dorsal appendage (with bwkCT)
Stages 10B and early 11 of dorsal appendage morphogenesis proceed normally in bwk151/bwkΔ11 egg chambers. Defects in both roof and floor formation become evident in the developing dorsal appendage shortly after morphogenesis begins. Although apical constriction occurs normally in roof cells, the roof cell apices fail to reorganise from an almond shape into a normal triangular array by late stage 11. Instead, the roof cells form a blunt array, that even in stage 12, is abnormally short and/or wide. Floor cells begin to elongate normally, extending beneath the nascent roof in late stage 11, but they show defects in several subsequent stages; they frequently separate along their basolateral margins during tube formation, extend far too laterally and have apical gaps that result in an incomplete tube floor. Even when the roof population attains a normal length, the floor cells of the dorsal appendage primordium often extend too far laterally and have basal discontinuities. During anterior extension, the floor cell population becomes increasingly disorganised, often forking into large clefts. The roof cell population can also bifurcate. This bifurcation correlates with the secretion of prongs and spurs of chorion that project off the main dorsal appendage in the mutant. Defects are also seen in paddle formation; the mutant cells remain 27% more elongated during paddle formation than wild-type cells. Stages 10B and early 11 of dorsal appendage morphogenesis proceed normally in bwk151/bwk08482 egg chambers in culture. However, defects are seen in later stages. Anterior extension of the developing dorsal appendage often occurs abnormally; although it may begin normally, the dorsal appendage forming cells do not advance to the anterior of the egg chamber, the two dorsal appendages are often asymmetrical and the lumens are often either bent or expanded. Cultured egg chambers also show other defects including rounded-up and delaminating follicle cells. bwk151/bwk08482 dorsal appendages have an abnormally wide "moose-antler" shape.
bwkCT/bwk151 has dorsal appendage phenotype, enhanceable by Src42A[+]/Src42AE
bwk151, cic08482 has dorsal appendage phenotype, non-enhanceable by bskUAS.cBa/Scer\GAL4c415
bwk151, cic08482 has dorsal appendage phenotype, non-enhanceable by Scer\GAL4CY2/SharkUAS.cFa
bwk151, cic08482 has dorsal appendage phenotype, non-enhanceable by sax[+]/sax5
bwk151, cic08482 has dorsal appendage phenotype, non-enhanceable by dpp10638/dpp[+]
bwk151, cic08482 has dorsal appendage phenotype, non-enhanceable by bsk2/bsk[+]
bwk151, cic08482 has dorsal appendage phenotype, non-enhanceable by tkv7/tkv[+]
bwk151, cic08482 has dorsal appendage phenotype, suppressible | partially by Scer\GAL4c415/SharkUAS.cFa
bwk151, cic08482 has dorsal appendage phenotype, suppressible | partially by Scer\GAL455B/SharkUAS.cFa
bwk151, cic08482 has dorsal appendage phenotype, suppressible by Scer\GAL4c415/Src42ACA.UAS
bwk151, cic08482 has dorsal appendage phenotype, non-suppressible by bskUAS.cBa/Scer\GAL4c415
bwk151, cic08482 has dorsal appendage phenotype, non-suppressible by Scer\GAL4CY2/SharkUAS.cFa
bwk151, cic08482 has dorsal appendage phenotype, non-suppressible by sax[+]/sax5
bwk151, cic08482 has dorsal appendage phenotype, non-suppressible by dpp10638/dpp[+]
bwk151, cic08482 has dorsal appendage phenotype, non-suppressible by bsk2/bsk[+]
bwk151, cic08482 has dorsal appendage phenotype, non-suppressible by tkv7/tkv[+]
The mutant dorsal appendage phenotype seen in bwk151/bwk08482 eggs is partially suppressed by the expression of sharkScer\UAS.cFa under the control of Scer\GAL4c415 or Scer\GAL455B. Expression of sharkScer\UAS.cFa under the control of Scer\GAL4CY2 has little effect on the mutant dorsal appendage phenotype seen in bwk151/bwk08482 eggs. The mutant dorsal appendage phenotype seen in bwk151/bwk08482 eggs is not significantly modified by bsk2/+, dpp10638/+, sax5/+ or tkv7/+. The mutant dorsal appendage phenotype seen in bwk151/bwk08482 eggs is not significantly modified by expression of bskScer\UAS.cBa under the control of Scer\GAL4c415. The mutant dorsal appendage phenotype seen in bwk151/bwk08482 eggs is suppressed by expression of Src42ACA.Scer\UAS under the control of Scer\GAL4c415. The mutant dorsal appendage phenotype seen in bwk151/bwkCT eggs is enhanced by Src42AE/+.
R. Kelly.