FlyBase Allele Report: Dmel\E2f1[hs.PD]

General Information

Symbol

Dmel\E2f1^hs.PD

Species

D. melanogaster

Name

heat shock construct of Duronio

FlyBase ID

FBal0044772

Feature type

allele

Associated gene

Dmel\E2f1

Associated Insertion(s)

Carried in Construct

P{hs-dE2F.D}

Also Known As

HS-E2F, hsp70-dE2F1

Key Links

Transgenic product class

cDNA

(Duronio et al., 1995)

wild_type

(Duronio et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Duronio et al., 1995)

wild_type

(Duronio et al., 1995)

Mutagen

in vitro construct

(Duronio et al., 1995)

Progenitor genotype

Carried in construct

P{hs-dE2F.D}

Cytology

Description

Construct: 4.4kb E2f cDNA is expressed from a heat shock promoter.

(Duronio et al., 1995)

Allele components

Component

Use(s)

Encoded product / tool

E2f1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with E2f1⁹¹

(Royzman et al., 2002)

Phenotype Manifest In

eye, with E2f1⁹¹

(Royzman et al., 2002)

heterochromatin & nurse cell, with E2f1⁹¹

(Royzman et al., 2002)

Detailed Description

Statement

Reference

E2f⁹¹ animals partially rescued by E2f^hs.PD have rough eyes and are female sterile. Nurse cell heterochromatin is also increased.

(Royzman et al., 2002)

Neuroblasts in trol^sd larval brains show a proliferation defect.

(Caldwell and Datta, 1998)

Mild heat induced expression (25^oC) results in significant rescue of the Dp^a1 mutant phenotype. At 37^oC rescue is dampened. Expression does not rescue the Dp^a2 mutant phenotype.

(Royzman et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Dp^hs.PD, E2f1^hs.PD has abnormal mitotic cell cycle | heat sensitive phenotype, suppressible | partially by CycE^AR95

(Duronio et al., 1996)

Suppressor of

Statement

Reference

E2f1^hs.PD/Dp^hs.PD is a suppressor of abnormal size | larval stage phenotype of Myb^Δ.UAS, Scer\GAL4^sd-SG29.1

(Fitzpatrick et al., 2002)

E2f1^hs.PD is a suppressor of abnormal developmental rate | larval stage phenotype of RpL30^k13307

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased occurrence of cell division | larval stage phenotype of RpL30^k13307

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased body size | larval stage phenotype of AspRS^k05411

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased occurrence of cell division | larval stage phenotype of AspRS^k05411

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased occurrence of cell division | larval stage phenotype of blw^k04409

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased cell number | somatic clone | cell autonomous | larval stage phenotype of blw^k04409

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased cell number | somatic clone | cell autonomous | larval stage phenotype of milou^k13910

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased occurrence of cell division | larval stage phenotype of milou^k13910

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of abnormal developmental rate | larval stage phenotype of RpL30^k00308

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased occurrence of cell division | larval stage phenotype of RpL30^k00308

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of abnormal developmental rate | larval stage phenotype of RpL30^k13302

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of decreased occurrence of cell division | larval stage phenotype of RpL30^k13302

(Galloni and Edgar, 1999)

Other

Statement

Reference

Dp^hs.PD, E2f1^hs.PD has abnormal mitotic cell cycle | heat sensitive phenotype

(Duronio et al., 1996)

Phenotype Manifest In

Suppressed by

Statement

Reference

Dp^hs.PD, E2f1^hs.PD has epidermis phenotype, suppressible by CycE^AR95

(Duronio et al., 1996)

NOT suppressed by

Statement

Reference

Dp^hs.PD, E2f1^hs.PD has embryonic/larval midgut | embryonic stage phenotype, non-suppressible by CycE^AR95

(Duronio et al., 1996)

Dp^hs.PD, E2f1^hs.PD has embryonic/larval salivary gland | embryonic stage phenotype, non-suppressible by CycE^AR95

(Duronio et al., 1996)

Enhancer of

Statement

Reference

E2f1^hs.PD is an enhancer of wing | heat sensitive phenotype of Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096

(Heriche et al., 2003)

Suppressor of

Statement

Reference

E2f1^hs.PD/Dp^hs.PD is a suppressor of mitotic cell cycle phenotype of Myb^Δ.UAS, Scer\GAL4^sd-SG29.1

(Fitzpatrick et al., 2002)

E2f1^hs.PD/Dp^hs.PD is a suppressor of embryonic/larval salivary gland phenotype of Myb^Δ.UAS, Scer\GAL4^sd-SG29.1

(Fitzpatrick et al., 2002)

E2f1^hs.PD is a suppressor of phenotype of blw^k04409

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of phenotype of milou^k13910

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of phenotype of AspRS^unspecified

(Galloni and Edgar, 1999)

E2f1^hs.PD is a suppressor of phenotype of RpL30^unspecified

(Galloni and Edgar, 1999)

Other

Statement

Reference

Dp^hs.PD, E2f1^hs.PD has embryonic/larval salivary gland | embryonic stage phenotype

(Duronio et al., 1996)

Dp^hs.PD, E2f1^hs.PD has epidermis phenotype

(Duronio et al., 1996)

Dp^hs.PD, E2f1^hs.PD has embryonic/larval midgut | embryonic stage phenotype

(Duronio et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of both E2f^hs.PD and Dp^hs.PD (using heat shock) in larvae expressing Myb^Δ.Scer\UAS under the control of Scer\GAL4^sd-SG29.1 results in partial rescue of the salivary glands, both with respect to the overall size of the glands and the size of the individual nuclei, and BrdU incorporation can be detected in a number of the salivary gland nuclei with a couple of hours of heat treatment.

(Fitzpatrick et al., 2002)

Suppresses the DNA replication defects of milou^k13910, eIF-4a¹⁰⁰⁶, eIF-4a^162-3, eIF-4a¹⁰⁶⁹, eIF-4a¹⁰¹³, bonsai^k08322, blw^k04409 and alleles of Aats-asp and RpL30.

(Galloni and Edgar, 1999)

Co-expression of both E2f1^hs.PD and Dp^hs.PD in starved larvae using heat shock is sufficient to drive most endoreplicating cells into S phase, as assayed by BrdU incorporation. Neuroblasts do not incorporate BrdU in these larvae.

(Britton and Edgar, 1998)

Expression of both Dp^hs.PD and E2f^hs.PD in trol^sd larvae using heat shock is able to rescue the proliferation defect seen in trol^sd larval brains.

(Caldwell and Datta, 1998)

Heat induced expression of E2f1^hs.PD at 25[o]C fails to provide rescue of the mutant phenotype.

(Royzman et al., 1997)

Simultaneous over-expression of E2f^hs.PD and Dp^hs.PD induces DNA replication in epidermal cells of stage 13 or 14 embryos (when these cells are normally arrested in cycle 17). Midgut and salivary gland endocycles are also affected. Over-expression of E2f^hs.PD or Dp^hs.PD alone has no effect. In a CycE^AR95 mutant background, no DNA replication is induced in stage 13 or 14 embryos by simultaneous over-expression of E2f^hs.PD and Dp^hs.PD. In a heterozygous CycE^AR95 mutant background simultaneous over-expression of E2f^hs.PD and Dp^hs.PD causes induction of S phase in the salivary gland and midgut, but not the epidermis.

(Duronio et al., 1996)

Xenogenetic Interactions

Statement

Reference

Increased levels of E2f1 through expression of E2f1^hs.PD enhances the small wing phenotype in Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 over-expression mutants. This enhancement is stronger and completely penetrant when exposed to heat shock treatment every 24 hours during third instar and early pupal development.

(Heriche et al., 2003)

Complementation and Rescue Data

Partially rescues

E2f1^hs.PD partially rescues E2f1⁹¹

(Royzman et al., 2002)

Comments

E2f^hs.PD Partially rescues the lethality seen in E2f⁹¹ mutants.

(Royzman et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E2f1^hs.PD

E2f^hs.PD

Name Synonyms

heat shock construct of Duronio

(Duronio et al., 1995)

Secondary FlyBase IDs

References (14)

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