FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\E2f2
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General Information
Symbol
Dmel\E2f2
Species
D. melanogaster
Name
E2F transcription factor 2
Annotation Symbol
CG1071
Feature Type
protein_coding_gene
FlyBase ID
FBgn0024371
Gene Model Status
Current
Stock Availability
19 publicly available
Gene Summary
E2F transcription factor 2 (E2f2) encodes a transcription factor that belongs to the E2F gene family. It is a subunit of the DREAM complex, where it forms a heterodimer with the product of Dp to bind to DNA and repress transcription. Among their trancription targets are cell cycle genes and differentiation related genes. [Date last reviewed: 2018-09-20] (FlyBase Gene Snapshot)
All Summaries
Also Known As

dE2F2, E2f

Key Links
Genomic Location
Cytogenetic map
39A5-39A6
Sequence location
Recombination map
2-54
RefSeq locus
NT_033779 REGION:21154293..21156749
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (24 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
contributes_to DNA-binding transcription repressor activity, RNA polymerase II-specific
inferred from physical interaction with FLYBASE:Rbf2; FB:FBgn0038390
(Stevaux et al., 2002)
enables protein binding
inferred from physical interaction with UniProtKB:Q24472
(Frolov et al., 2001)
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding
inferred from mutant phenotype
(Kim et al., 2018)
inferred from direct assay
(Lee et al., 2012)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables DNA-binding transcription factor activity, RNA polymerase II-specific
inferred from biological aspect of ancestor with PANTHER:PTN000249624
(GO Reference Genome Project, 2011-)
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000249624
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR015633
(InterPro Project Members, 2004-)
Biological Process (12 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in DNA endoreduplication
inferred from mutant phenotype
(Weng et al., 2003)
inferred from genetic interaction with FLYBASE:CycE; FB:FBgn0010382
(Weng et al., 2003)
involved_in negative regulation of apoptotic process
inferred from direct assay
(Wichmann et al., 2010)
involved_in negative regulation of DNA replication
inferred from mutant phenotype
(Cayirlioglu et al., 2003, Cayirlioglu et al., 2001)
involved_in negative regulation of DNA-templated transcription
inferred from direct assay
(Taylor-Harding et al., 2004, Sawado et al., 1998)
involved_in negative regulation of G1/S transition of mitotic cell cycle
inferred from direct assay
(Stevaux et al., 2002)
involved_in negative regulation of transcription by RNA polymerase II
inferred from mutant phenotype
(Korenjak et al., 2004, Lewis et al., 2004)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
involved_in endomitotic cell cycle
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in G1/S transition of mitotic cell cycle
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in imaginal disc development
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in negative regulation of DNA-templated transcription
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in oogenesis
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in regulation of DNA-templated transcription
inferred from electronic annotation with InterPro:IPR003316
(InterPro Project Members, 2004-)
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000249624
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR015633
(InterPro Project Members, 2004-)
Cellular Component (7 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
located_in chromatin
inferred from direct assay
(Korenjak et al., 2004)
part_of DRM complex
inferred from direct assay
(Beall et al., 2007)
part_of Myb complex
inferred from direct assay
(Korenjak et al., 2004, Lewis et al., 2004)
is_active_in nucleus
inferred from direct assay
(Beall et al., 2007)
located_in nucleus
inferred from high throughput direct assay
(Sarov et al., 2016)
part_of Rb-E2F complex
inferred from direct assay
(Stevaux et al., 2002)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of RNA polymerase II transcription regulator complex
inferred from biological aspect of ancestor with PANTHER:PTN000249624
(GO Reference Genome Project, 2011-)
part_of transcription regulator complex
inferred from electronic annotation with InterPro:IPR003316
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the E2F/DP family. (O77051)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
E2F transcription factor 2 (E2f2) encodes a transcription factor that belongs to the E2F gene family. It is a subunit of the DREAM complex, where it forms a heterodimer with the product of Dp to bind to DNA and repress transcription. Among their trancription targets are cell cycle genes and differentiation related genes. [Date last reviewed: 2018-09-20]
Gene Group (FlyBase)
E2F TRANSCRIPTION FACTORS -
The E2F/DP family of transcription factors are sequence-specific DNA-binding proteins that regulate transcription. The functional E2F are transcriptional heterodimers composed of two subunits, E2F and DP. (Adapted from FBrf0128583).
DREAM TRANSCRIPTIONAL REPRESSOR COMPLEX, RBF VARIANT -
The DREAM complex is a MuvB-type transcriptional repressor complex containing multiple site-specific DNA-binding proteins, including E2f2 and Rbf. (Adapted from PMID:35468940 and FBrf0258562.)
DREAM TRANSCRIPTIONAL REPRESSOR COMPLEX, RBF2 VARIANT -
The DREAM complex is a MuvB-type transcriptional repressor complex containing multiple site-specific DNA-binding proteins, including E2f2 and Rbf2. (Adapted from PMID:35468940 and FBrf0258562.)
Protein Function (UniProtKB)
Transcriptional repressor that binds to E2f sites and represses E2f-regulated target genes. Binding to E2f sites requires transcription factor Dp. Acts synergistically with Rbf2 to antagonize E2f1-mediated transcriptional activation. Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. The DREAM complex is required for recruiting E2f2 at differentiation-specific promoters and for stabilizing E2f2-Rbf complexes during S phase. During development, the complex represses transcription of developmentally controlled E2f target genes. During oogenesis, plays a role in restricting DNA synthesis to sites of chorion gene amplification in late stage ovarian follicle cells. Plays an inhibitory role in ionizing radiation (IR)-induced p53-independent apoptosis. May be involved in cell cycle exit by temporarily limiting CycE-dependent activation of E2f-regulated transcription.
(UniProt, O77051)
Summary (Interactive Fly)

negatively regulates genes involved in the S (DNA synthesis) phase of the cell cycle - Retinoblastoma family 2 is required for the tissue-specific repression of dE2F2 target genes

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\E2f2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O77051)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
E2f2-RA
FBtr0081501
NM_058007
1485
370
E2f2-RB
FBtr0346451
NM_001299186
2002
370
Additional Transcript Data and Comments
Reported size (kB)

1.4 (northern blot)

(Sawado et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
E2f2-PA
FBpp0081029
41.4
370
4.81
O77051
NP_477355
AAF53965
E2f2-PB
FBpp0312101
41.4
370
4.81
O77051
NP_001286115
AHN54629
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

370 aa isoforms: E2f2-PA, E2f2-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

370 (aa); 41.4 (kD predicted)

(Sawado et al., 1998)
Comments
External Data
Subunit Structure (UniProtKB)

Forms a heterodimer with Dp. Interacts with Rbf/Rbf1 and Rbf2. Component of the DREAM complex, which is at least composed of Myb, Caf1-55, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, HDAC1/Rpd3 and l(3)mbt.

(UniProt, O77051)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\E2f2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.28

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 3 -- 4
organism

Comment: maternally supplied

(Cayirlioglu et al., 2001)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 5 -- 10
cell

Comment: mitotically active cells

(Cayirlioglu et al., 2001)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
Malpighian tubule primordium
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
embryonic stage 12 -- 14
neuroblast

Comment: mitotically active cells

(Cayirlioglu et al., 2001)
embryonic/larval midgut primordium

Comment: endoreplicating cells

(Cayirlioglu et al., 2001)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic Malpighian tubule
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
larval visceral muscle cell
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Sawado et al., 1998)
adult stage
ovary
(Cayirlioglu et al., 2001)
Additional Descriptive Data

E2f2 transcript is detected in all developmental stages, with highest levels in 4-8 hr embryos, and low levels in unfertilized eggs, larvae, pupae, and adults.

(Sawado et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in chromatin
inferred from direct assay
(Korenjak et al., 2004)
part_of DRM complex
inferred from direct assay
(Beall et al., 2007)
part_of Myb complex
inferred from direct assay
(Korenjak et al., 2004, Lewis et al., 2004)
is_active_in nucleus
inferred from direct assay
(Beall et al., 2007)
located_in nucleus
inferred from high throughput direct assay
(Sarov et al., 2016)
part_of Rb-E2F complex
inferred from direct assay
(Stevaux et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\E2f2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 21 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of E2f2
Transgenic constructs containing regulatory region of E2f2
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
partially lethal
partially lethal - majority live (with Df(2L)pr-A14)
partially lethal - majority live (with Df(2L)TW1)
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with E2f21-188
viable, with E2f2329
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female semi-sterile, with E2f2329/Df(2L)DS8
female semi-sterile, with E2f2k16402a/Df(2L)DS8
female semi-sterile (with Df(2L)DS8), with E2f21-188
female semi-sterile | recessive
fertile
male fertile
semi-fertile
Other Phenotypes
Allele
visible, with Scer\GAL4GMR.PF
wild-type
Phenotype manifest in
Allele
egg chorion
egg chorion, with Df(2L)DS8, E2f21-188
egg chorion, with Df(2L)DS8, E2f2329
egg chorion, with Df(2L)DS8, E2f2k16402a
egg chorion, with E2f2329/Df(2L)DS8
egg chorion, with E2f2k16402a/Df(2L)DS8
egg chorion (with Df(2L)DS8), with E2f21-188
eye, with Scer\GAL4GMR.PF
follicle cell, with E2f21-188
follicle cell, with E2f2329
follicle cell & nucleus
follicle cell & nucleus, with Df(2L)DS8, E2f21-188
follicle cell & nucleus, with Df(2L)DS8, E2f2329
follicle cell & nucleus, with Df(2L)DS8, E2f2k16402a
follicle cell & nucleus, with E2f2329/Df(2L)DS8
follicle cell & nucleus, with E2f2k16402a/Df(2L)DS8
follicle cell & nucleus (with Df(2L)DS8), with E2f21-188
ommatidium, with Scer\GAL4GMR.PF
salivary gland & nucleus (with E2f276Q1)
salivary gland & nucleus (with E2f2p111-5)
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (10)
Hsap\E2F5
6 of 14
Yes
Yes
1  
Hsap\E2F2
5 of 14
No
No
1  
Hsap\E2F4
5 of 14
No
Yes
1  
Hsap\E2F1
4 of 14
No
No
1  
Hsap\E2F3
4 of 14
No
No
2  
Hsap\E2F6
4 of 14
No
No
Hsap\E2F7
2 of 14
No
Yes
Hsap\E2F8
2 of 14
No
Yes
Hsap\TFDP1
1 of 14
No
No
Hsap\TFDP3
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (9)
Rnor\E2f4
5 of 14
Yes
Yes
Rnor\E2f5
5 of 14
Yes
Yes
Rnor\E2f1
4 of 14
No
No
Rnor\E2f2
4 of 14
No
No
Rnor\E2f3
4 of 14
No
No
Rnor\E2f6
4 of 14
No
No
Rnor\E2f7
2 of 14
No
Yes
Rnor\E2f8
1 of 14
No
Yes
Rnor\Tfdp1
1 of 14
No
No
Mus musculus (laboratory mouse) (9)
Mmus\E2f2
5 of 14
Yes
No
Mmus\E2f4
5 of 14
Yes
Yes
Mmus\E2f5
5 of 14
Yes
Yes
Mmus\E2f3
4 of 14
No
No
Mmus\E2f6
4 of 14
No
No
Mmus\E2f1
3 of 14
No
No
Mmus\E2f7
2 of 14
No
Yes
Mmus\E2f8
2 of 14
No
Yes
Mmus\Tfdp1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (9)
Xtro\e2f5
5 of 13
Yes
Yes
Xtro\e2f4
4 of 13
No
Yes
Xtro\e2f3
3 of 13
No
No
Xtro\e2f1
2 of 13
No
No
Xtro\e2f6
2 of 13
No
No
Xtro\e2f7
1 of 13
No
Yes
Xtro\rap1b
1 of 13
No
No
Xtro\timm44
1 of 13
No
No
Xtro\tprkb
1 of 13
No
Yes
Danio rerio (Zebrafish) (8)
Drer\e2f5
7 of 14
Yes
Yes
Drer\e2f4
5 of 14
No
Yes
Drer\e2f2
4 of 14
No
No
Drer\e2f3
4 of 14
No
No
Drer\e2f6
4 of 14
No
No
Drer\e2f1
3 of 14
No
No
Drer\e2f7
2 of 14
No
Yes
Drer\e2f8
1 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\efl-1
6 of 14
Yes
Yes
Cele\efl-2
3 of 14
No
Yes
Cele\efl-3
2 of 14
No
Yes
Cele\F46B6.10
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP009807
7 of 12
Yes
Yes
Agam\AgaP_AGAP001978
4 of 12
No
No
Arabidopsis thaliana (thale-cress) (6)
Atha\E2F1
7 of 13
Yes
No
Atha\ATE2F2
6 of 13
No
No
Atha\E2F3
6 of 13
No
No
Atha\DEL3
2 of 13
No
Yes
Atha\DEL1
1 of 13
No
Yes
Atha\DEL2
1 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:E2f2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
E2f1
5 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    E2F5; E2F transcription factor 5
    6 of 14
    600967
         
        E2F2; E2F transcription factor 2
        5 of 14
        600426
             
            E2F4; E2F transcription factor 4
            5 of 14
            600659
                 
                E2F1; E2F transcription factor 1
                4 of 14
                189971
                     
                    E2F3; E2F transcription factor 3
                    4 of 14
                    600427
                         
                        E2F6; E2F transcription factor 6
                        4 of 14
                        602944
                            Functional Complementation Data
                            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                            Interactions
                            Summary of Physical Interactions
                            Interaction Browsers
                            View in FlyBase Interactions Browser   View in MIST tool (external link)
                            Please see the Physical Interaction reports below for full details
                            protein-protein
                            Physical Interaction
                            Assay
                            References
                            E2f2 - Ada2b
                            anti tag coimmunoprecipitation, peptide massfingerprinting
                            (Weake et al., 2011)
                            E2f2 - Cp190
                            anti bait coimmunoprecipitation, western blot
                            (Korenjak et al., 2014)
                            E2f2 - Dp
                            anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, electrophoretic mobility shift assay, autoradiography
                            (Rhee et al., 2014, Sawado et al., 1998)
                            E2f2 - Irbp18
                            two hybrid array
                            (Shokri et al., 2019)
                            E2f2 - Myb
                            anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation
                            (Wen et al., 2008, Beall et al., 2007, Lewis et al., 2004)
                            E2f2 - Rbf
                            anti tag coimmunoprecipitation, anti tag western blot, peptide massfingerprinting, experimental knowledge based, anti bait coimmunoprecipitation, western blot, pull down, autoradiography
                            (Rhee et al., 2014, Lee et al., 2012, Nicolay et al., 2011, Acharya et al., 2010, Korenjak et al., 2004, Taylor-Harding et al., 2004)
                            E2f2 - Rbf2
                            anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, anti tag western blot
                            (Korenjak et al., 2004, Taylor-Harding et al., 2004)
                            E2f2 - Su(H)
                            two hybrid array
                            (Shokri et al., 2019)
                            E2f2 - Ubx
                            bimolecular fluorescence complementation, fluorescence microscopy
                            (Bischof et al., 2018)
                            E2f2 - lin-52
                            molecular sieving, western blot, anti tag coimmunoprecipitation
                            (Lewis et al., 2012)
                            E2f2 - mip120
                            anti bait coimmunoprecipitation, western blot
                            (Lewis et al., 2004)
                            E2f2 - mip130
                            anti bait coimmunoprecipitation, western blot
                            (Lewis et al., 2004)
                            E2f2 - mip40
                            affinity chromatography technology, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry
                            (Beall et al., 2007)
                            E2f2 - slmb
                            pull down, autoradiography
                            (Heriche et al., 2003)
                            RNA-protein
                            Physical Interaction
                            Assay
                            References
                            E2f2 - lncRNA:DREAMer
                            pull down, western blot, anti bait coimmunoprecipitation, quantitative reverse transcription pcr
                            (Li et al., 2024)
                            Summary of Genetic Interactions
                            Interaction Browsers
                            View in FlyBase Interactions Browser   View in MIST tool (external link)
                            Please look at the allele data for full details of the genetic interactions
                            Starting gene(s)
                            Interaction type
                            Interacting gene(s)
                            Reference
                            E2f2|E2f1
                            suppressible
                            CycE
                            (Weng et al., 2003)
                            E2f2
                            enhanceable
                            E2f1
                            (Weng et al., 2003, Frolov et al., 2001)
                            E2f2
                            suppressible
                            E2f1
                            (Cayirlioglu et al., 2003, Frolov et al., 2001)
                            E2f2
                            suppressible
                            Mcm2
                            (Cayirlioglu et al., 2003)
                            E2f2
                            suppressible
                            Orc2
                            (Cayirlioglu et al., 2003)
                            E2f2
                            suppressible
                            Orc5
                            (Cayirlioglu et al., 2003)
                            Starting gene(s)
                            Interaction type
                            Interacting gene(s)
                            Reference
                            E2f1
                            suppressible
                            E2f2
                            (Ambrus et al., 2007, Frolov et al., 2001)
                            gcm
                            suppressible
                            E2f2
                            (Popkova et al., 2012)
                            External Data
                            Subunit Structure (UniProtKB)
                            Forms a heterodimer with Dp. Interacts with Rbf/Rbf1 and Rbf2. Component of the DREAM complex, which is at least composed of Myb, Caf1-55, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, HDAC1/Rpd3 and l(3)mbt.
                            (UniProt, O77051 )
                            Linkouts
                            BioGRID - A database of protein and genetic interactions.
                            DroID - A comprehensive database of gene and protein interactions.
                            MIST (genetic) - An integrated Molecular Interaction Database
                            MIST (protein-protein) - An integrated Molecular Interaction Database
                            Pathways
                            Signaling Pathways (FlyBase)
                            Metabolic Pathways
                            FlyBase
                            External Links
                            External Data
                            Linkouts
                            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                            Class of Gene
                            Genomic Location and Detailed Mapping Data
                            Chromosome (arm)
                            2L
                            Recombination map
                            2-54
                            Cytogenetic map
                            39A5-39A6
                            Sequence location
                            FlyBase Computed Cytological Location
                            Cytogenetic map
                            Evidence for location
                            39A5-39A6
                            Limits computationally determined from genome sequence between P{PZ}l(2)0528705287 and P{lacW}l(2)k07215k07215&P{lacW}snRNA:U4:39Bk09410
                            Experimentally Determined Cytological Location
                            Cytogenetic map
                            Notes
                            References
                            39A-39A
                            (Huen et al., 1998)
                            Experimentally Determined Recombination Data
                            Location

                            2-54

                            (FlyBase, 2016)

                            2-54.5

                            (Comeron et al., 2012)
                            Left of (cM)
                            Right of (cM)
                            Notes
                            Stocks and Reagents
                            Stocks (19)
                            Genomic Clones (15)
                             

                            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                            cDNA Clones (77)
                             

                            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                            cDNA clones, fully sequenced
                            BDGP DGC clones
                            Other clones
                            Drosophila Genomics Resource Center cDNA clones

                            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                            cDNA Clones, End Sequenced (ESTs)
                            BDGP DGC clones
                            Other clones
                            RNAi and Array Information
                            Linkouts
                            DRSC - Results frm RNAi screens
                            Antibody Information
                            Laboratory Generated Antibodies
                             

                            polyclonal

                            (Li et al., 2024, Clavier et al., 2014, Korenjak et al., 2012, Sim et al., 2012)
                            Commercially Available Antibodies
                             
                            Cell Line Information
                            Publicly Available Cell Lines
                             
                              Other Stable Cell Lines
                               
                                Other Comments

                                E2f2 as part of the E2f-Rb complexes has a role in the down regulation of CycE during the gap phase of endocycling cells in normal development.

                                (Weng et al., 2003)

                                E2f2 mutants are viable, but females are partially sterile. Mutant follicle cells terminate endocycles normally, but then fail to confine DNA synthesis to sites of gene amplification and inappropriately begin genomic DNA replication (this ectopic DNA synthesis is not a continuation of the endocycle program).

                                (Cayirlioglu et al., 2001)
                                Relationship to Other Genes
                                Source for database merge of
                                Additional comments
                                Nomenclature History
                                Source for database identify of

                                Source for identity of: E2f2 CG1071

                                (Wortman, 1999.11)
                                Nomenclature comments
                                Etymology
                                Synonyms and Secondary IDs (11)
                                Reported As
                                Symbol Synonym
                                CG1071
                                (National Institute of Genetics Fly Stocks, 2022-, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Perkins et al., 2009, Beall et al., 2007)
                                E2F
                                (Luo et al., 2020, Heseding et al., 2017, Korenjak et al., 2012, Joe Song et al., 2009, Thacker et al., 2003)
                                E2F2
                                (Raicu et al., 2024, Raicu et al., 2024, Payankaulam et al., 2022, Yamamoto-Matsuda et al., 2022, Mouawad et al., 2020, Zhang et al., 2016, Gómez-Lamarca et al., 2014, Lim et al., 2013, modENCODE Consortium et al., 2010, Tanaka-Matakatsu et al., 2009, Wen et al., 2008, Wen et al., 2008, Beall et al., 2007, Doggett et al., 2007, Garcia et al., 2007, Georlette et al., 2007, Ullah et al., 2007, Parakati and Dimario, 2005, Heriche et al., 2003, Orian et al., 2003)
                                E2f
                                (Li and Hidalgo, 2020, Ashwal-Fluss et al., 2014, Ghavi-Helm et al., 2014, Singer et al., 2014, Bangi et al., 2011)
                                E2f2
                                (Li et al., 2024, Li et al., 2024, Peng et al., 2024, Trujillo et al., 2024, Socha et al., 2023, Baonza et al., 2022, Kang et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Cattenoz et al., 2021, Ferguson et al., 2021, Parra-Peralbo et al., 2021, Cho et al., 2020, Rust et al., 2020, Santana et al., 2020, Hall et al., 2019, Khan et al., 2019, Mouawad et al., 2019, Shokri et al., 2019, Ariss et al., 2018, Bischof et al., 2018, Gene Disruption Project members, 2018-, Transgenic RNAi Project members, 2017-, Xiang et al., 2017, Sarov et al., 2016, Baëza et al., 2015, Doggett et al., 2015, model organism Encyclopedia of Regulatory Network (modERN) Project, 2015-, Schertel et al., 2015, Blanchard et al., 2014, Luck et al., 2014, Bonke et al., 2013, Debruhl et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Lewis et al., 2012, Popkova et al., 2012, Zhai et al., 2012, Davis et al., 2011, Toku et al., 2011, Weake et al., 2011, Acharya et al., 2010, Terashima and Bownes, 2005, Stanyon et al., 2004, Cayirlioglu et al., 2003)
                                dE2F-2
                                (Huen et al., 1998)
                                dE2F2
                                (Hoareau et al., 2024, Kim et al., 2018, Korenjak et al., 2014, Rhee et al., 2014, Korenjak et al., 2012, Lee et al., 2012, Lee et al., 2010, Rimkus et al., 2008, Herr et al., 2007, Lu et al., 2007, Tountas and Fortini, 2007, Rowland and Bernards, 2006, Korenjak and Brehm, 2005, Stevaux et al., 2005, Coffman, 2004, Korenjak et al., 2004, Lipsick, 2004, Taylor-Harding et al., 2004, Zraly et al., 2004, Frolov et al., 2003, Lee and Orr-Weaver, 2003, Cayirlioglu et al., 2001, Frolov et al., 2001, Cayirlioglu et al., 2000, Douglas et al., 2000, Harbour and Dean, 2000, Myster and Duronio, 2000, Sawado, 1998.8.11, Sawado et al., 1998)
                                dE2f2
                                (Bhaskar et al., 2014, Ji et al., 2012, Gordon and Du, 2011, Ji et al., 2006, Moon et al., 2005, Stevaux et al., 2005, Attwooll et al., 2004)
                                de2f2
                                (Wei et al., 2015, Korenjak et al., 2012, Ambrus et al., 2009, Longworth et al., 2008, Nicolay and Frolov, 2008, Ambrus et al., 2007, Frolov et al., 2007, Rasheva et al., 2006, Frolov et al., 2005, Moon et al., 2005, Frolov et al., 2004, Moon et al., 2004, Dimova et al., 2003, Du et al., 2003, Frolov et al., 2003, Frolov et al., 2003, Weng et al., 2003, Frolov et al., 2002, Frolov et al., 2001, Frolov et al., 2001)
                                e2f2
                                (Zhang et al., 2021, Wang et al., 2020, Miles et al., 2014, Miles et al., 2012, Rovani et al., 2012, Sim et al., 2012, Buttitta et al., 2010, Buttitta et al., 2007)
                                Name Synonyms
                                E2F transcription factor 2
                                (Davis et al., 2011, Terashima and Bownes, 2005, Huen et al., 1998)
                                Secondary FlyBase IDs
                                  Datasets (0)
                                  Study focus (0)
                                  Experimental Role
                                  Project
                                  Project Type
                                  Title
                                  Study result (0)
                                  Result
                                  Result Type
                                  Title
                                  External Crossreferences and Linkouts ( 56 )
                                  Sequence Crossreferences
                                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                                  GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                                  UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                                  UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                                  UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                                  Other crossreferences
                                  AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                                  BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                                  DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                                  EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                                  FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                                  FlyMine - An integrated database for Drosophila genomics
                                  InterPro - A database of protein families, domains and functional sites
                                  KEGG Genes - Molecular building blocks of life in the genomic space.
                                  MARRVEL_MODEL - MARRVEL (model organism gene)
                                  Linkouts
                                  BioGRID - A database of protein and genetic interactions.
                                  Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                                  DroID - A comprehensive database of gene and protein interactions.
                                  DRSC - Results frm RNAi screens
                                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                                  Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                                  Flygut - An atlas of the Drosophila adult midgut
                                  FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                                  iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                                  Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                                  KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                                  MIST (genetic) - An integrated Molecular Interaction Database
                                  MIST (protein-protein) - An integrated Molecular Interaction Database
                                  Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                                  SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                                  References (194)