The following information accompanied stocks donated to the Bloomington Stock Center by Heng Xie and Kent Golic, University of Utah.
Sir22A-7-11 is a deletion of the Sir2 coding region produced by ends-in gene targeting followed by I-CreI-induced deletion. PCR primers sir2-C1 (5'-CCAAATGGGTGCGAAGCTGACG-3') and sir2-C2 (5'-GGCCCTCGGCTACGATTTCGCAG-3') give a 0.3kb band for the mutant allele and a 3kb band for the wild type allele. The donor template and technique used to generate this line is described in Xie and Golic, 2004, Genetics 168(3): 1477--1489 (FBrf0180287).
Sirt25B-2-35 is a deletion of Sirt2 coding region produced by ends-in gene targeting followed by I-CreI-induced deletion. PCR primers CG5085-C1 (5'-GCCCCAGGCTAGTCTAAATAG-3') and CG5085-C2 (5'-GAAAGAAAGCTCGCGCTATTAG-3') give a 0.2kb band for the mutant allele and a 1.4kb band for the wild type allele. The donor template and technique used to generate this line is described in Xie and Golic, 2004, Genetics 168(3): 1477--1489 (FBrf0180287).
Sirt4white+1 is a deletion of Sirt4 coding region produced by ends-out targeting. The miniwhite marker was not removed by Cre-mediated recombination.