FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\E2f21-188
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General Information
Symbol
Dmel\E2f21-188
Species
D. melanogaster
Name
FlyBase ID
FBal0134272
Feature type
allele
Associated gene
Dmel\E2f2
Associated Insertion(s)
Carried in Construct
P{E2f21-188}
Key Links
Transgenic product class
frameshift_truncation
(Cayirlioglu et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
frameshift_truncation
(Cayirlioglu et al., 2001)
Mutagen
in vitro construct
(Cayirlioglu et al., 2001)
Progenitor genotype
E2f2+t6.4
(Cayirlioglu et al., 2001)
Carried in construct
P{E2f21-188}
Cytology
Description

14bp deletion in E2f2, resulting in a frameshift after amino acid 188, followed by a stop codon two codons later.

(Cayirlioglu et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
E2f2
Encoded product / tool
E2f2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Nearly all follicle cells in stage 13 E2f2329/E2f21-188 egg chambers show a phenotype of ectopic genomic replication, as assayed by BrdU incorporation. This phenotype is fully penetrant.

      (Cayirlioglu et al., 2003)

      8% of eggs laid by E2f2329/Df(2L)DS8 females carrying E2f21-188 (Df(2L)E2f2329/Df(2L)DS8 females carrying E2f21-188 with Mpp6 function rescued by Mpp6+t1-188) hatch. 93% of the eggs laid by these females have chorion defects. In stage 10B through 13 egg chambers of E2f2329/Df(2L)DS8 females carrying E2f21-188 (Df(2L)E2f2329/Df(2L)DS8 females carrying E2f21-188 with Mpp6 function rescued by Mpp6+t1-188), BrdU incorporation is seen throughout the entire follicle cell nuclei, rather than in characteristic subnuclear foci (which is seen in wild type). The follicle cells do not undergo an additional endocycle in these females. 44% of eggs laid by E2f2k16402a/Df(2L)DS8 females carrying E2f21-188 (with Mpp6 function rescued by Mpp6+t1-188) hatch. 84% of the eggs laid by these females have chorion defects. 88% of the egg chambers of these females have an abnormal BrdU incorporation pattern in the follicle cell nuclei. Three types of abnormal incorporation pattern are seen; incorporation throughout the nucleus, lack of incorporation and nuclei containing both gene amplification foci and genomic incorporation.

      (Cayirlioglu et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      E2f1i2/E2f191, E2f21-188, E2f2329 has lethal phenotype

      (Cayirlioglu et al., 2003)
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      E2f21-188, E2f2329 has follicle cell phenotype, suppressible | partially by Orc2[+]/Orc2γ3

      (Cayirlioglu et al., 2003)

      E2f21-188, E2f2329 has follicle cell phenotype, suppressible | partially by Mcm2rL074/Mcm2[+]

      (Cayirlioglu et al., 2003)

      E2f21-188, E2f2329 has follicle cell phenotype, suppressible | partially by Orc51/Orc5[+]

      (Cayirlioglu et al., 2003)

      E2f21-188, E2f2329 has follicle cell phenotype, suppressible | partially by E2f[+]/E2f191

      (Cayirlioglu et al., 2003)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2329/E2f21-188 egg chambers is partially suppressed by Orc2γ3/+ or Mcm2rL074/+; there is an approximate 65% reduction in the number of follicle cells within an egg chamber that display ectopic genomic replication and there is an apparent reduction in the intensity of ectopic BrdU incorporation.

      The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2329/E2f21-188 egg chambers is partially suppressed by Orc51; the phenotype is suppressed in 36% of egg chambers.

      The ectopic genomic replication that is seen in the follicle cells of stage 13 E2f2329/E2f21-188 egg chambers is partially suppressed by E2f91/+; 55% of the double mutant egg chambers show a 50% reduction in the number of follicle cells exhibiting ectopic genomic replication compared to the single mutant egg chambers.

      (Cayirlioglu et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      E2f21-188
      (Cayirlioglu et al., 2003)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)