Open Close
General Information
Symbol
Dmel\sax5
Species
D. melanogaster
Name
FlyBase ID
FBal0050459
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C7808306A

Reported nucleotide change:

C?A

Amino acid change:

A289D | sax-PA; A254D | sax-PB; A301D | sax-PC

Reported amino acid change:

A289D

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A missense mutation altering a highly conserved Ala residue affecting a region responsible for ATP binding in the cytoplasmic kinase domain.

Amino acid replacement: A289D.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The neuromuscular junctions of sax4/sax5 homozygous transheterozygous third instar larvae present significantly less boutons than controls.

sax5 heterozygous females show the expected mating-induced increase in germline stem cells in the germarium.

sax4/sax5 third instar larval C4da neurons do not show any obvious defects in the dendritic arborization, as compared to controls.

sax5/Df(2R)H23 mutant third instar larvae show increased uptake and transport of ingested fluorescently labeled fatty acids from the midgut lumen to the fat body compared to wild-type controls.

Large posterior clones of sax5 show significant loss of longitudinal vein 4 (L4) and a narrowing of the L4/L5 intervein.

Less than 5% of sax5/Df(2R)H23 hemizygous mutant larvae exhibit developmental delay, lethargy, a reduction in the size of imaginal discs, brain | larval stage, and midgut structures, as well as trachea | larval stagel truncations.

The evoked excitatory junctional potential (EJP) (measured at muscle 6 of segment A3) shows a decrease in amplitude in sax4/sax5 animals compared to wild type. Quantal content is reduced compared to wild type.

Females deficient for ovarian sax function produce two classes of abnormal eggs. The majority class has abnormal dorsal appendages, the minority class shows reduced egg length as well as abnormal dorsal appendages. In germline clones, only 10-25% or normal number of eggs is produced, over a much briefer time period than for wild type. Most are normal though 20% show aberrant dorsal appendages and short length. Clones display degenerating mid-stage chambers, and produce anterior-open eggs.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
NOT Enhancer of
Statement
Reference

sax[+]/sax5 is a non-enhancer of dorsal appendage phenotype of bwk08482/bwk151

NOT Suppressor of
Statement
Reference

sax5 is a non-suppressor of wing phenotype of dppd5/dpphr56

sax[+]/sax5 is a non-suppressor of dorsal appendage phenotype of bwk08482/bwk151

Other
Statement
Reference

NPFRsk8, sax[+]/sax5 has female germline stem cell | conditional phenotype

Additional Comments
Genetic Interactions
Statement
Reference

NPFRsk8, sax5 double heterozygous females fail to show the expected mating-induced increase in germline stem cells in the germarium, as compared to heterozygous controls.

When dpp[hr4]/+ males are crossed to sax5/+ females, the resulting dpphr4/+ progeny do not show any significant drop in viability compared to the dpphr4/+ progeny of wild-type mothers in control crosses.

sax5 does not suppress the dppd5/dpphr56 wing phenotype. sax5 enhances the lethality associated with dppd5/dpphr56.

The mutant dorsal appendage phenotype seen in bwk151/bwk08482 eggs is not significantly modified by sax5/+.

No interaction with tkv6, E(tkv)D1D1, MadD3, MadD15, MadD24, gbbD4, gbbD8 or gbbD20 is seen in double heterozygous flies. Double heterozygotes with tkvD17, E(tkv)D2D2, MadD14 or MadD16 may show imaginal disc development defects.

Dominantly reduces the viability of gbb1/gbb4 flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Females lacking ovarian function of sax can be produced by rescuing the zygotic lethality of sax5/Df(2R)sax-H9 alleles with saxhs.PB then maintaining the females at non-heat shock temperatures.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (14)