Open Close
General Information
Symbol
Mmus\WldS.UAS
Species
M. musculus
Name
FlyBase ID
FBal0258015
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-WLDS, UAS-Wlds, 5xUAS-wldS
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the 'Wld[s]' mutant form of Mmus\Wld.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Class IV dendritic arborization neurons expressing Mmus\WldS.UAS under the control of Scer\GAL4DcG fail to fragment and clear dendrite following dendrite injury, as compared to controls.

Expression of 5 copies of Mmus\wldS.Scer\UAS under the control of Scer\GAL4VGlut-OK371 has a strong protective effect on L1 vein neurons following axotomy. Whereas wild type axons undergo fragmentation, many severed but intact axons are observed.

Expression of Mmus\wldS.Scer\UAS under the control of Scer\GAL4Or22a.7.717 suppresses Wallerian degeneration of axotomized olfactory receptor neuron axons.

Expression of Mmus\wldS.Scer\UAS in olfactory receptor neurons under the control of Scer\GAL4peb-GAL4 blocks signaling from severed axons resulting in severed axons not being cleared from the nervous system and remaining intact after several days. No plasticity is induced in Mmus\wldS.Scer\UAS-expressing flies.

Expression of Mmus\wldS.Scer\UAS in ddaC neurons under the control of Scer\GAL4ppk.1.9 can dramatically delay dendrite degeneration after injury. Dendrites are still present 18 hours after severing, compared to the complete clearance of dendritic debris in controls.

Pruning of ddaC dendrites is delayed upon expression of Mmus\wldS.Scer\UAS under the control of Scer\GAL4ppk.1.9.

Expression of Mmus\wldS.Scer\UAS in the ddaC neurons (using the Scer\GAL4ppk.PG line) delays removal of ddaC dendritic branches at 18 hours after puparium formation.

Expression of Mmus\wldS.Scer\UAS under the control of Scer\GAL4Or22a.8197 has a protective effect on olfactory receptor neurons following axotomy: 77% of olfactory receptor neuron axons remain intact 30 days after injury.

Expression of Mmus\wldS.Scer\UAS in antennal ORNs using Scer\GAL4Or22a.8197 can protect severed axons from Wallerian degeneration for weeks, with Mmus\wldS.Scer\UAS expressing exons exhibiting largely normal morphology. By 50 days after injury, the axons of Mmus\wldS.Scer\UAS-expressing neurons have degenerated significantly, with approximately 20% of antennal lobes containing detectable axon fibers. All severed axons ultimately degenerate between 30 and 50 days after injury.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The loss of multidendritic ddaC neurons in third instar larvae induced by Ect4ΔARM.Scer\UAS.T:Hsap\MYC expression (with the Scer\GAL4ppk.PG driver) in third instar larvae as well as neurite degeneration in Pdf-positive neurons in adult flies (under the Scer\GAL4P2.4.Pdf driver combined with tub-Gal80[ts] to restrict the expression to adulthood) can be partially improved by co-expression of Mmus\WldS.Scer\UAS.

The loss of olfactory receptor neuron (ORN) cell bodies and axons and the morphological defects in the antennal lobe neuropil which are seen when homozygous milt33-853 ORN clones are induced is not suppressed by expression of Mmus\wldS.Scer\UAS under the control of Scer\GAL4Or22a.7.717.

Expression of Mmus\wldS.Scer\UAS under the control of Scer\GAL4elav-C155 protects both wild-type and milt33-853 motor neuron clones from degeneration after axotomy at 16 hours after crushing and delays Wallerian degeneration in both wild-type and milt33-853 motor neuron clones 24 hours after crushing.

Co-expression of Mmus\wldS.Scer\UAS and BacA\p35Scer\UAS.cHa in ddaC neurons (under the control of Scer\GAL4ppk.PG) results in significantly better protection of dendrites than flies expressing either transgene alone.

Co-expression of TER94GD9777 suppresses the protective effect on axotomised olfactory receptor neurons in flies expressing Mmus\wldS.Scer\UAS under the control of Scer\GAL4Or22a.8197. An increased amount of Wallerian degeneration is seen 15 days after injury. No effect is seen in uninjured age-matched controls.

Co-expression of Sir2NIG.5216R does not suppress the protective effect on axotomised olfactory receptor neurons in flies expressing Mmus\wldS.Scer\UAS under the control of Scer\GAL4Or22a.8197. No change is seen in the amount of Wallerian degeneration 15 days after injury.

Sir24.5/Sir25.26 does not suppress the protective effect on axotomised olfactory receptor neurons in flies expressing Mmus\wldS.Scer\UAS under the control of Scer\GAL4Or22a.8197. No change is seen in the amount of Wallerian degeneration 15 days after injury.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Mmus\WldS.Scer\UAS
Mmus\WldS.UAS
Mmus\wldS.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (17)