FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Mcm2
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General Information
Symbol
Dmel\Mcm2
Species
D. melanogaster
Name
Minichromosome maintenance 2
Annotation Symbol
CG7538
Feature Type
protein_coding_gene
FlyBase ID
FBgn0014861
Gene Model Status
Current
Stock Availability
8 publicly available
Enzyme Name (EC)
DNA 3'-5' helicase (5.6.2.4)
Gene Summary
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. (UniProt, P49735)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

DmMCM2, MCM2-7

Key Links
Genomic Location
Cytogenetic map
84F6-84F6
Sequence location
Recombination map
3-48
RefSeq locus
NT_033777 REGION:8264970..8267924
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
contributes_to 3'-5' DNA helicase activity
inferred from direct assay
(Moyer et al., 2006)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
contributes_to 3'-5' DNA helicase activity
inferred from biological aspect of ancestor with PANTHER:PTN000179907
(GO Reference Genome Project, 2011-)
enables ATP binding
(InterPro Project Members, 2004-)
enables DNA binding
(InterPro Project Members, 2004-)
enables single-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
contributes_to single-stranded DNA helicase activity
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in chromosome condensation
inferred from mutant phenotype
(Christensen and Tye, 2003)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in DNA replication initiation
inferred from electronic annotation with InterPro:IPR008045
(InterPro Project Members, 2004-)
involved_in DNA replication
inferred from electronic annotation with InterPro:IPR018525
(InterPro Project Members, 2004-)
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in double-strand break repair via break-induced replication
inferred from biological aspect of ancestor with PANTHER:PTN000179907
(GO Reference Genome Project, 2011-)
involved_in mitotic DNA replication initiation
inferred from biological aspect of ancestor with PANTHER:PTN000179907
(GO Reference Genome Project, 2011-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of MCM complex
inferred from electronic annotation with InterPro:IPR008045
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000179712
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR008045
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MCM family. (P49735)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
3'-5' DNA helicase activity
Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction (5.6.2.4)
Summaries
Gene Group (FlyBase)
MCM2-7 COMPLEX -
The heterohexameric MCM2-7 complex is the helicase motor of the CMG (Cdc45-MCM-GINS) replicative helicase of eukaryotes. The six MCM subunits are related to one another and are members of the AAA+ (ATPases associated with diverse cellular activities) superfamily. Hydrolysis of ATP by the individual MCM subunits catalyses the conformational changes that drive unwinding of the DNA duplex. However, the eukaryotic MCM complex alone has no or little helicase activity, requiring the other components of the CMG complex (Cdc45 and the GINS complex) for activation. (Adapted from PMID:23412083.)
CMG COMPLEX -
The CMG (Cdc45-MCM-GINS) complex is the eukaryotic replicative helicase, the enzyme that unwinds double-stranded DNA at replication forks. It is a macromolecular assembly of three replication factors: the Cdc45 protein, and the MCM and GINS complexes. All three components are essential for CMG function, with the MCM2-7 complex functioning as the molecular motor that harnesses the energy of ATP hydrolysis to catalyse strand separation. (Adapted from PMID:23412083.)
MCM FAMILY HELICASES -
The MCM (minichromosome maintenance) family comprise DNA helicases sharing a common 'MCM domain'. MCM2-7 form a heterohexameric complex that is essential for initiation of DNA replication and also acts during elongation as the replicative helicase. MCM8 and MCM9 are not part of this complex - they have roles in replication elongation, meiotic recombination and DNA repair. MCM8 and 9 arose early during eukaryotic evolution, though one or both have been lost in multiple lineages: in mammals, MCM8 and MCM9 form a functional heterodimeric complex; D. melanogaster and related species possess MCM8 only; while other species (e.g. yeast, C. elegans) have neither. (Adapted from PMID:23412083, PMID:30743181 and FBrf0188222.)
Protein Function (UniProtKB)
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
(UniProt, P49735)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Mcm2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P49735)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mcm2-RA
FBtr0081827
NM_057773
2898
887
Additional Transcript Data and Comments
Reported size (kB)

2.8 (compiled cDNA)

(Treisman et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Mcm2-PA
FBpp0081317
100.4
887
4.74
P49735
NP_477121
AAF54207
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

887 (aa)

(Treisman et al., 1995)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). Interacts with Mcm10.

(UniProt, P49735)
Post Translational Modification

Phosphorylated by the catalytic component of the Dbf4-dependent kinase (DDK) complex Cdc7.

(UniProt, P49735)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.24

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
presumptive embryonic/larval peripheral nervous system
(Treisman et al., 1995)
central nervous system
(Treisman et al., 1995)
Malpighian tubule
(Treisman et al., 1995)
larval digestive system
(Treisman et al., 1995)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4
organism | ubiquitous
(Treisman et al., 1995)
embryonic stage 4 -- 6
mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 8
organism | ubiquitous
(Treisman et al., 1995)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
antennal primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
dorsal sensory complex primordium
(Fisher et al., 2012)
labial sensory complex primordium
(Fisher et al., 2012)
maxillary sensory complex primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
ventral sensory complex anlage
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
embryonic lateral cord
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
Additional Descriptive Data

Mcm2 transcripts are maternally contributed to the embryo and disappear at cellularization. They are expressed uniformly at germ band extension and are subsequently restricted to a pattern that includes continuous expression in the CNS and transient expression in a number of other tissues, including the Malpighian tubules and the gut.

(Treisman et al., 1995)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm2 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mcm2
Transgenic constructs containing regulatory region of Mcm2
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (9)
Hsap\MCM2
14 of 14
Yes
Yes
Hsap\MCM4
4 of 14
No
No
Hsap\MCM5
3 of 14
No
No
1  
Hsap\MCM6
3 of 14
No
No
Hsap\MCM7
3 of 14
No
No
Hsap\MCM8
3 of 14
No
No
Hsap\MCM9
3 of 14
No
Yes
Hsap\MCM3
2 of 14
No
No
Hsap\MCMDC2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (9)
Rnor\Mcm2
14 of 14
Yes
Yes
Rnor\Mcm4
4 of 14
No
No
Rnor\Mcm3
3 of 14
No
No
Rnor\Mcm5
3 of 14
No
No
Rnor\Mcm6
3 of 14
No
No
Rnor\Mcm7
3 of 14
No
No
Rnor\Mcm8
3 of 14
No
No
Rnor\Mcm9
2 of 14
No
Yes
Rnor\Mcmdc2
1 of 14
No
No
Mus musculus (laboratory mouse) (8)
Mmus\Mcm2
14 of 14
Yes
Yes
Mmus\Mcm4
4 of 14
No
No
Mmus\Mcm5
3 of 14
No
No
Mmus\Mcm6
3 of 14
No
No
Mmus\Mcm7
3 of 14
No
No
Mmus\Mcm8
3 of 14
No
No
Mmus\Mcm3
2 of 14
No
No
Mmus\Mcm9
2 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (12)
Xtro\mcm2
11 of 13
Yes
Yes
Xtro\mcm4
3 of 13
No
No
Xtro\mcm5
2 of 13
No
No
Xtro\mcm6.2
2 of 13
No
No
Xtro\mcm6
2 of 13
No
No
Xtro\mcm7
2 of 13
No
No
Xtro\mcm8
2 of 13
No
No
Xtro\mcm9
2 of 13
No
Yes
Xtro\zmcm3
2 of 13
No
No
Xtro\dnajc22
1 of 13
No
No
Xtro\hdlbp
1 of 13
No
No
Xtro\mcm3
1 of 13
No
Yes
Danio rerio (Zebrafish) (10)
Drer\mcm2
13 of 14
Yes
Yes
Drer\mcm4
4 of 14
No
No
Drer\mcm3
3 of 14
No
No
Drer\mcm3l
3 of 14
No
No
Drer\mcm5
3 of 14
No
No
Drer\mcm6
3 of 14
No
No
Drer\mcm6l
3 of 14
No
No
Drer\mcm8
3 of 14
No
No
Drer\mcm9
3 of 14
No
Yes
Drer\mcm7
2 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\mcm-2
13 of 14
Yes
Yes
Cele\mcm-4
4 of 14
No
No
Cele\mcm-3
3 of 14
No
No
Cele\mcm-5
3 of 14
No
No
Cele\mcm-7
3 of 14
No
No
Cele\mcm-6
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (10)
Agam\AgaP_AGAP004275
12 of 12
Yes
Yes
Agam\AgaP_AGAP012691
5 of 12
No
Yes
Agam\AgaP_AGAP004956
4 of 12
No
No
Agam\AgaP_AGAP001135
3 of 12
No
No
Agam\AgaP_AGAP001901
3 of 12
No
Yes
Agam\AgaP_AGAP002580
3 of 12
No
No
Agam\AgaP_AGAP002581
3 of 12
No
No
Agam\AgaP_AGAP004408
3 of 12
No
No
Agam\AgaP_AGAP005800
3 of 12
No
No
Agam\MCM6_ANOGA
3 of 12
No
No
Arabidopsis thaliana (thale-cress) (9)
Atha\MCM2
13 of 13
Yes
Yes
Atha\MCM4
4 of 13
No
No
Atha\MCM3
3 of 13
No
No
Atha\MCM5
3 of 13
No
No
Atha\MCM6
3 of 13
No
No
Atha\MCM8
3 of 13
No
No
Atha\MCM9
3 of 13
No
Yes
Atha\PRL
3 of 13
No
No
Atha\CHLI2
1 of 13
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\MCM2
13 of 13
Yes
Yes
Scer\MCM3
2 of 13
No
No
Scer\MCM4
2 of 13
No
No
Scer\MCM5
2 of 13
No
No
Scer\MCM6
2 of 13
No
No
Scer\MCM7
2 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\mcm2
12 of 12
Yes
Yes
Spom\mcm3
2 of 12
No
No
Spom\mcm4
2 of 12
No
No
Spom\mcm5
2 of 12
No
No
Spom\mcm6
2 of 12
No
No
Spom\mcm7
2 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\yifB
2 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Mcm2. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (7)
dpa
7 of 13
Mcm3
6 of 13
Mcm5
6 of 13
Mcm6
6 of 13
Mcm7
6 of 13
rec
6 of 13
mei-218
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 1 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MCM2; minichromosome maintenance complex component 2
    14 of 14
    116945
    MCM4; minichromosome maintenance complex component 4
    4 of 14
    602638
    MCM5; minichromosome maintenance complex component 5
    3 of 14
    602696
     
    MCM6; minichromosome maintenance complex component 6
    3 of 14
    601806
    MCM7; minichromosome maintenance complex component 7
    3 of 14
    600592
        MCM8; minichromosome maintenance 8 homologous recombination repair factor
        3 of 14
        608187
        MCM9; minichromosome maintenance 9 homologous recombination repair factor
        3 of 14
        610098
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Mcm2 - Cdc45
        anti bait coimmunoprecipitation, mass spectrometry studies of complexes, cosedimentation, electron microscopy, anti tag coimmunoprecipitation, molecular weight estimation by silver staining, anti tag western blot, western blot
        (Costa et al., 2014, Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006, Loebel et al., 2000)
        Mcm2 - Cdc7
        enzymatic study, autoradiography
        (Stephenson et al., 2015)
        Mcm2 - Ctf4
        two hybrid
        (Gosnell and Christensen, 2011)
        Mcm2 - Mcm10
        two hybrid
        (Apger et al., 2010)
        Mcm2 - Mcm3
        cosedimentation, electron microscopy, anti tag coimmunoprecipitation, western blot, pull down
        (Costa et al., 2014, Costa et al., 2011)
        Mcm2 - Mcm5
        anti bait coimmunoprecipitation, western blot, electron microscopy, experimental knowledge based, cosedimentation
        (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011, Moyer et al., 2006, Loebel et al., 2000)
        Mcm2 - Mcm6
        cosedimentation, electron microscopy, experimental knowledge based
        (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)
        Mcm2 - Mcm7
        cosedimentation, electron microscopy
        (Costa et al., 2011)
        Mcm2 - Psf1
        cosedimentation, electron microscopy
        (Costa et al., 2011)
        Mcm2 - Psf2
        anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy
        (Costa et al., 2011, Moyer et al., 2006)
        Mcm2 - Psf3
        cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot
        (Costa et al., 2011, Moyer et al., 2006)
        Mcm2 - Sld5
        cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot
        (Costa et al., 2011, Moyer et al., 2006)
        Mcm2 - dpa
        cosedimentation, electron microscopy
        (Costa et al., 2011)
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        E2f2
        suppressible
        Mcm2
        (Cayirlioglu et al., 2003)
        External Data
        Subunit Structure (UniProtKB)
        Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). Interacts with Mcm10.
        (UniProt, P49735 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-48
        Cytogenetic map
        84F6-84F6
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        84F6-84F6
        Limits computationally determined from genome sequence between P{PZ}grn05930 and P{PZ}Mcm2rL074
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        84F6-84F7
        (Treisman, 1995.9.29, BDGP Project Members, 1994-1999)
        84F6-84F7
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        Experimentally Determined Recombination Data
        Location

        3-48

        (FlyBase, 2016)

        3-44.8

        (Comeron et al., 2012)

        3-

        (Treisman, 1995.9.29)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (8)
        Genomic Clones (14)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (50)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Cell Line Information
          Publicly Available Cell Lines
           
            Other Stable Cell Lines
             
            Other Comments

            The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

            (Moyer et al., 2006)

            CycE protein can drive chromosome association of Mcm2 in polytene nuclei and DNA synthesis erases this association.

            (Su and O'Farrell, 1998)

            Physical interactions exist between three MCM proteins, dpa, Mcm2 and Mcm5. The three proteins reside predominantly in stable multi-protein complexes of about 600kD. Coimmunoprecipitation experiments indicate there are at least two distinct 600kD complexes, one of which includes Mcm5 and the other dpa.

            (Su et al., 1996)

            For the yeast genes MCM stands for 'minichromosome maintenance'.

            (Treisman et al., 1995)

            Mcm2 plays a role in the regulation of DNA replication analogous to that of its yeast counterpart (MCM2).

            (Treisman et al., 1995)
            Relationship to Other Genes
            Source for database merge of
            Additional comments
            Nomenclature History
            Source for database identify of

            Source for identity of: Mcm2 CG7538

            (Zhao, 1999.11)
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (17)
            Reported As
            Symbol Synonym
            CG7538
            (National Institute of Genetics Fly Stocks, 2023-, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Keleman et al., 2009.8.5)
            DmMCM2
            (Kearsey and Labib, 1998, Ohno et al., 1998, Su and O'Farrell, 1998, Su and O'Farrell, 1997, Su et al., 1997, Su et al., 1996, Treisman et al., 1995)
            DmMcm2
            (Loebel et al., 2000)
            DmeMCM2
            (Blanton et al., 2005)
            MCM2
            (Urban et al., 2022, Wooten et al., 2020, Hua and Orr-Weaver, 2017, Bleichert et al., 2015, Crevel et al., 2011, Apger et al., 2010, Ilves et al., 2010, Lemos et al., 2010, Moshkin et al., 2009, Crevel et al., 2007, Hong et al., 2007, Lee and Orr-Weaver, 2003, Claycomb et al., 2002, Crevel et al., 2001, Feger, 1999, Treisman, 1995.9.29)
            MCM2-7
            (Kim and Orr-Weaver, 2011, Macalpine et al., 2010)
            MCM2_DROME
            (Storm and Sonnhammer, 2003)
            McM2
            (De Lucia et al., 2005)
            Mcm2
            (Sutton et al., 2024, Khalili et al., 2023, Beaver et al., 2022, Rubio-Ferrera et al., 2022, Cahill et al., 2021, Sureka and Mishra, 2021, Kose et al., 2020, Williams et al., 2020, Burnham et al., 2019, Parker et al., 2019, Vorobyeva and Mazina, 2019, Kohzaki, 2018, Kohzaki et al., 2018, Sureka et al., 2018, Transgenic RNAi Project members, 2017-, Kong et al., 2016, Völker-Albert et al., 2016, Dequéant et al., 2015, Nikalayevich and Ohkura, 2015, Stephenson et al., 2015, Zirin et al., 2015, Costa et al., 2014, Korenjak et al., 2014, Liang et al., 2014, Luck et al., 2014, Yan et al., 2014, Gurudatta et al., 2013, Kwon et al., 2013, Moshkin et al., 2013, Schertel et al., 2013, Antao et al., 2012, Chen et al., 2012, Kalajdzic et al., 2012, Longworth et al., 2012, Costa et al., 2011, Gosnell and Christensen, 2011, Neumüller et al., 2011, Orihara-Ono et al., 2011, Frise et al., 2010, Maqbool et al., 2010, McElroy et al., 2008, Foglietti et al., 2006, Moyer et al., 2006, Loop et al., 2004, Cayirlioglu et al., 2003)
            Mcm2-7
            (Kohzaki and Murakami, 2018)
            PCR3
            (Su et al., 1997)
            dMCM2
            (Burke et al., 2001)
            dMcm2
            (Sahashi et al., 2013)
            l(3)rL074
            mcm2
            (Haines and Eisen, 2018)
            mcm2-7
            (Powell et al., 2015)
            Name Synonyms
            Minichromosome maintenance 2
            (Dequéant et al., 2015, Fernandez-Costa and Artero, 2010)
            Secondary FlyBase IDs
            • FBgn0011511
            Datasets (1)
            Study focus (1)
            Experimental Role
            Project
            Project Type
            Title
            • bait_protein
            modENCODE_replication_factor_ChIP
            Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 57 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            PDB - An information portal to biological macromolecular structures
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (146)