FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
hry, Sirt1
FlyBase ID
FBig0000097751
Interaction Network
Interactions Browser links
hry network
Sirt1 network
Reported Interactions
FBrf0148924-1.PD.A
Reference
Rosenberg and Parkhurst, 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hry 

Hairy

bait

GST tag

hry 
hry 
Sirt1 

Sir2

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hry 
basic domain
sufficient binding region
aa 29-48
hry 
basic domain
necessary binding region
aa 43-46

RRAR motif

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Rosenberg and Parkhurst, 2002)

Sir2 binds to h and dpn, but not to E(spl)m3-HLH or E(spl)m8-HLH.

(Rosenberg and Parkhurst, 2002)
FBrf0148924-3.PD.WB
Reference
Rosenberg and Parkhurst, 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
hry 

Hairy

bait

GST tag

hry 
Sirt1 

Sir2

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
hry 
bHLH domain
sufficient binding region
aa 29-90
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Rosenberg and Parkhurst, 2002)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)