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General Information
Symbol
Dmel\E2f2329
Species
D. melanogaster
Name
FlyBase ID
FBal0134271
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Excision deletion extending from the P{lacW}Mpp6k16402a insertion site to 11bp after the E2f2 stop codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{lacW} element resulting in a deletion with one breakpoint at the site of the P{lacW} insertion and the other 11bp downstream of the E2f2 stop codon, near the end of the E2f2 transcription unit.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

E2f276Q1/E2f2329 Mpp6+t4 animals have a pattern of irradiation induced apoptosis in the late third instar wing disc which is identical to that seen in wild-type animals. (Mpp6+t4 is present to rescued loss of Mpp6 in Df(2L)E2f2329.

Nearly all follicle cells in stage 13 E2f2329/E2f21-188 egg chambers show a phenotype of ectopic genomic replication, as assayed by BrdU incorporation. This phenotype is fully penetrant.

10% of eggs laid by E2f2329 females (Df(2L)E2f2329 homozygotes with Mpp6 function rescued by Mpp6+t4) hatch. 97% of the eggs laid by E2f2329 females have chorion defects. The chorions are more translucent than normal, indicating that the eggshell is thin. In stage 10B through 13 egg chambers of E2f2329 females (Df(2L)E2f2329 homozygotes with Mpp6 function rescued by Mpp6+t4), BrdU incorporation is seen throughout the entire follicle cell nuclei, rather than in characteristic subnuclear foci (which is seen in wild type). The follicle cells do not undergo an additional endocycle in these females. The number of BrdU positive follicle cell nuclei in pre-stage 10B egg chambers of these females is similar to wild type. E2f2329 males (Df(2L)E2f2329 homozygotes with Mpp6 function rescued by Mpp6+t4) are fully fertile. 8% of eggs laid by E2f2329/Df(2L)DS8 females carrying E2f21-188 (Df(2L)E2f2329/Df(2L)DS8 females carrying E2f21-188 with Mpp6 function rescued by Mpp6+t1-188) hatch. 93% of the eggs laid by these females have chorion defects. In stage 10B through 13 egg chambers of E2f2329/Df(2L)DS8 females carrying E2f21-188 (Df(2L)E2f2329/Df(2L)DS8 females carrying E2f21-188 with Mpp6 function rescued by Mpp6+t1-188), BrdU incorporation is seen throughout the entire follicle cell nuclei, rather than in characteristic subnuclear foci (which is seen in wild type). The follicle cells do not undergo an additional endocycle in these females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference

E2f21-188, E2f2329 has follicle cell phenotype, suppressible | partially by Orc2[+]/Orc2γ3

E2f21-188, E2f2329 has follicle cell phenotype, suppressible | partially by Mcm2rL074/Mcm2[+]

E2f21-188, E2f2329 has follicle cell phenotype, suppressible | partially by Orc51/Orc5[+]

E2f21-188, E2f2329 has follicle cell phenotype, suppressible | partially by E2f[+]/E2f191

Additional Comments
Genetic Interactions
Statement
Reference

An E2f2329 mutant background dominantly enhances the Scer\GAL4GMR.PF>lin-52GD12885 eye phenotype. This is accompanied by a corresponding increase in hid protein and elevated apoptosis in the eye-antennal disc.

The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2329/E2f21-188 egg chambers is partially suppressed by Orc2γ3/+ or Mcm2rL074/+; there is an approximate 65% reduction in the number of follicle cells within an egg chamber that display ectopic genomic replication and there is an apparent reduction in the intensity of ectopic BrdU incorporation.

The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2329/E2f21-188 egg chambers is partially suppressed by Orc51; the phenotype is suppressed in 36% of egg chambers.

The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2329/E2f21-188 egg chambers is partially suppressed by E2f91/+; 55% of the double mutant egg chambers show a 50% reduction in the number of follicle cells exhibiting ectopic genomic replication compared to the single mutant egg chambers.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

E2f2+t6.4 rescues the chorion defects of eggs derived from E2f2329 females (Df(2L)E2f2329 homozygotes with Mpp6 function rescued by Mpp6+t4). The follicle cell BrdU incorporation of these females is also rescued by E2f2+t6.4.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)