Mutation maps within kinase subdomain XI.
Nucleotide substitution: A?T.
Amino acid replacement: K316term.
A10247899T
A?T
K316term | bsk-PB; K316term | bsk-PE; K316term | bsk-PF
K316term
amnioserosa (with bsk1)
axon & dorsal cluster neuron | somatic clone
embryo | embryonic stage 14 (with bsk1)
filamentous actin (with bsk1)
bsk2 mutant clones in sensory neurons in adult wing do not display any defects in injury-induced axon degeneration (following an axotomy, the severed axons are cleared away normally).
bsk2 heterozygosity does not significantly affect the mitotic index in the adult midgut epithelium, as compared to controls.
bsk2 heterozygous mutants exhibit extreme sensitivity to 1-octen-3-ol. These flies survive for approximately 14 days when exposed to 0.5ppm 1-octen-3-ol, significantly lower than the 18 days-long survival span observed for exposed wild-type flies.
Single cell bsk2 clones in mushroom bodies have axons with wild type projections.
A bsk2/+, background suppressed the rough eye phenotype found upon expression of TgA.Scer\UAS under the control of Scer\GAL4GMR.PS.
The cleft notum phenotype caused by expression of Nf-YAdsRNA.231-399.Scer\UAS.WIZ under the control of Scer\GAL4pnr-MD237 is enhanced by bsk2/+.
Single cell bsk2 dorsal cluster neuron clones, created using the MARCM technique, show no axonal outgrowth.
A greater number of bsk2/+ mutant flies die after feeding with paraquat than wild-type flies.
Homozygous embryos have a dorsal open phenotype. The dorsal hole extends posterior to 50% egg length in 24% of embryos.
Initial phases of dorsal closure are normal, but the first rows under the leading cell row of the dorsal edge show only a partial lengthening cell shape change or no cell shape change at all. The defect is more pronounced in anterior cells.
Mutant embryos show a dorsal hole due to a defect in dorsal closure. Dorsoventral patterning is not defective.
bsk2 has chemical sensitive | dominant phenotype, suppressible by pucE69/puc[+]
bsk2/bsk[+] is an enhancer of lethal | recessive phenotype of Src42AJp45
bsk2/bsk[+] is a non-enhancer of visible phenotype of Scer\GAL4en-e16E, kermitGS2053
bsk2/bsk[+] is a non-enhancer of abnormal planar polarity phenotype of MtlUAS.cMa, Scer\GAL4hs.2sev
bsk2/bsk[+] is a suppressor of abnormal neuroanatomy | larval stage phenotype of Eaat1hypo
bsk2/bsk[+] is a suppressor of increased occurrence of cell division | adult stage phenotype of Scer\GAL4NP5130, UvragHMS01357
bsk2/bsk[+] is a suppressor of abnormal cell migration phenotype of CskGD9345, Scer\GAL4ptc-559.1
bsk2/bsk[+] is a suppressor of abnormal cell migration phenotype of Scer\GAL4ptc-559.1, Sin3AKK100700
bsk2/bsk[+] is a suppressor | partially of visible phenotype of DrefUAS.cSa, Scer\GAL4GMR.PS
bsk2/bsk[+] is a suppressor | partially of visible phenotype of Rab11N124I.UAS, Scer\GAL4GMR.PF
bsk2 is a suppressor | partially of increased cell death phenotype of Rab11RNAi.UAS.WIZ, Scer\GAL4GMR.PF
bsk2/bsk[+] is a suppressor | partially of visible phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca-537.4
bsk2 is a suppressor of visible phenotype of RetMEN2B.GMR
bsk2 is a suppressor of visible phenotype of RetMEN2A.GMR
bsk2/bsk[+] is a suppressor of chemical resistant | dominant phenotype of pucE69
bsk2/bsk[+] is a suppressor of increased cell death phenotype of Scer\GAL4GMR.PF, egrUAS.cMa
bsk2/bsk[+] is a suppressor | partially of abnormal planar polarity phenotype of peb1
bsk2 is a suppressor of visible phenotype of Scer\GAL4GMR.PF, Tak1UAS.cTa
bsk2/bsk[+] is a suppressor of abnormal planar polarity phenotype of Scer\GAL4hs.2sev, msnEP549
bsk2/bsk[+] is a suppressor of abnormal planar polarity phenotype of dshhs.sev.B
bsk2 is a suppressor of abnormal planar polarity phenotype of Scer\GAL4hs.2sev, dshUAS.cNa
bsk2 is a suppressor of abnormal planar polarity phenotype of Scer\GAL4hs.2sev, fzUAS.cAa
bsk2 is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of SarmΔARM.UAS.Tag:MYC, Scer\GAL4nSyb.PS
bsk2/bsk[+] is a non-suppressor of visible phenotype of Scer\GAL4en-e16E, kermitGS2053
bsk2/bsk[+] is a non-suppressor of lethal - all die during embryonic stage phenotype of POSHUAS.cSa, Scer\GAL4pnr-MD237
bsk2/bsk[+] is a non-suppressor of abnormal planar polarity phenotype of MtlUAS.cMa, Scer\GAL4hs.2sev
bsk2/bsk[+] is an enhancer of mesonotum phenotype of Nf-YARNAi.231-399.UAS.WIZ, Scer\GAL4pnr-MD237
bsk2/bsk2 is an enhancer of border follicle cell phenotype of PvrDN.UASp, Scer\GAL4slbo.2.6
bsk2/bsk[+] is an enhancer of wing hair | increased number phenotype of Scer\GAL4ap-md544, trcT453A.UAS.L
bsk2 is an enhancer of mesonotum phenotype of PvrRNAi.UAS, Scer\GAL4pnr-MD237
bsk2 is an enhancer of thorax phenotype of PvrRNAi.UAS, Scer\GAL4pnr-MD237
bsk2/bsk[+] is a non-enhancer of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053
bsk2/bsk[+] is a non-enhancer of ommatidium phenotype of MtlUAS.cMa, Scer\GAL4hs.2sev
bsk2 is a non-enhancer of photoreceptor cell | precursor & nucleus phenotype of Scer\GAL4unspecified, msnDN.UAS
bsk2/bsk[+] is a non-enhancer of dorsal appendage phenotype of bwk151, cic08482
bsk2/bsk[+] is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1hypo
bsk2/bsk[+] is a suppressor of NMJ bouton | increased number | larval stage phenotype of Eaat1hypo
bsk2/bsk[+] is a suppressor of adult midgut epithelium phenotype of Scer\GAL4NP5130, UvragHMS01357
bsk2/bsk[+] is a suppressor of wing disc phenotype of CskGD9345, Scer\GAL4ptc-559.1
bsk2/bsk[+] is a suppressor of wing disc phenotype of Scer\GAL4ptc-559.1, Sin3AKK100700
bsk2/bsk[+] is a suppressor | partially of eye phenotype of DrefUAS.cSa, Scer\GAL4GMR.PS
bsk2/bsk[+] is a suppressor of eye phenotype of Scer\GAL4GMR.PS, TgA.UAS
bsk2/bsk[+] is a suppressor of ommatidium phenotype of Scer\GAL4GMR.PS, TgA.UAS
bsk2/bsk[+] is a suppressor of interommatidial bristle phenotype of Scer\GAL4GMR.PS, TgA.UAS
bsk2/bsk[+] is a suppressor | partially of eye phenotype of Rab11N124I.UAS, Scer\GAL4GMR.PF
bsk2/bsk[+] is a suppressor | partially of ommatidium phenotype of ecspok
bsk2/bsk[+] is a suppressor | partially of microchaeta phenotype of Rala35d
bsk2/bsk[+] is a suppressor of macrochaeta phenotype of Rala35d
bsk2/bsk[+] is a suppressor | partially of microchaeta phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca-537.4
bsk2/bsk[+] is a suppressor of macrochaeta phenotype of RalaS25N.cMa.UAS, Scer\GAL4sca-537.4
bsk2/bsk[+] is a suppressor of eye phenotype of pucA251.1F3
bsk2 is a suppressor of eye phenotype of RetMEN2B.GMR
bsk2 is a suppressor of eye phenotype of RetMEN2A.GMR
bsk2 is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF, egrGS9830
bsk2/bsk[+] is a suppressor of ommatidium phenotype of rprGMR.PW
bsk2/bsk[+] is a suppressor of eye phenotype of Scer\GAL4GMR.PF, egrUAS.cMa
bsk2/bsk[+] is a suppressor | partially of ommatidium phenotype of peb1
bsk2 is a suppressor of ommatidium phenotype of Rac1V12.hs.sev
bsk2 is a suppressor of eye phenotype of Scer\GAL4GMR.PF, Tak1UAS.cTa
bsk2 is a suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, msnEP549
bsk2/bsk[+] is a suppressor of ommatidium phenotype of dshhs.sev.B
bsk2/bsk[+] is a suppressor of scutum & macrochaeta phenotype of RalaS25N.UAS, Scer\GAL4sca-537.4
bsk2 is a suppressor of phenotype of dshhs.sev.B
bsk2 is a suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, dshUAS.cNa
bsk2 is a suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, fzUAS.cAa
bsk2 is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of SarmΔARM.UAS.Tag:MYC, Scer\GAL4nSyb.PS
bsk2 is a non-suppressor of axon | somatic clone | adult stage phenotype of SarmΔARM.UAS.Tag:MYC, Scer\GAL4nSyb.PS
bsk2/bsk[+] is a non-suppressor of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053
bsk2/bsk[+] is a non-suppressor of ommatidium phenotype of MtlUAS.cMa, Scer\GAL4hs.2sev
bsk2/bsk[+] is a non-suppressor of dorsal appendage phenotype of bwk151, cic08482
bsk2 is a non-suppressor of phenotype of dshΔC.hs.sev
bsk2, msn[+]/msn102 has embryonic/first instar larval cuticle | dorsal phenotype
bsk2/bsk[+], msn102 has embryonic/first instar larval cuticle | dorsal phenotype
bsk2, cnomis1/cno3 has embryonic/first instar larval cuticle | dorsal phenotype
bsk2/bsk[+], cnomis1/cno3 has embryonic/first instar larval cuticle | dorsal phenotype
The axon degeneration in sensory neurons clones in the adult wing expressing Ect4ΔARM.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nSyb.PS can be suppressed by combination with bsk2.
bsk2 heterozygosity suppresses the increased mitotic index in the adult midgut epithelium induced by the adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression).
A bsk2 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
The cell invasion phenotype seen when Sin3AKK100700 is strongly expressed (using a line in which the transgene has been mobilised to the third chromosome) under the control of Scer\GAL4ptc-559.1 is suppressed in a bsk2/+ background.
The rough eye phenotype caused by expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS is weakly but significantly suppressed by by bsk2/+.
bsk2/+ is unable to suppress the Scer\GAL4pnr-MD237>POSHScer\UAS.cSa lethality phenotype.
The eye defects seen in Rab11mo homozygotes are partially suppressed by bsk2/+.
The reduced eye size caused by expression of Rab11N124I.Scer\UAS under the control of Scer\GAL4GMR.PF is partially suppressed by bsk2/+.
Homozygous bsk2 border follicle cell clones that are also expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 show defects in migration that are more severe than border follicle cells expressing PvrDN.Scer\UAS under the control of Scer\GAL4slbo.2.6 in an otherwise wild-type background.
bsk2 does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; bsk2 clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, the proportion of Df(1)su(s)R194/+ cells in the larval eye disc is not increased by the presence of bsk2.
A bsk2/+ background enhances the splitting wing hair phenotype of flies that express trcT453A.Scer\UAS under the control of Scer\GAL4ap-md544.
7% of animals expressing msnDN.Scer\UAS in the developing eye, combined with bsk2/+ exhibit a R-cell nuclear migration phenotype.
Heterozygosity for bsk2 partially rescues pucA251.1F3 mutant eye tissue.
Shows no interaction with Mpk21.
The addition of bsk2/+ to egrGS9830, Scer\GAL4GMR.PF animals leads to a suppression of the eye phenotype.
bsk2 suppresses the ommatidial polarity defects seen in dshhs.sev.B flies grown at 29oC. About 85% of ommatidia are in their correct polarity compared to 55% in dshhs.sev.B flies alone. Dominantly suppresses the ommatidial polarity phenotype seen in flies with msnEP549 driven by Scer\GAL4hs.2sev, producing near wild-type ommatidial arrays.
The dorsal hole defect, though not embryonic lethality, can be partly rescued by bskhs.PS.
Cuticle phenotypes reveal an allelic series: bskJ27 < bsk1 < bsk2 < bskflp147E.