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Bauer, J.H., Morris, S.N., Chang, C., Flatt, T., Wood, J.G., Helfand, S.L. (2009). dSir2 and Dmp53 interact to mediate aspects of CR-dependent lifespan extension in D. melanogaster.  Aging 1(1): 38--48.
FlyBase ID
FBrf0215184
Publication Type
Research paper
Abstract

Calorie Restriction (CR) is a well established method of extending life span in a variety of organisms. In the fruit fly D. melanogaster, CR is mediated at least in part by activation of dSir2. In mammalian systems, one of the critical targets of Sir2 is the tumor suppressor p53. This deacetylation of p53 by Sir2 leads to inhibition of p53's transcriptional activity. We have recently shown that inhibition of Dmp53 activity in the fly brain through the use of dominant-negative (DN) constructs that inhibit DNA-binding can extend life span. This life span extension appears to be related to CR, as CR and DN-Dmp53 donot display additive effects on life span. Here we report that life span extension by DN-Dmp53 expression is highly dynamic and can be achieved even when DN-Dmp53 is expressed later in life. In addition, we demonstrate that life span extension by activation of dSir2 and DN-Dmp53 expression are not additive. Furthermore, we show that dSir2 physically interacts with Dmp53 and can deacetylate Dmp53-derived peptides. Taken together, our data demonstrate that Dmp53 is a down stream target of dSir2 enzymatic activity and mediates some aspects of the life span extending effects of CR.

PubMed ID
PubMed Central ID
PMC2765060 (PMC) (EuropePMC)
DOI
Related Publication(s)
Note

Longevity regulation in flies: a role for p53.
Donehower, 2009, Aging 1(1): 6--8 [FBrf0215005]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Aging
    Title
    Aging
    ISBN/ISSN
    1945-4589
    Data From Reference
    Genes (2)
    Physical Interactions (1)