nurse cell & actin filament
SxlM4, dmP0/dmP0 females are fully viable. While a SxlM4/+ background rescues the female-specific lethality of dmP0, it does not rescue the dmP0 bristle or female sterility phenotypes. No SxlM4, dmP0/dmP0 males are recovered from the cross.
One copy of dmP0 suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. The late larval lethality is also partially suppressed.
The reduced size of the posterior compartment of the wing that is seen in animals expressing crolScer\UAS.cMa under the control of Scer\GAL4en-e16E is suppressed if they are also heterozygous for dmP0.
Eyes consisting of dmP0 homozygous clones (induced using the ey-FLP method) that are also heterozygous for Brfc07161 show a significant reduction in eye size compared to controls and clear eye defects (rough eye) are seen in 17% of the animals.
dmP0 males carrying one copy of pont5.1 suffer a drop in viability from 54% to 12%, along with the surviving flies taking 2.4 days longer for their development and, despite the extended period, ultimately eclose with a significantly lower weight. dmP0 males, heterozygous for pont5.1 occasionally exhibit notches in the posterior wing margin. There is a significant decrease in the total wing area of dmP0 males heterozygous for pont5.1, compared to the control.A large part of this size difference can be attributed to a decrease in cell size. Approximately 90% of dmP0 males, heterozygous for pont5.1 have small, irregularly shaped, and slightly rough eyes. Penetrance and expressivity of the phenotype are variable, but the anterior and ventral portions of the eye are always most defective. The significant reduction of ommatidial size in dmP0 males is exacerbated by heterozygosity of pont5.1. In addition, these flies contain a reduced number of ommatidia. Comparison of control, dmP0 males, and dmP0 males in a pont5.1 heterozygous background in third instar larval eye imaginal discs does not reveal any obvious defects in any of the three genotypes with respect to overall shape and the pattern of differentiating ommatidia, although eye discs of dmP0 males in a pont5.1 heterozygous background are significantly smaller than those of the controls or dmP0 males. The ectopic expression of reptScer\UAS.T:BPV-1\AU1, under the regulation of Scer\GAL4tub in dmP0 somatic clones enhances the penetrance and expressivity of their eye defects. Even when over-expressed in an dmP0 eye somatic clones or a wild-type background. The ectopic expression of pontScer\UAS.T:Hsap\MYC, under the regulation of Scer\GAL4tub in dmP0 clones suppresses the dmP0 eye defects of ommatidia decreased size and number.