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General Information
Symbol
Dmel\E2f191
Species
D. melanogaster
Name
FlyBase ID
FBal0044773
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
de2f191, dE2F91, E2F91
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C21626407T

Reported nucleotide change:

C91T

Amino acid change:

Q31term | E2f1-PA; Q31term | E2f1-PB; Q31term | E2f1-PC; Q31term | E2f1-PD; Q31term | E2f1-PE; Q31term | E2f1-PF

Reported amino acid change:

Q31term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C91T.

Amino acid replacement: Q31term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

E2f91/+ flies exhibit no wing abnormalities when raised on a low dose of aminopterin (0.5mg/kg).

Homozygous mother exhibit a dramatic increase in the amount of heterochromatic DNA in the nurse cell nuclei. E2f91 animals partially rescued by E2fhs.PD have rough eyes and are female sterile. Nurse cell heterochromatin is also increased.

E2f91/E2frM729 transheterozygous larvae, are much smaller than wild-type, 5 days after egg laying. They fail to develop into 3rd instar larvae.

E2f91/E2f164 transheterozygotes develop normally, with no obvious developmental defects. The pattern of BrdU incorporation in E2f91/E2f164 eye discs is the same as wild type. E2f91/E2frM729 transheterozygotes can develop into the late second instar or early third instar larval stage at a very slow pace (around 13 days after egg laying), but do not develop into pupae. BrdU incorporation is not seen in the optic lobe region of the brain in day 13 E2f91/E2frM729 larvae, in contrast to wild-type third instar larvae. The eye discs of E2f91/E2frM729 larvae are very small and have only a few cells labelled with BrdU with no specific pattern.

E2fi1/E2f91 flies produce thin eggshells.

Enhances the position effect variegation at the w locus caused by In(1)wm4. Does not enhance the position effect variegation at the bw locus caused by In(2R)bwVDe2 or Byron or the position effect variegation of w seen in the P{wAR}41A insertion.

E2f1/Dp-dependent G[[1]]-S transcription is disrupted late in embryogenesis, but S phase takes place. Lethality of E2f191/E2f107172 occurs at late larval, early pupal stages. Larval growth is dramatically delayed taking 288 to 432 hours from larvae to pupation compared to 120 hours for wild type. Five days after egg laying larvae are very sluggish and much smaller in size than wild type. Salivary glands, imaginal discs and the brain are greatly reduced in size in 5 day old larvae but they do grow to wild type proportions by the end of their larval life. Larvae also exhibit melanotic pseudotumours, they first appear 7 days after egg laying and they grow and darken as the larvae age.

The five cell body array of the pentascolopidial chordotonal organ is disrupted.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

E2f1i1/E2f191 has lethal phenotype, enhanceable by CycE[+]/CycE05206

E2f191/E2f107172 has lethal phenotype, enhanceable | maternal effect by CycE[+]/CycE05206

Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

E2f[+]/E2f191 is an enhancer of visible phenotype of sdETX4

E2f191 is an enhancer of visible | recessive phenotype of CycEJP

NOT Enhancer of
Statement
Reference

E2f[+]/E2f191 is a non-enhancer of visible | conditional | dominant phenotype of vgnull

Suppressor of
NOT Suppressor of
Statement
Reference

E2f[+]/E2f191 is a non-suppressor of visible phenotype of DrefUAS.cSa, Scer\GAL4en-e16E

Other
Phenotype Manifest In
Suppressed by
Statement
Reference

E2f191/E2f1rM729 has eye disc phenotype, suppressible by Rbf14/Rbf120a

E2f191/E2f1rM729 has eye disc & mitotic cell cycle phenotype, suppressible by Rbf14/Rbf120a

Enhancer of
Statement
Reference

E2f[+]/E2f191 is an enhancer of wing margin phenotype of sdETX4

E2f191 is an enhancer of eye phenotype of CycEJP

NOT Enhancer of
Statement
Reference

E2f[+]/E2f191 is a non-enhancer of wing | conditional phenotype of vgnull

Suppressor of
Statement
Reference

E2f[+]/E2f191 is a suppressor | partially of follicle cell phenotype of E2f21-188, E2f2329

E2f1[+]/E2f191 is a suppressor of wing phenotype of Mmus\Cul1UAS.cHa, Scer\GAL4Bx-MS1096

E2f[+]/E2f191 is a suppressor of eye phenotype of DrefUAS.cSa, Scer\GAL4GMR.PS

E2f1164/E2f191 is a suppressor of scutellar bristle phenotype of Rbf14

E2f1164/E2f191 is a suppressor of eye phenotype of Rbf14

E2f1164/E2f191 is a suppressor of ommatidium phenotype of Rbf14

NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The reduced size of the posterior compartment of the wing that is seen in animals expressing crolScer\UAS.cMa under the control of Scer\GAL4en-e16E is suppressed if they are also heterozygous for E2f91.

vgnull/+, E2f91/+ flies display small notches at the wing tip when raised on a low dose of aminopterin (0.5mg/kg).

sdETX4/Y, E2f91/+ males display a severe mutant wing phenotype compared to sdETX4/Y males.

The addition of E2f91 suppresses the overproliferation phenotype seen in scrib1, Ras85DV12.Scer\UAS, Scer\GAL4αTub84B.PL clones.

The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2329/E2f21-188 egg chambers is partially suppressed by E2f91/+; 55% of the double mutant egg chambers show a 50% reduction in the number of follicle cells exhibiting ectopic genomic replication compared to the single mutant egg chambers.

The addition of E2f276Q1/Df(2L)G5.1 to E2f91/E2frM729 flies almost completely suppresses the E2f91/E2frM729 phenotypes - mutants develop normally without any significant delay in larval growth, reach pupal stage and finally die as mid- or late pupae. The pattern of DNA synthesis in eye discs is largely normal. The addition of Dpa2/Df(2R)vg-B to E2f91/E2frM729 flies almost completely suppresses the E2f91/E2frM729 phenotypes - mutants develop normally without any significant delay in larval growth.

The rough eye phenotype seen upon expression of DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS is suppressed in an E2f91, heterozygous background.

The early larval lethality of Rbf14/Y males is partially suppressed by E2f91/E2f164; these larvae survive to the third larval instar stage. Their development is delayed by about 2 days compared to wild type, and most of the larvae can develop further into pupae. The Rbf14/Y ; E2f91/E2f164 larvae and pupae are slightly smaller than wild type. Some develop into very late stage pupae with adult structures (such as eyes, legs, wings, cuticle and bristles) but they cannot completely eclose. There are two differences in the pattern of BrdU incorporation in the eye discs of Rbf14/Y ; E2f91/E2f164 larvae compared to wild type. Firstly, the G1 arrested region in the morphogenetic furrow is much narrower, cells just anterior to the morphogenetic furrow are not arrested in G1, instead they become part of the first mitotic wave. Secondly, some cells posterior to the second mitotic wave still incorporate BrdU. Rbf14/Y ; E2f91/E2f164 eye discs are slightly smaller than normal. There is increased cell death (seen just anterior to the morphogenetic furrow) in these eye discs compared to those of wild-type or E2f91/E2f164 larvae. Adult Rbf14 flies rescued by expressing Rbfhs.PD during the first 3 days after egg laying show a number of alterations in adult structures such as abnormal macrochaetae on the notum and rough eyes. These adult phenotypes are also rescued without heat shock if the flies are also carrying E2f91/E2f164. The larval lethality of E2f91/E2frM729 animals can be suppressed by Rbf120a/Rbf14; the larvae develop into large late third instar larvae and many can pupate. Some Rbf120a/Rbf14 ; E2f91/E2frM729 animals can develop into pharate adults with adult eyes, legs and wing structures. The development of these larvae is significantly retarded compared to wild-type larvae, with the earliest pupae observed at around day 11 after egg laying. The normal pattern of DNA replication in the optic lobe region is restored in E2f91/E2frM729 larvae that are also carrying Rbf120a/Rbf14. In Rbf120a/Rbf14 ; E2f91/E2frM729 eye discs the overall pattern of DNA replication is normal. No significant cell death is seen in these eye discs. In late third instar discs there are about 25 rows of developing photoreceptor cells (as in wild-type discs) but there are much fewer cells anterior to the morphogenetic furrow than in wild type.

One copy of CycE05206 has no effect on the viability of E2fi2/E2f91 flies. One copy of CycE05206 enhances the lethality of E2fi1/E2f91 flies; no adults are recovered.

Dominantly enhances the rough eye phenotype and reduction in the number of cells entering S phase posterior to the morphogenetic furrow seen in CycEJP homozygotes.

Lethality of E2f191/E2f107172 transheterozygotes is dramatically enhanced by reducing the maternal dosage of CycE (CycE05206).

Xenogenetic Interactions
Statement
Reference

The rough-eye phenotype observed in animals expressing Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed in a E2f91/+ background.

A E2f191/+ background rescues wing size to wild-type in Mmus\Cul1Scer\UAS.cHa/Scer\GAL4Bx-MS1096 over-expression mutants.

Complementation and Rescue Data
Rescued by
Partially rescued by

E2f191 is partially rescued by E2f1hs.PD

Comments

Mild heat induced expression of E2fhs.PS can rescue homozygous lethality.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

N. Azpiazu and M. Frasch.

Comments
Comments

Chromosome causes embryonic lethality due to a mutation other than E2f91.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (32)