FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Sirt1, nej
FlyBase ID
FBig0000116370
Interaction Network
Interactions Browser links
Sirt1 network
nej network
Reported Interactions
FBrf0215291-1.coIP.WB
Reference
Smolik, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sirt1 

dSIR2

bait
nej 

dCBP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Smolik, 2009)
FBrf0215291-2.PD.A
Reference
Smolik, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nej 

dCBP

bait

GST tag

Sirt1 

dSIR2

prey

[35]S label

Sirt1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sirt1 
interaction domain
sufficient binding region
aa 461-821
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Smolik, 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)