FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Mcm5
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General Information
Symbol
Dmel\Mcm5
Species
D. melanogaster
Name
Minichromosome maintenance 5
Annotation Symbol
CG4082
Feature Type
protein_coding_gene
FlyBase ID
FBgn0017577
Gene Model Status
Current
Stock Availability
9 publicly available
Enzyme Name (EC)
DNA 3'-5' helicase (5.6.2.4)
Gene Summary
Minichromosome maintenance 5 (Mcm5) encodes a protein that is involved in mitotic DNA replication and is required to resolve meiotic double-strand breaks into crossovers. [Date last reviewed: 2018-09-20] (FlyBase Gene Snapshot)
All Summaries
Also Known As

DmMCM5

Key Links
Genomic Location
Cytogenetic map
86C6-86C6
Sequence location
Recombination map
3-50
RefSeq locus
NT_033777 REGION:10788524..10791150
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
contributes_to 3'-5' DNA helicase activity
inferred from direct assay
(Moyer et al., 2006)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
contributes_to 3'-5' DNA helicase activity
inferred from biological aspect of ancestor with PANTHER:PTN000180161
(GO Reference Genome Project, 2011-)
enables ATP binding
(InterPro Project Members, 2004-)
enables DNA binding
inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR018525, InterPro:IPR031327
(InterPro Project Members, 2004-)
enables DNA replication origin binding
inferred from electronic annotation with InterPro:IPR008048
(InterPro Project Members, 2004-)
enables single-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
contributes_to single-stranded DNA helicase activity
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
Biological Process (7 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
involved_in chromosome condensation
inferred from mutant phenotype
(Christensen and Tye, 2003)
involved_in DNA endoreduplication
inferred from mutant phenotype
(Park and Asano, 2008)
involved_in meiotic cell cycle
inferred from mutant phenotype
(Page et al., 2007)
involved_in resolution of meiotic recombination intermediates
inferred from mutant phenotype
(Lake et al., 2007)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in DNA replication initiation
inferred from electronic annotation with InterPro:IPR008048
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000180161
(GO Reference Genome Project, 2011-)
involved_in DNA replication
inferred from electronic annotation with InterPro:IPR018525
(InterPro Project Members, 2004-)
involved_in double-strand break repair via break-induced replication
inferred from biological aspect of ancestor with PANTHER:PTN000180161
(GO Reference Genome Project, 2011-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of MCM complex
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR008048
(InterPro Project Members, 2004-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000179712
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR008048
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MCM family. (Q9VGW6)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
3'-5' DNA helicase activity
Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction (5.6.2.4)
Summaries
Gene Snapshot
Minichromosome maintenance 5 (Mcm5) encodes a protein that is involved in mitotic DNA replication and is required to resolve meiotic double-strand breaks into crossovers. [Date last reviewed: 2018-09-20]
Gene Group (FlyBase)
MCM2-7 COMPLEX -
The heterohexameric MCM2-7 complex is the helicase motor of the CMG (Cdc45-MCM-GINS) replicative helicase of eukaryotes. The six MCM subunits are related to one another and are members of the AAA+ (ATPases associated with diverse cellular activities) superfamily. Hydrolysis of ATP by the individual MCM subunits catalyses the conformational changes that drive unwinding of the DNA duplex. However, the eukaryotic MCM complex alone has no or little helicase activity, requiring the other components of the CMG complex (Cdc45 and the GINS complex) for activation. (Adapted from PMID:23412083.)
CMG COMPLEX -
The CMG (Cdc45-MCM-GINS) complex is the eukaryotic replicative helicase, the enzyme that unwinds double-stranded DNA at replication forks. It is a macromolecular assembly of three replication factors: the Cdc45 protein, and the MCM and GINS complexes. All three components are essential for CMG function, with the MCM2-7 complex functioning as the molecular motor that harnesses the energy of ATP hydrolysis to catalyse strand separation. (Adapted from PMID:23412083.)
MCM FAMILY HELICASES -
The MCM (minichromosome maintenance) family comprise DNA helicases sharing a common 'MCM domain'. MCM2-7 form a heterohexameric complex that is essential for initiation of DNA replication and also acts during elongation as the replicative helicase. MCM8 and MCM9 are not part of this complex - they have roles in replication elongation, meiotic recombination and DNA repair. MCM8 and 9 arose early during eukaryotic evolution, though one or both have been lost in multiple lineages: in mammals, MCM8 and MCM9 form a functional heterodimeric complex; D. melanogaster and related species possess MCM8 only; while other species (e.g. yeast, C. elegans) have neither. (Adapted from PMID:23412083, PMID:30743181 and FBrf0188222.)
Protein Function (UniProtKB)
Acts as a component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
(UniProt, Q9VGW6)
Summary (Interactive Fly)

AAA+ ATPase family - required in the Mcm complex for licensing and initiating origins of replication - it acts during elongation as a helicase at the replication forks - acts in meiosis in maturation of double-strand breaks into crossovers

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Mcm5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VGW6)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mcm5-RA
FBtr0082279
NM_079584
2452
733
Additional Transcript Data and Comments
Reported size (kB)

2.2 (longest cDNA)

(Su et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Mcm5-PA
FBpp0081756
82.3
733
7.91
Q9VGW6
NP_524308
AAF54557
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

732 (aa)

(Su et al., 1997)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).

(UniProt, Q9VGW6)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm5 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.33

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | intense | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | intense | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
organism | intense | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 9 -- 12
midline primordium | subset
(Kearney et al., 2004)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
embryonic neuroblast of ventral nerve cord primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
midline primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
procephalic neuroblast
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain surface glial cell
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
larval lateral VNC surface glial cell
(Fisher et al., 2012)
mushroom body primordium

Comment: reported as embryonic central brain mushroom body

(Fisher et al., 2012)
ventral midline of embryo
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
embryonic stage 17
gonad

Comment: soma and germline

(Casper and Van Doren, 2009)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm5 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 17 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mcm5
Transgenic constructs containing regulatory region of Mcm5
Aberrations (Deficiencies and Duplications) ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (9)
Hsap\MCM5
14 of 14
Yes
Yes
1  
Hsap\MCM2
3 of 14
No
No
Hsap\MCM4
3 of 14
No
No
Hsap\MCM6
3 of 14
No
No
Hsap\MCM7
3 of 14
No
No
Hsap\MCM8
3 of 14
No
No
Hsap\MCM9
3 of 14
No
Yes
Hsap\MCM3
2 of 14
No
No
Hsap\MCMDC2
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (9)
Rnor\Mcm5
14 of 14
Yes
Yes
Rnor\Mcm2
3 of 14
No
No
Rnor\Mcm3
3 of 14
No
No
Rnor\Mcm4
3 of 14
No
No
Rnor\Mcm6
3 of 14
No
No
Rnor\Mcm7
3 of 14
No
No
Rnor\Mcm8
3 of 14
No
No
Rnor\Mcm9
1 of 14
No
No
Rnor\Mcmdc2
1 of 14
No
No
Mus musculus (laboratory mouse) (8)
Mmus\Mcm5
14 of 14
Yes
Yes
Mmus\Mcm2
3 of 14
No
No
Mmus\Mcm4
3 of 14
No
No
Mmus\Mcm6
3 of 14
No
No
Mmus\Mcm7
3 of 14
No
No
Mmus\Mcm8
3 of 14
No
No
Mmus\Mcm3
2 of 14
No
No
Mmus\Mcm9
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (12)
Xtro\mcm5
11 of 13
Yes
Yes
Xtro\mcm2
2 of 13
No
No
Xtro\mcm4
2 of 13
No
No
Xtro\mcm6.2
2 of 13
No
No
Xtro\mcm6
2 of 13
No
No
Xtro\mcm7
2 of 13
No
No
Xtro\mcm8
2 of 13
No
No
Xtro\mcm9
2 of 13
No
Yes
Xtro\zmcm3
2 of 13
No
No
Xtro\hdlbp
1 of 13
No
No
Xtro\mcm3
1 of 13
No
Yes
Xtro\sumf2
1 of 13
No
No
Danio rerio (Zebrafish) (10)
Drer\mcm5
14 of 14
Yes
Yes
Drer\mcm3
3 of 14
No
No
Drer\mcm3l
3 of 14
No
No
Drer\mcm4
3 of 14
No
No
Drer\mcm6
3 of 14
No
No
Drer\mcm6l
3 of 14
No
No
Drer\mcm8
3 of 14
No
No
Drer\mcm2
2 of 14
No
No
Drer\mcm7
2 of 14
No
No
Drer\mcm9
2 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\mcm-5
14 of 14
Yes
Yes
Cele\mcm-3
3 of 14
No
No
Cele\mcm-4
3 of 14
No
No
Cele\mcm-7
3 of 14
No
No
Cele\mcm-2
2 of 14
No
No
Cele\mcm-6
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (10)
Agam\AgaP_AGAP004408
12 of 12
Yes
Yes
Agam\AgaP_AGAP001135
3 of 12
No
No
Agam\AgaP_AGAP001901
3 of 12
No
Yes
Agam\AgaP_AGAP002580
3 of 12
No
No
Agam\AgaP_AGAP002581
3 of 12
No
No
Agam\AgaP_AGAP004275
3 of 12
No
No
Agam\AgaP_AGAP004956
3 of 12
No
No
Agam\AgaP_AGAP005800
3 of 12
No
No
Agam\MCM6_ANOGA
3 of 12
No
No
Agam\AgaP_AGAP012691
2 of 12
No
No
Arabidopsis thaliana (thale-cress) (8)
Atha\MCM5
13 of 13
Yes
Yes
Atha\MCM2
3 of 13
No
No
Atha\MCM3
3 of 13
No
No
Atha\MCM4
3 of 13
No
No
Atha\MCM6
3 of 13
No
No
Atha\MCM8
3 of 13
No
No
Atha\MCM9
3 of 13
No
Yes
Atha\PRL
3 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\MCM5
13 of 13
Yes
Yes
Scer\MCM2
2 of 13
No
No
Scer\MCM3
2 of 13
No
No
Scer\MCM4
2 of 13
No
No
Scer\MCM6
2 of 13
No
No
Scer\MCM7
2 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (6)
Spom\mcm5
12 of 12
Yes
Yes
Spom\mcm2
2 of 12
No
No
Spom\mcm3
2 of 12
No
No
Spom\mcm4
2 of 12
No
No
Spom\mcm6
2 of 12
No
No
Spom\mcm7
2 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\yifB
1 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Mcm5. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (7)
dpa
6 of 13
Mcm2
6 of 13
Mcm3
6 of 13
Mcm6
6 of 13
Mcm7
6 of 13
rec
6 of 13
mei-218
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MCM5; minichromosome maintenance complex component 5
    14 of 14
    602696
     
    MCM2; minichromosome maintenance complex component 2
    3 of 14
    116945
    MCM4; minichromosome maintenance complex component 4
    3 of 14
    602638
    MCM6; minichromosome maintenance complex component 6
    3 of 14
    601806
    MCM7; minichromosome maintenance complex component 7
    3 of 14
    600592
        MCM8; minichromosome maintenance 8 homologous recombination repair factor
        3 of 14
        608187
        MCM9; minichromosome maintenance 9 homologous recombination repair factor
        3 of 14
        610098
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Mcm5 - CG3430
        experimental knowledge based
        (Guruharsha et al., 2011)
        Mcm5 - Cdc45
        cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, molecular weight estimation by silver staining, anti tag western blot, mass spectrometry studies of complexes
        (Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006, Loebel et al., 2000)
        Mcm5 - Mcm2
        anti bait coimmunoprecipitation, western blot, electron microscopy, experimental knowledge based, cosedimentation
        (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011, Moyer et al., 2006, Loebel et al., 2000)
        Mcm5 - Mcm3
        pull down, western blot, anti tag coimmunoprecipitation, experimental knowledge based, cosedimentation, electron microscopy, anti bait coimmunoprecipitation
        (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011, Loebel et al., 2000)
        Mcm5 - Mcm6
        cosedimentation, electron microscopy, experimental knowledge based
        (Costa et al., 2011, Guruharsha et al., 2011)
        Mcm5 - Mcm7
        cosedimentation, electron microscopy, experimental knowledge based
        (Costa et al., 2011, Guruharsha et al., 2011)
        Mcm5 - Psf1
        anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy
        (Costa et al., 2011, Moyer et al., 2006)
        Mcm5 - Psf2
        anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy
        (Costa et al., 2011, Moyer et al., 2006)
        Mcm5 - Psf3
        anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy
        (Costa et al., 2011, Moyer et al., 2006)
        Mcm5 - Sld5
        anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy
        (Costa et al., 2011, Moyer et al., 2006)
        Mcm5 - dpa
        anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy, experimental knowledge based
        (Costa et al., 2011, Guruharsha et al., 2011, Moyer et al., 2006, Loebel et al., 2000)
        Summary of Genetic Interactions
        Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).
        (UniProt, Q9VGW6 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-50
        Cytogenetic map
        86C6-86C6
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        86C6-86C6
        Limits computationally determined from genome sequence between P{PZ}hth05745 and P{EP}EP3340EP3340
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        86C1-86C15
        (Su, 1996.12.31)
        Experimentally Determined Recombination Data
        Location

        3-50

        (FlyBase, 2016)

        3-47.1

        (Comeron et al., 2012)

        3-

        (Su, 1996.12.31)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (9)
        Genomic Clones (19)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (81)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Su et al., 1997)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
          Other Comments

          Mcm5 is essential for endoreplication.

          (Park and Asano, 2008)

          Mcm5 has a role in mitotic DNA replication. Mcm5 also has a function in the maturation of double strand breaks into crossovers during meiosis.

          (Lake et al., 2007)

          The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

          (Moyer et al., 2006)

          Mcm5 is a member of a gene family with extensive sequence identity to the MCM (minichromosome maintenance) genes of S.cerevisiae.

          (Su et al., 1997)

          Physical interactions exist between three MCM proteins, dpa, Mcm2 and Mcm5. The three proteins reside predominantly in stable multi-protein complexes of about 600kD. Coimmunoprecipitation experiments indicate there are at least two distinct 600kD complexes, one of which includes Mcm5 and the other dpa.

          (Su et al., 1996)
          Relationship to Other Genes
          Source for database merge of
          Additional comments
          Nomenclature History
          Source for database identify of
          Nomenclature comments
          Etymology
          Synonyms and Secondary IDs (14)
          Reported As
          Symbol Synonym
          CG4082
          (Çiçek et al., 2016, Hosono et al., 2015, Schertel et al., 2013, Christis and Munro, 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Sandmann et al., 2006, Jin et al., 2001)
          DmCDC46
          DmCDC465
          (Su et al., 1996)
          DmMCM5
          (Kearsey and Labib, 1998, Ohno et al., 1998, Su and O'Farrell, 1998, Su and O'Farrell, 1997, Su et al., 1997, Su, 1996.12.31)
          DmMcm5
          (Loebel et al., 2000)
          DmeMCM5
          (Blanton et al., 2005)
          MCM5
          (Balasov et al., 2020, Bhargava et al., 2020, Hua and Orr-Weaver, 2017, Balasov et al., 2015, Bleichert et al., 2015, Xie et al., 2015, Das et al., 2013, Chen et al., 2011, Crevel et al., 2011, Gallone et al., 2011, Ilves et al., 2010, Morris et al., 2008, Crevel et al., 2007, Hong et al., 2007, Hediger and Gasser, 2006, Claycomb et al., 2002, Crevel et al., 2001, Feger, 1999)
          McM5
          (De Lucia et al., 2005)
          Mcm5
          (Graziadio et al., 2025, Curnutte et al., 2023, Rubio-Ferrera et al., 2022, Ferguson et al., 2021, Ota et al., 2021, Sureka and Mishra, 2021, Yang, 2021, Kose et al., 2020, Williams et al., 2020, Burnham et al., 2019, Parker et al., 2019, Vorobyeva and Mazina, 2019, Kohzaki, 2018, Sureka et al., 2018, Völker-Albert et al., 2016, Wei et al., 2015, Costa et al., 2014, Liang et al., 2014, Luck et al., 2014, Yan et al., 2014, Kwon et al., 2013, Schertel et al., 2013, Érdi et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Ji et al., 2012, Longworth et al., 2012, Costa et al., 2011, Friedman et al., 2011, Baig et al., 2010, Maqbool et al., 2010, Wasbrough et al., 2010, Casper and Van Doren, 2009, Park and Asano, 2008, Foglietti et al., 2006, Moyer et al., 2006, Wawersik et al., 2005, Stanyon et al., 2004, Cayirlioglu et al., 2003)
          PCR4
          (Su et al., 1997)
          mcm5
          (Hughes et al., 2018, Park and Asano, 2008, Tanneti et al., 2008, Lake et al., 2007, Page et al., 2007, Arbouzova and Zeidler, 2006, Lake and Hawley, 2006, Nielsen et al., 2006, Jasper et al., 2002)
          Name Synonyms
          Minichromosome maintenance 5
          (Érdi et al., 2012, Baig et al., 2010, Casper and Van Doren, 2006)
          mini-chromosome maintenance 5
          (Casper and Van Doren, 2009)
          minichromosome maintenance 5
          (Page et al., 2007)
          Secondary FlyBase IDs
            Datasets (1)
            Study focus (1)
            Experimental Role
            Project
            Project Type
            Title
            • bait_protein
            modENCODE_replication_factor_ChIP
            Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 51 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            PDB - An information portal to biological macromolecular structures
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (132)