Germaria from one week old females are smaller than wild type and contain only one or two stem cells. Older females may have none, though though cysts and egg chambers remain.
Female adult escapers are less viable than their heterozygous siblings. Rare embryos from these females show a phenotype similar to but milder and more variable than those from sax1/sax2 females. Homozygous embryos are lacking the second midgut constriction. Homozygous females often lack the anterior crossvein, and the eyes are small due to reduced numbers of ommatidia.