The neuromuscular junctions of sax4/sax5 homozygous transheterozygous third instar larvae present significantly less boutons than controls.
sax5 heterozygous females show the expected mating-induced increase in germline stem cells in the germarium.
sax4/sax5 third instar larval C4da neurons do not show any obvious defects in the dendritic arborization, as compared to controls.
sax5/Df(2R)H23 mutant third instar larvae show increased uptake and transport of ingested fluorescently labeled fatty acids from the midgut lumen to the fat body compared to wild-type controls.
Large posterior clones of sax5 show significant loss of longitudinal vein 4 (L4) and a narrowing of the L4/L5 intervein.
Less than 5% of sax5/Df(2R)H23 hemizygous mutant larvae exhibit developmental delay, lethargy, a reduction in the size of imaginal discs, brain | larval stage, and midgut structures, as well as trachea | larval stagel truncations.
The evoked excitatory junctional potential (EJP) (measured at muscle 6 of segment A3) shows a decrease in amplitude in sax4/sax5 animals compared to wild type. Quantal content is reduced compared to wild type.
Females deficient for ovarian sax function produce two classes of abnormal eggs. The majority class has abnormal dorsal appendages, the minority class shows reduced egg length as well as abnormal dorsal appendages. In germline clones, only 10-25% or normal number of eggs is produced, over a much briefer time period than for wild type. Most are normal though 20% show aberrant dorsal appendages and short length. Clones display degenerating mid-stage chambers, and produce anterior-open eggs.