FlyBase Allele Report: Dmel\Kdm5[k06801]

General Information

Symbol

Dmel\Kdm5^k06801

Species

D. melanogaster

Name

FlyBase ID

FBal0064675

Feature type

allele

Associated gene

Dmel\Kdm5

Associated Insertion(s)

P{lacW}Kdm5^k06801

Carried in Construct

Also Known As

lid^k06801, lid², kdm5^K6801, l(2)k06801, lid^k6801

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Kdm5^k06801

Cytology

Description

P{lacW} insertion in the first intron.

(Gildea et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autism spectrum disorder

CEA with Kdm5¹⁰⁴²⁴

(Chen et al., 2019)

model of alcohol use disorder

CEA

(Pinzón et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromosome, centromeric region | adult stage (with Kdm5¹⁰⁴²⁴)

(Zhaunova et al., 2016)

karyosome | adult stage (with Kdm5¹⁰⁴²⁴)

(Zhaunova et al., 2016)

larval somatic muscle cell

(Dobi et al., 2014)

larval somatic muscle cell (with Df(2L)BSC184)

(Dobi et al., 2014)

male germline stem cell | adult stage | somatic clone

(Tarayrah et al., 2015)

mitochondrion | adult stage (with Kdm5¹⁰⁴²⁴)

(Liu and Secombe, 2015)

oocyte (with Kdm5¹⁰⁴²⁴), with Kdm5^JmjC.Tag:HA

(Navarro-Costa et al., 2016)

synaptonemal complex | adult stage (with Kdm5¹⁰⁴²⁴)

(Zhaunova et al., 2016)

Detailed Description

Statement

Reference

lid^k06801 mutants are near lethal and the few escapers are morphological normal but short-lived.

(Drelon et al., 2018)

lid¹⁰⁴²⁴/lid^k06801;lid^{JmjC.T:Ivir\HA1} 'rescue' flies display premature transcriptional reactivation as well as precocious karyosome remodeling (the oocytes display early increase in chromatin openness) in prophase I-arrested oocytes and the eggs show impaired entry into embryogenesis after fertilization resulting in strongly reduced female fertility.

(Navarro-Costa et al., 2016)

lid^k06801/lid¹⁰⁴²⁴ mutant female germlines exhibit abnormal morphology of the karyosome, defective clustering of centromeres, and a failure to maintain the synaptonemal complex.

(Zhaunova et al., 2016)

lid^k06801/lid¹⁰⁴²⁴ mutant flies have irregularly shaped mitochondria that are larger than those of wild-type flies and the flies display reduced climbing ability.

lid^k06801 homozygotes survive at less than 5% rate, whereas about 50% of lid^k06801/lid¹⁰⁴²⁴ mutants survive.

lid^k06801 mutant flies rescued with any of the following: lid^gWT, lid^gJmjC.mut or lid^W1771A do not show any mitochondrial abnormalities in the thoracic muscles.

(Liu and Secombe, 2015)

The percentage of testes with at least one lid^k06801/lid^k06801 germline stem cell clone declines over time compared to wild type.

(Tarayrah et al., 2015)

Homozygotes reach pupal development at 71% of expected frequency, but only 0.5% of the expected number of homozygotes survive to adulthood.

(Li et al., 2010)

lid^k06801/lid¹⁰⁴²⁴ animals eclose at approximately half the expected frequency.

(Secombe et al., 2007)

Some lid^k06801/lid¹⁰⁴²⁴ flies appear to die before hatching, although no obvious defects in the larval cuticle are seen.

(Gildea et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Kdm5^k06801 has lethal | recessive phenotype, enhanceable by Kdm2^DG12810/Kdm2^DG12810

(Li et al., 2010)

Kdm5^k06801/Kdm5¹⁰⁴²⁴ has partially lethal phenotype, enhanceable by Myc^P0

(Secombe et al., 2007)

Enhancer of

Statement

Reference

Kdm5^k06801 is an enhancer of partially lethal - majority die phenotype of Kdm2^DG12810

(Li et al., 2010)

Kdm5^k06801/lid[+] is an enhancer of visible | dominant phenotype of H^P141

(Moshkin et al., 2009)

Kdm5^k06801/lid[+] is an enhancer of visible | dominant | homeotic phenotype of brm[+]/brm², trx^E2

(Gildea et al., 2000)

Kdm5^k06801/lid[+] is an enhancer of visible | dominant | homeotic phenotype of ash1¹⁷, trx^B11/trx[+]

(Gildea et al., 2000)

NOT Enhancer of

Statement

Reference

Kdm5^k06801 is a non-enhancer of visible phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Suppressor of

Statement

Reference

Kdm5^k06801/lid[+] is a suppressor of visible | dominant phenotype of Snr1^E1

(Curtis et al., 2011)

Kdm5^k06801/lid[+] is a suppressor of visible phenotype of Scer\GAL4^ey.PH, asf1^UAS.cMa

(Moshkin et al., 2009)

Kdm5^k06801/lid[+] is a suppressor of visible | dominant phenotype of N^55e11

(Moshkin et al., 2009)

Kdm5^k06801/lid[+] is a suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

NOT Suppressor of

Statement

Reference

Kdm5^k06801 is a non-suppressor of visible phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Other

Statement

Reference

Kdm2^DG12810, Kdm5^k06801 has lethal | first instar larval stage phenotype

(Li et al., 2010)

Kdm2^DG12810, Kdm5^k06801 has lethal | second instar larval stage phenotype

(Li et al., 2010)

Kdm5^k06801/lid[+], ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5^k06801/lid[+], trx^B11 has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5^k06801/lid[+], brm² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5^k06801, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5^k06801, brm² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Kdm5^k06801, trx^B11 has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

Kdm5^k06801/lid[+] is an enhancer of wing vein L5 phenotype of H^P141

(Moshkin et al., 2009)

Kdm5^k06801 is an enhancer of eye phenotype of CycE^JP

(Brumby et al., 2002)

Kdm5^k06801/lid[+] is an enhancer of adult metathoracic segment phenotype of brm[+]/brm², trx^E2

(Gildea et al., 2000)

Kdm5^k06801/lid[+] is an enhancer of adult metathoracic segment phenotype of ash1¹⁷, trx^B11/trx[+]

(Gildea et al., 2000)

NOT Enhancer of

Statement

Reference

Kdm5^k06801 is a non-enhancer of eye phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Suppressor of

Statement

Reference

Kdm5^k06801/lid[+] is a suppressor of wing vein | ectopic phenotype of Snr1^E1

(Curtis et al., 2011)

Kdm5^k06801/lid[+] is a suppressor of eye phenotype of Dref^UAS.cSa

(Ida et al., 2009)

Kdm5^k06801/lid[+] is a suppressor of eye phenotype of Scer\GAL4^ey.PH, asf1^UAS.cMa

(Moshkin et al., 2009)

Kdm5^k06801/lid[+] is a suppressor of wing vein phenotype of N^55e11

(Moshkin et al., 2009)

Kdm5^k06801/lid[+] is a suppressor of eye phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

NOT Suppressor of

Statement

Reference

Kdm5^k06801 is a non-suppressor of eye phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Other

Statement

Reference

Kdm5^k06801/lid[+], ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5^k06801/lid[+], trx^B11 has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5^k06801/lid[+], brm² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5^k06801, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5^k06801, brm² has adult metathoracic segment phenotype

(Gildea et al., 2000)

Kdm5^k06801, trx^B11 has adult metathoracic segment phenotype

(Gildea et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

lid^k06801 Kdm2^DG12810 double homozygotes show enhanced lethality compared to either single mutant, dying during the first and second larval instar stages.

(Li et al., 2010)

The small, rough eye phenotype caused by expression of asf1^Scer\UAS.cMa under the control of Scer\GAL4^ey.PH is significantly suppressed by lid^k06801/+.

The thickening of the wing veins that is seen in N^55e11/+ flies is suppressed by lid^k06801/+.

The truncation of wing vein L5 that is seen in H^P141/+ heterozygotes is enhanced by lid^k06801/+.

(Moshkin et al., 2009)

The dm^P0 ; lid^k06801/lid¹⁰⁴²⁴ combination results in almost complete lethality.

(Secombe et al., 2007)

Double heterozygotes with ash1²², brm² or trx^B11 show T3 to T2 transformations. The ectopic sex comb teeth seen on the second leg of Df(3L)Pc heterozygotes are partially suppressed by one copy of lid^k06801.

(Gildea et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Kdm5¹⁰⁴²⁴

(Gildea et al., 2000)

Kdm5¹⁰⁴²⁴

(BDGP Project Members, 1994-1999)

Rescued by

Kdm5^k06801/Kdm5¹⁰⁴²⁴ is rescued by Kdm5^Tag:HA

(Navarro-Costa et al., 2016)

Kdm5^k06801/Kdm5¹⁰⁴²⁴ is rescued by Kdm5^gJmjC.mut

(Zhaunova et al., 2016)

Kdm5^k06801/Kdm5¹⁰⁴²⁴ is rescued by Kdm5^gWT

(Zhaunova et al., 2016)

Kdm5^k06801 is rescued by Kdm5^gWT

(Li et al., 2010)

Comments

The premature transcriptional reactivation as well as precocious karyosome remodeling (the oocytes display early increase in chromatin openness) in prophase I-arrested lid¹⁰⁴²⁴/lid^k06801 transheterozygous oocytes is rescued by combination with lid^T:Ivir\HA1.

(Navarro-Costa et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10403

y¹ w^67c23; P{lacW}Kdm5^k06801/CyO

Kyoto

111088

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Kdm5^k06801 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: eIF-4a⁰²⁴³⁹.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

Kdm5^k06801

(Herz and Bergmann, 2024)

Lid^K0681

(Lee and Spradling, 2014)

dkdm5^K06801

(Navarro-Costa et al., 2016)

kdm5^K06801

(Drelon et al., 2018, Liu et al., 2014)

kdm5^K6801

(Liu and Secombe, 2015, Liu et al., 2014)

kdm5^k06801

(Chen et al., 2019)

kdm5^k6801

(Chen et al., 2019)

l(2)10424^k06801

(BDGP Project Members, 1994-1999)

l(2)k06801

(Gildea et al., 2000, Spradling et al., 1999)

lid¹⁰⁴⁰³

(Lloret-Llinares et al., 2008)

lid²

(Curtis et al., 2011, Brumby et al., 2002, Gildea et al., 2000)

lid^k06801

(Shalaby et al., 2018, Shalaby et al., 2017, Zhaunova et al., 2016, Tarayrah et al., 2015, Dobi et al., 2014, Ji et al., 2012, Di Stefano et al., 2011, Ida et al., 2009, Moshkin et al., 2009)

lid^k6801

(Li et al., 2010, Secombe et al., 2007)

lid^k

(Pinzón et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (26)

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