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General Information
Symbol
Dmel\DrefUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Sawado
FlyBase ID
FBal0093422
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DREF, UAS-DREF1-709, UAS-dDREF
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Dref is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS results in a rough eye phenotype.

Over-expression of DrefScer\UAS.cSa using either Scer\GAL4sca-109-68 or Scer\GAL4ac.13 does not induce ectopic sensory organ precursors nor macrochaetes in the adult thorax, nor ectopic campaniform sensilla on the L3 wing vein.

Over-expression of DrefScer\UAS.cSa using Scer\GAL4sca-109-68 does not change the nuclear or nucleolar size of the posterior scutellar shaft cells.

Overexpression of DrefScer\UAS.cSa in the developing eye (under the control of Scer\GAL4GMR.PS) induces ectopic DNA synthesis and apoptosis and inhibits photoreceptor cell differentiation in eye imaginal discs, such that adult flies exhibit a mild rough eye phenotype.

Scer\GAL4GMR.PS DrefScer\UAS.cSa flies have a severe rough eye phenotype.

Scer\GAL4GMR.PS-mediated expression of DrefScer\UAS.cSa results in a severe rough eye phenotype, though viability and fertility are normal.

Flies expressing DrefScer\UAS.cSa under the control of Scer\GAL4ptc-559.1 show ectopic macrochaetae in the scutellum and a notching wing phenotype and reduction in the distance between the L3 and L4 wing veins.

Flies expressing DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS have a severe rough eye phenotype.

DrefScer\UAS.cSa-overexpression under the control of Scer\GAL4GMR.PS induces apoptosis in eye imaginal disc cells. Overexpression of Scer\GAL4GMR.PS>DrefScer\UAS.cSa also results in the loss of membrane potential seen in wild-type mitochondria.

Expression of DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS results in a severe rough eye phenotype.

Expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS results in a rough eye phenotype.

Expression of DrefScer\UAS.cSa under the control of Scer\GAL4en-e16E results in an increase in the width of the distal end of wing vein L5.

Expression of DrefScer\UAS.cSa in the eye under the control of Scer\GAL4GMR.PF causes severe eye degeneration, with loss of pigmentation.

Expression of DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS leads to an adult eye which is severely rough in appearance and with most bristles missing. The organised array of each ommatidium is destroyed and some ommatidia are fused to each other. The number and shape of the photoreceptor cells in each ommatidia is also abnormal. Pigment cells are found to be missing in all ommatidia, and some remaining tissues are vacuolated.

No significant difference is seen between control and DrefScer\UAS.cSa-expressing flies (under the control of Scer\GAL4GMR.PS) in the region anterior to the morphogenetic furrow, where the GMR promoter is inactive. In control discs, a 30-min pulse of BrdU incorporation leads to labeling of cells in one or two columns posterior to the morphogenetic furrow, while imaginal discs of transgenic flies expressing DrefScer\UAS.cSa labels the equivalent of five columns. Therefore expression of DrefScer\UAS.cSa expands the S phase or induces an extra S phase. Most of the nuclei of cells with BrdU incorporated occupy a more apical position in the imaginal discs than do nuclei in the synchronous S-phase cells posterior to the morphogenic furrow and in uncommitted cells, suggesting that DrefScer\UAS.cSa expression causes ectopic S phase in some cells that are normally postmitotic.

Expression of DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS inhibits the differentiation of R1, R6 and R7 photoreceptor cells.

Transgenic flies expressing DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS exhibit an increase in apoptotic cells in the region posterior to the morphogenetic furrow. Apoptosis seems to begin in the imaginal disc cells in the region where commitment to R1/R6 cells takes place, suggesting that failure of differentiation in to R1/R6 may induce apoptosis.

Expression of DrefScer\UAS.cSa under the control of Scer\GAL4Act88F.PD or Scer\GAL4ptc-559.1 results in a rough eye phenotype. Expression of DrefScer\UAS.cSa under the control of Scer\GAL41854 results in a weak rough eye phenotype. Expression of DrefScer\UAS.cSa under the control of Scer\GAL4en-e16E results in a notched wing in the posterior compartment. Expression of DrefScer\UAS.cSa under the control of Scer\GAL4ap-md544 results in a crumpled wing. Expression of DrefScer\UAS.cSa under the control of Scer\GAL4dpp.blk1 results in notching of the wing between veins L3 and L4. Expression of DrefScer\UAS.cSa under the control of Scer\GAL4Dll-md23 results in notching of the peripheral wing margin. Severe wing notching is seen in about 5% of flies. Expression of DrefScer\UAS.cSa under the control of Scer\GAL4twi.PG, Scer\GAL4prd.RG1, Scer\GAL44935, Scer\GAL455B, Scer\GAL41882, Scer\GAL4rho.PL, Scer\GAL4wg.PM, Scer\GAL4elav.PLu, Scer\GAL42689, Scer\GAL4nos.PG or Scer\GAL4l(1)BP-1 has no effect on wing morphology. Expression of DrefScer\UAS.cSa under the control of Scer\GAL4en-e16E results in an increase in apoptotic cells in the posterior compartment of the wing disc. Expression of DrefScer\UAS.cSa under the control of Scer\GAL4ap-md544 results in an increase in apoptotic cells in the dorsal region of the wing disc.

Overexpression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS results in a rough eye phenotype.

Expression of DrefCR1.Scer\UAS under the control of Scer\GAL4hs.PB using heat shock causes lethality throughout developmental stages. The lethality is suppressed by coexpression of DrefScer\UAS.cSa.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

DrefUAS.cSa, Scer\GAL4GMR.PS has visible phenotype, suppressible | partially by bsk1/bsk[+]

DrefUAS.cSa, Scer\GAL4GMR.PS has visible phenotype, suppressible | partially by bsk2/bsk[+]

DrefUAS.cSa, Scer\GAL4GMR.PS has visible phenotype, suppressible | partially by eIF-4a[+]/eIF4A02439

DrefUAS.cSa, Scer\GAL4GMR.PS has visible phenotype, suppressible by skpA[+]/SkpA1

DrefUAS.cSa, Scer\GAL4en-e16E has visible phenotype, suppressible by phl[+]/RafE1

NOT suppressed by
Statement
Reference

DrefUAS.cSa, Scer\GAL4en-e16E has visible phenotype, non-suppressible by E2f[+]/E2f191

NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference

DrefUAS.cSa has eye phenotype, enhanceable by Df(1)C128/+

DrefUAS.cSa has eye phenotype, enhanceable by Tom40G0216/Tom40[+]

DrefUAS.cSa has eye phenotype, enhanceable by Ykt6[+]/Ykt6G0155

DrefUAS.cSa has eye phenotype, enhanceable by fs(1)hG0093/fs(1)h[+]

DrefUAS.cSa has eye phenotype, enhanceable by mys[+]/mysKG02930

DrefUAS.cSa has eye phenotype, enhanceable by mys[+]/mysG0281

DrefUAS.cSa has eye phenotype, enhanceable by l(1)G0219[+]/l(1)G0219G0219

DrefUAS.cSa has eye phenotype, enhanceable by l(1)G0228G0228/l(1)G0228[+]

DrefUAS.cSa has eye phenotype, enhanceable by Trf2[+]/Trf2G0295

DrefUAS.cSa has eye phenotype, enhanceable by l(1)G0332[+]/l(1)G0332G0332

DrefUAS.cSa has eye phenotype, enhanceable by l(1)G0372[+]/l(1)G0372G0372

DrefUAS.cSa has eye phenotype, enhanceable by Trf2[+]/Trf2G0425

DrefUAS.cSa has eye phenotype, enhanceable by domeG0199b/dome[+]

DrefUAS.cSa has eye phenotype, enhanceable by Phf7KG09994/Phf7[+]

DrefUAS.cSa has eye phenotype, enhanceable by l(1)G0196[+]/l(1)G0196G0196

DrefUAS.cSa has eye phenotype, enhanceable by l(2)k08915k08915/l(2)k08915[+]

DrefUAS.cSa has eye phenotype, enhanceable by l(2)01855[+]/ds01855

DrefUAS.cSa has eye phenotype, enhanceable by Sk09530/S[+]

DrefUAS.cSa has eye phenotype, enhanceable by l(2)k06107[+]/l(2)k06107k06107

DrefUAS.cSa has eye phenotype, enhanceable by Df(2L)Prl/+

DrefUAS.cSa has eye phenotype, enhanceable by Df(2L)b83d29a/+

DrefUAS.cSa has eye phenotype, enhanceable by Df(2L)H20/+

DrefUAS.cSa has eye phenotype, enhanceable by Df(2L)TW50/+

DrefUAS.cSa has eye phenotype, enhanceable by pigeon[+]/pigeonP1

DrefUAS.cSa has eye phenotype, enhanceable by Df(2L)pr-A16/+

DrefUAS.cSa has eye phenotype, enhanceable by Df(2R)Pu-D17/+

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, enhanceable by Dll9/Dll[+]

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, enhanceable by Dll5/Dll[+]

NOT Enhanced by
Statement
Reference

DrefUAS.cSa has eye phenotype, non-enhanceable by dpyOVLR/dp[+]

DrefUAS.cSa has eye phenotype, non-enhanceable by dpyD/dp[+]

Suppressed by
Statement
Reference

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible | partially by bsk1/bsk[+]

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible | partially by bsk2/bsk[+]

DrefUAS.cSa has eye phenotype, suppressible | partially by p53[+]/p53-ns

DrefUAS.cSa has eye phenotype, suppressible by p53-ns/p53-ns

DrefUAS.cSa has eye phenotype, suppressible by Rab6[+]/Rab6k13606

DrefUAS.cSa has eye phenotype, suppressible by l(2)k05448k05448/l(2)k05448[+]

DrefUAS.cSa has eye phenotype, suppressible by +/Df(2L)TE35D-2

DrefUAS.cSa has eye phenotype, suppressible by Cul306430/Cul-3[+]

DrefUAS.cSa has eye phenotype, suppressible by +/Df(2L)TE35D-13

DrefUAS.cSa has eye phenotype, suppressible by lace[+]/lacek05305

DrefUAS.cSa has eye phenotype, suppressible by J29[+]/J29J29

DrefUAS.cSa has eye phenotype, suppressible by Cyt-c-dbln1/Cyt-c-d[+]

DrefUAS.cSa has eye phenotype, suppressible by RpS2604553/RpS26[+]

DrefUAS.cSa has eye phenotype, suppressible by l(2)k07219k07219/l(2)k07219[+]

DrefUAS.cSa has eye phenotype, suppressible by Df(2R)PC4/+

DrefUAS.cSa has eye phenotype, suppressible by stau[+]/staury9

DrefUAS.cSa has eye phenotype, suppressible by Hsf03091/Hsf[+]

DrefUAS.cSa has eye phenotype, suppressible by l(2)08717[+]/MFS1408717

DrefUAS.cSa has eye phenotype, suppressible by l(2)03050[+]/l(2)0305003050

DrefUAS.cSa has eye phenotype, suppressible by Xbp1[+]/Xbp1k13803

DrefUAS.cSa has eye phenotype, suppressible by CG10496[+]/TAF1C-like07128a

DrefUAS.cSa has eye phenotype, suppressible by MESK2k00119/MESK2[+]

DrefUAS.cSa has eye phenotype, suppressible by HP6[+]/HP6BG01429

DrefUAS.cSa has eye phenotype, suppressible by Df(1)sqh/+

DrefUAS.cSa has eye phenotype, suppressible by schlankG0365/schlank[+]

DrefUAS.cSa has eye phenotype, suppressible by l(1)G0287[+]/l(1)G0287G0287

DrefUAS.cSa has eye phenotype, suppressible by +/Df(1)16-2-19

DrefUAS.cSa has eye phenotype, suppressible by sw+/swKG05547

DrefUAS.cSa has eye phenotype, suppressible by l(1)G0004G0004/l(1)G0004[+]

DrefUAS.cSa has eye phenotype, suppressible by Df(2L)al/+

DrefUAS.cSa has eye phenotype, suppressible by l(2)k06506[+]/l(2)k06506k06506

DrefUAS.cSa has eye phenotype, suppressible by l(2)k07308k07308/l(2)k07308[+]

DrefUAS.cSa has eye phenotype, suppressible by Df(2L)S3/+

DrefUAS.cSa has eye phenotype, suppressible by l(2)10685k05810/l(2)10685[+]

DrefUAS.cSa has eye phenotype, suppressible by Df(2L)cl-h3/+

DrefUAS.cSa has eye phenotype, suppressible by Kdm5k06801/lid[+]

DrefUAS.cSa has eye phenotype, suppressible by eIF-4a[+]/eIF4Ak01501

DrefUAS.cSa has eye phenotype, suppressible by eIF-4a[+]/eIF4A02439

DrefUAS.cSa has eye phenotype, suppressible by crol[+]/crolk05205

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible | partially by osa2/osa[+]

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible | partially by mor[+]/mor1

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible | partially by osa00090/osa[+]

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible | partially by eIF-4a[+]/eIF4A02439

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible by skpA[+]/SkpA1

DrefUAS.cSa has eye phenotype, suppressible by Scer\GAL4GMR.PS

DrefUAS.cSa, Scer\GAL4en-e16E has wing vein L5 | distal phenotype, suppressible by phl[+]/RafE1

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible by E2f[+]/E2f191

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible by E2f[+]/E2f107172

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible by E2f[+]/E2f1rM729

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible by brm[+]/brm2

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible by osa2/osa[+]

DrefUAS.cSa, Scer\GAL4GMR.PS has eye phenotype, suppressible by mor[+]/mor1

NOT suppressed by
Statement
Reference

DrefUAS.cSa has eye phenotype, non-suppressible by HP6[+]/HP6BG01429rev

DrefUAS.cSa has eye phenotype, non-suppressible by dpyOVLR/dp[+]

DrefUAS.cSa has eye phenotype, non-suppressible by dpyD/dp[+]

NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The rough eye phenotype caused by expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS is weakly but significantly suppressed by bsk1/+ and by bsk2/+.

The rough eye phenotype caused by expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS is suppressed by p53-ns/+ and by p53-ns/p53-ns (with stronger suppression in the homozygous background).

Flies heterozygous for osa2, osa00090 or mor1 show suppression of the Scer\GAL4GMR.PS, DrefScer\UAS.cSa severe rough eye phenotype; eyes are modestly rough and fused ommatidia are not apparent.

Expression of bibdsRNA.Scer\UAS partially suppresses the ectopic scutellar macrochaetae, wing notching and reduction in distance between the L3 and L4 veins seen when DrefScer\UAS.cSa is expressed under the control of Scer\GAL4ptc-559.1.

The rough eye phenotype caused by expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS is partially suppressed by eIF-4a02439/+.

skpA1/+ suppresses the rough eye phenotype caused by expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS.

A Catn1/+ background suppresses the rough eye phenotype observed when DrefScer\UAS.cSa is expressed in the developing eye under the control of Scer\GAL4GMR.PS.

phlE1/+ significantly suppresses the extra vein formation seen in flies expressing DrefScer\UAS.cSa under the control of Scer\GAL4en-e16E.

Co-expression of MlfScer\UAS.cKa with DrefScer\UAS.cSa in the eye under the control of Scer\GAL4GMR.PF does not suppress the eye degeneration observed upon expression of DrefScer\UAS.cSa alone.

The rough eye phenotype seen upon expression of DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS is suppressed with co-expression of thGMR.PH or Iap2GMR.PH.

The rough eye phenotype seen upon expression of DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS is suppressed in an E2f91, E2f07172, or E2frM729 heterozygous background.

A brm2/+, osa2/+, or mor1/+ background significantly suppresses the rough eye phenotype found in flies expressing DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS.

An E(Pc)1 background weakly suppresses the rough eye phenotype found in flies expressing DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS.

Genetic crossing of the DrefScer\UAS.cSa expressing strain with Dll9 results in a weakly enhanced rough eye phenotype.

Genetic crossing of the DrefScer\UAS.cSa expressing strain with Dll5 results in a severe small eye phenotype.

Co-expression of MlfScer\UAS.cOa at least partially rescues the rough eye phenotype caused by expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS.

Xenogenetic Interactions
Statement
Reference

Co-expression of DrefScer\UAS.cSa with Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 in the eye under the control of Scer\GAL4GMR.PF does not suppress the eye degeneration observed upon expression of Zzzz\CAG63Q.Scer\UAS.T:Ivir\HA1 alone.

The rough eye phenotype seen upon expression of DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS is suppressed by co-expression of BacA\p35GMR.PH. Horizontal sections of eyes of adult flies shows that DrefScer\UAS.cSa-induced eye degeneration is partially suppressed by BacA\p35GMR.PH expression.

Complementation and Rescue Data
Comments

Co-expression of DrefScer\UAS.cSa effectively rescues the bristle phenotypes resulting from Scer\GAL4sca-109-68-mediated expression of DrefdsRNA.h-h.Scer\UAS (insertion P{UAS-Dref.IR.h-h}15).

The lethality induced by the expression of DrefCR1.Scer\UAS under the control of Scer\GAL4hs.PB (and heat-shock) is suppressed by coexpression of DrefScer\UAS.cSa.

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Mutant
Wild-type
Stocks (0)
Notes on Origin
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External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
DrefScer\UAS.cSa
DrefUAS.cSa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Sawado
Secondary FlyBase IDs
    References (23)