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General Information
Symbol
Dmel\MycP0
Species
D. melanogaster
Name
FlyBase ID
FBal0100372
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dmP0, dmycP0
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of P{P-Sal} 99 nucleotides upstream of the longest isolated dm clone.

Insertion components
P{P-Sal}MycP0
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

MycP0 ovaries show decreased mtDNA replication in region 2B and 3 cysts, as compared to controls.

MycP0/+ third instar larvae are significantly smaller than the wild-type controls.

Approximately 46% of dmP0 homozygous females survive to adulthood, compared with 73% of sibling dm1/Y males.

Irradiation of mutant eye discs with 5 or 10 Gy results in fewer apoptotic cells than irradiation of wild-type disc.

The overall duration of development is extended by one day in dmP0/Y flies compared to wild type.

Eyes consisting of dmP0 homozygous clones (induced using the ey-FLP method) are phenotypically wild type.

Homozygous pupae are more sensitive to X rays than wild type or heterozygous sibling controls.

dmP0 males are characterised by a slightly delayed development, thin bristles, and a weak reduction in body size and viability (to 54%). dmP0 males exhibit a weak eye phenotype with small, irregularly shaped, slightly rough eyes. dmP0 males exhibit a significant reduction in ommatidial size. All dmP0 male mutant eye disc clones are significantly smaller than control discs.

dmP0 homozygous mutant first instar larvae are the same size as wild-type controls. dmP0 homozygous mutant cells generated by Flp/FRT recombination in the wing disc exhibit a decrease in nucleolar size compared with surrounding heterozygote cells.

When homozygous somatic clones are made in the wing disc, they exhibit signs of apoptosis and the clones themselves appear to be extruded basally on both sides of the lumen.

Oogenesis in dmP0 homozygous females is arrested between stages 10 and 11: cytoplasmic actin bundles failed to develop around stage 11 nurse cell nuclei, and nurse cell death fails to occur.

More severe phenotype than dm1. Thin and shortened bristles. Small body size. Size of wing cells, wing width and wing length are reduced. Developmental rate is reduced and flies occasionally have small, roughened eyes. Dissociated wing disc cells are reduced in size compared to wild type. Twin spot analysis of mutant clones shows that dm mutant cells grow very poorly, particularly in the posterior compartment of the wing disc.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

MycP0 has abnormal developmental rate phenotype, enhanceable by pont[+]/pont5.1

Suppressed by
Statement
Reference

MycP0 has lethal | female limited phenotype, suppressible | partially by scsisB-1

MycP0 has lethal | female limited phenotype, suppressible | partially by run3

MycP0 has decreased cell death | recessive phenotype, suppressible by pUf68[+]/hfpEP3058/Scer\GAL4[-]

MycP0 has female sterile | recessive phenotype, suppressible by pUf68[+]/hfpEP3058/Scer\GAL4[-]

NOT suppressed by
Statement
Reference

MycP0 has abnormal size phenotype, non-suppressible by agounspecified

Enhancer of
Statement
Reference
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

MycP0 has eye phenotype, enhanceable by pont[+]/pont5.1

MycP0 has ommatidium phenotype, enhanceable by pont[+]/pont5.1

Suppressed by
Statement
Reference

MycP0 has nurse cell & actin filament phenotype, suppressible by pUf68[+]/hfpEP3058/Scer\GAL4[-]

MycP0 has nurse cell phenotype, suppressible by pUf68[+]/hfpEP3058/Scer\GAL4[-]

MycP0 has oocyte | oogenesis stage S11 phenotype, suppressible by pUf68[+]/hfpEP3058/Scer\GAL4[-]

Enhancer of
Statement
Reference

dm[+]/MycP0 is an enhancer of eye phenotype of Hsap\CDKN1AGMR.PH

dm[+]/MycP0 is an enhancer of ommatidium phenotype of Hsap\CDKN1AGMR.PH

Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The large body size characteristic for hfpEP3058/hfpEP3058 third instar larvae is rescued by combination with MycP0 in heterozygous state.

The small body size characteristic for MycP0/+ third instar larvae can be suppressed by combination with haync2rv1 in heterozygous state.

The severe body overgrowth seen in hfpEP3058/hfpEP3058;haync2rv1/+ third instar larvae can be rescued by combination with MycP0 in heterozygous state.

Approximately 88% of sisAunspecified dmP0 trans-heterozygous female offspring from dmP0 fathers mated to sisAunspecified mothers survive.

Approximately 90% of scsisB-1 dmP0 trans-heterozygous female offspring from dmP0 fathers mated to scsisB-1 mothers survive.

Approximately 79% of run3 dmP0 trans-heterozygous female offspring from dmP0 fathers mated to run3 mothers survive.

Females trans-heterozygous for SxlfP7B0 and dmP0 are approximately two-thirds as viable as their dmP0/+ siblings.

SxlM4, dmP0/dmP0 females are fully viable. While a SxlM4/+ background rescues the female-specific lethality of dmP0, it does not rescue the dmP0 bristle or female sterility phenotypes. No SxlM4, dmP0/dmP0 males are recovered from the cross.

Clonal expression of either HcfdsRNA.IR-2.Scer\UAS or HcfdsRNA.IR-3.Scer\UAS under the control of Scer\GAL4αTub84B.PBb in a dmP0 background results in flies with smaller ommatidia than normal.

One copy of dmP0 suppresses the glial neoplasia seen in third instar larvae when btl::EgfrScer\UAS.T:λ\cI-DD and Pi3K92EScer\UAS.T:Hsap\MYC,T:Hsap\CAAX are co-expressed under the control of Scer\GAL4repo.PU. The late larval lethality is also partially suppressed.

The reduced size of the posterior compartment of the wing that is seen in animals expressing crolScer\UAS.cMa under the control of Scer\GAL4en-e16E is suppressed if they are also heterozygous for dmP0.

dmP0/Y ; Max1/+ flies take slightly longer for their development than controls.

dmP0/Y ; flies expressing MaxdsRNA.Scer\UAS.IR under the control of Scer\GAL4da.G32 show a strong reduction in viability compared to controls.

Eyes consisting of dmP0 homozygous clones (induced using the ey-FLP method) that are also heterozygous for Brfc07161 show a significant reduction in eye size compared to controls and clear eye defects (rough eye) are seen in 17% of the animals.

The dmP0 ; lidk06801/lid10424 combination results in almost complete lethality.

dmP0 males carrying one copy of pont5.1 suffer a drop in viability from 54% to 12%, along with the surviving flies taking 2.4 days longer for their development and, despite the extended period, ultimately eclose with a significantly lower weight. dmP0 males, heterozygous for pont5.1 occasionally exhibit notches in the posterior wing margin. There is a significant decrease in the total wing area of dmP0 males heterozygous for pont5.1, compared to the control.A large part of this size difference can be attributed to a decrease in cell size. Approximately 90% of dmP0 males, heterozygous for pont5.1 have small, irregularly shaped, and slightly rough eyes. Penetrance and expressivity of the phenotype are variable, but the anterior and ventral portions of the eye are always most defective. The significant reduction of ommatidial size in dmP0 males is exacerbated by heterozygosity of pont5.1. In addition, these flies contain a reduced number of ommatidia. Comparison of control, dmP0 males, and dmP0 males in a pont5.1 heterozygous background in third instar larval eye imaginal discs does not reveal any obvious defects in any of the three genotypes with respect to overall shape and the pattern of differentiating ommatidia, although eye discs of dmP0 males in a pont5.1 heterozygous background are significantly smaller than those of the controls or dmP0 males. The ectopic expression of reptScer\UAS.T:BPV-1\AU1, under the regulation of Scer\GAL4tub in dmP0 somatic clones enhances the penetrance and expressivity of their eye defects. Even when over-expressed in an dmP0 eye somatic clones or a wild-type background. The ectopic expression of pontScer\UAS.T:Hsap\MYC, under the regulation of Scer\GAL4tub in dmP0 clones suppresses the dmP0 eye defects of ommatidia decreased size and number.

ftk07918 clones are induced in a dmP0 background at 72+-2h are severely reduced compared to those induced in wild-type.

The female sterility and oogenesis phenotypes of dmP0 flies are suppressed by pUf68EP3058/+.

Xenogenetic Interactions
Statement
Reference

The small, rough eye phenotype of Hsap\CDKN1AGMR.PH flies is enhanced by dmP0/+.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Alleles can be ranked in a phenotypic series. Going from weakest to strongest phenotype: dm1 < dmP0 < dmP1.

Maintained in compound-X stock; female viability and fertility not specified.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (24)