stage phenotype of H2

      (Horvath et al., 2016)

      Sir2[+]/Sirt12A-7-11 is an enhancer of visible | dominant | homeotic phenotype of Pcl11

      (Furuyama et al., 2004)

      Sir2[+]/Sirt12A-7-11 is an enhancer of visible | dominant | homeotic phenotype of Pc3

      (Furuyama et al., 2004)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Sirt12A-7-11 has scutellar bristle | increased number phenotype, enhanceable by N[+]/N55e11

      (Horvath et al., 2016)

      Sirt12A-7-11 has scutellar bristle | increased number phenotype, enhanceable by DeltaRevF10/Dl[+]

      (Horvath et al., 2016)
      NOT Enhanced by
      Statement
      Reference

      Sirt12A-7-11 has dorso-lateral dopaminergic neuron phenotype, non-enhanceable by Pink1B9

      (Koh et al., 2012)
      Enhancer of
      Statement
      Reference

      Sirt12A-7-11/Sirt12A-7-11 is an enhancer of scutellar bristle | increased number phenotype of DeltaRevF10

      (Horvath et al., 2016)

      Sirt12A-7-11/Sirt12A-7-11 is an enhancer of scutellar bristle | increased number phenotype of N55e11

      (Horvath et al., 2016)

      Sirt12A-7-11/Sirt12A-7-11 is an enhancer of wing vein phenotype of N55e11

      (Horvath et al., 2016)

      Sirt1[+]/Sirt12A-7-11 is an enhancer of wing vein phenotype of N55e11

      (Horvath et al., 2016)

      Sirt1[+]/Sirt12A-7-11 is an enhancer of wing vein L5 phenotype of H2

      (Horvath et al., 2016)

      Sirt12A-7-11/Sirt12A-7-11 is an enhancer of wing vein L5 phenotype of H2

      (Horvath et al., 2016)

      Sir2[+]/Sirt12A-7-11 is an enhancer of mesothoracic leg phenotype of Pcl11

      (Furuyama et al., 2004)

      Sir2[+]/Sirt12A-7-11 is an enhancer of sex comb | ectopic phenotype of Pcl11

      (Furuyama et al., 2004)

      Sir2[+]/Sirt12A-7-11 is an enhancer of mesothoracic leg phenotype of Pc3

      (Furuyama et al., 2004)

      Sir2[+]/Sirt12A-7-11 is an enhancer of sex comb | ectopic phenotype of Pc3

      (Furuyama et al., 2004)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The formation of extra scutellar bristles observed in each of the following single mutants: Sirt12A-7-11/Sirt12A-7-11, DlRevF10/+ and N55e11/+ is strongly enhanced in either Sirt12A-7-11/Sirt12A-7-11;DlRevF10/+ or N55e11/+;Sirt12A-7-11/Sirt12A-7-11 double mutants, which on average have around seven scutellar bristles (instead of the four normally found in wild-type flies).

      Similarly, the severity (width) of the vein deltas phenotype characteristic for N55e11 heterozygotes is increased by hetero- or (more strongly) homozygosity of Sirt12A-7-11.

      The shortening of the wing vein L5 observed H2 heterozygous adults is partially rescued by combination with one or (more strongly) two copies of Sirt12A-7-11.

      (Horvath et al., 2016)

      Two copies of Hnf4T:Avic\GFP-SF,T:Zzzz\FLAG restores normal insulin signaling responses but does not rescue hyperglycemia and elevated glycogen levels in Sirt14.5/Sirt12A-7-11 flies.

      (Palu and Thummel, 2016)

      Pink1B9/Y does not enhance dopaminergic neuron loss (in the DL1 cluster) in Sirt12A-7-11/Sirt12A-7-11 flies.

      (Koh et al., 2012)

      The frequency of ectopic sex combs seen in Pc3/+ males is increased by Sir22A-7-11; in Sir22A-7-11/+ ; Pc3/+ double heterozygous males, 98% of T2 legs and 61% of T3 legs have sex combs, while in Sir22A-7-11/Sir22A-7-11 ; Pc3/+ males, 99% of T2 legs and 78% of T3 legs have sex combs. The frequency of ectopic sex combs seen in Pcl11/+ males is increased by Sir22A-7-11; in Sir22A-7-11/+ ; Pcl11/+ double heterozygous males, 76% of T2 legs and 35% of T3 legs have sex combs. Eye colour: Sir22A-7-11/+ moderately, though consistently, impairs the silencing of the heterozygous wUbxPRE.2.2 transgene, resulting in a slight increase in eye pigmentation. Eye colour: flies homozygous for wUbxPRE.2.2 show a dramatic increase in eye pigmentation if they are also homozygous for Sir22A-7-11.

      (Furuyama et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Sirt14.5/Sirt12A-7-11 is rescued by Sirt1UAS.cPa/Scer\GAL4r4

      (Palu and Thummel, 2016)
      Partially rescued by

      Sirt12A-7-11 is partially rescued by Scer\GAL4elav-C155/Sirt1UAS.DsRed(Unk)

      (Engel et al., 2016)
      Not rescued by

      Sirt14.5/Sirt12A-7-11 is not rescued by Sirt1UAS.cPa/Scer\GAL4Mef2.PR

      (Palu and Thummel, 2016)

      Sirt14.5/Sirt12A-7-11 is not rescued by Sirt1UAS.cPa/Scer\GAL4Ilp2.215-1

      (Palu and Thummel, 2016)
      Comments

      Expression of Sirt1Scer\UAS.T:Disc\RFP under the control of Scer\GAL4elav-C155 fails to rescue the reduced ethanol sensitivity, but does rescue the reduced tolerance to repeated ethanol exposure seen in Sirt12A-7-11/Sirt12A-7-11 flies.

      (Engel et al., 2016)

      Expression of Sirt1Scer\UAS.cPa driven in the fat body by Scer\GAL4r4 (but not when driven by Scer\GAL4Mef2.PR or Scer\GAL4Ilp2.215-1) rescues insulin signaling in peripheral tissues of Sirt14.5/Sirt12A-7-11 flies.

      (Palu and Thummel, 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      H. Xie K. Golic

      (Furuyama et al., 2004)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (9)
      References (17)