FlyBase Allele Report: Dmel\hfp[EP3058]

hfp^EP3058 homozygous mutant larvae and pupae are significantly larger than wild-type controls and display an increase in the number mitotic cells and cells in the S-phase of the cell cycle in the third instar larval wing disc.

(Lee et al., 2015)

Pupariation in pUf68^EP3058 homozygous animals is delayed by approximately two days. The resulting wandering third instar larvae and pupae are about 20% larger than wild-type controls. The duration of pupariation is normal but these animals fail to eclose and die as pharate adults that are larger than wild-type, have duplication of the superior scutellar macrochaete and malformation of legs, wings and sex combs. The number of S phase cells and mitotic cells is increased in pUf68^EP3058 mutant wing discs: BrdU incorporation is uniform across the disc and cell cycle arrest is not evident in the zone of non-proliferating cells found at the dorsal-ventral compartment boundary of wild-type late third instar wing-discs. In pUf68^EP3058/pUf68^EP3058 eye discs the S phase band posterior to the morphogenetic furrow is broader than in wild type, but the second mitotic wave does not occur prematurely. Despite the increased proliferation in these discs, they are not overgrown and there is no obvious difference in cell size compared to wild-type. There are, however, around twice as many apoptotic cells in the wing imaginal discs of these mutants than in wild-type. Dividing cells in Cycle 16 pUf68^EP3058/pUf68^EP3058 embryos exhibit abnormal chromosome morphology, including many lagging chromosomes that are often mis-segregated due to closure of the contractile ring prior to sister chromatid separation.

(Quinn et al., 2004)

pUf68^EP3058/Df(3L)Ar14-8 animals are slower to develop than controls and die shortly after pupariation with melanised patches of tissue.

(Van Buskirk and Schupbach, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

hfp^EP3058 has increased body size | third instar larval stage phenotype, enhanceable by hay[+]/hay^nc2rv1

(Lee et al., 2015)

hfp^EP3058 has increased occurrence of cell division | third instar larval stage phenotype, enhanceable by hay[+]/hay^nc2rv1

(Lee et al., 2015)

hfp^EP3058 has increased body size | third instar larval stage phenotype, enhanceable by hay[+]/hay^nc2rv7

(Lee et al., 2015)

hfp^EP3058 has increased occurrence of cell division | third instar larval stage phenotype, enhanceable by hay[+]/hay^nc2rv7

(Lee et al., 2015)

hfp^EP3058 has increased body size | third instar larval stage phenotype, enhanceable by hay[+]/hay^nc2rv3

(Lee et al., 2015)

Suppressed by

Statement

Reference

hay[+]/hay^nc2rv1, hfp^EP3058 has increased body size | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^arm.PU

(Lee et al., 2015)

hfp^EP3058 has increased body size | third instar larval stage phenotype, suppressible by hay[+]/hay^nc2

(Lee et al., 2015)

hfp^EP3058 has increased body size | third instar larval stage phenotype, suppressible by Myc^P0/Myc[+]

(Lee et al., 2015)

hfp^EP3058 has increased occurrence of cell division | third instar larval stage phenotype, suppressible by hay[+]/hay^nc2

(Lee et al., 2015)

hfp^EP3058 has increased occurrence of cell division | third instar larval stage phenotype, suppressible by hay[+]/hay^f00028

(Lee et al., 2015)

hfp^EP3058 has increased occurrence of cell division | third instar larval stage phenotype, suppressible by Myc[+]/Myc^G0354

(Lee et al., 2015)

hay[+]/hay^nc2rv1, hfp^EP3058 has increased body size | third instar larval stage phenotype, suppressible by Myc^P0

(Lee et al., 2015)

hay[+]/hay^nc2rv1, hfp^EP3058 has abnormal cell cycle | third instar larval stage phenotype, suppressible by Myc[+]/Myc^G0354

(Lee et al., 2015)

Enhancer of

Statement

Reference

hfp^EP3058/hfp[+] is an enhancer of abnormal size | adult stage phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Quinn et al., 2004)

Suppressor of

Statement

Reference

hfp^EP3058/hfp[+] is a suppressor of abnormal size | adult stage phenotype of Hsap\CDKN1A^GMR.PH

(Quinn et al., 2004)

pUf68[+]/hfp^EP3058 is a suppressor of decreased cell death | recessive phenotype of Myc^P0

(Quinn et al., 2004)

pUf68[+]/hfp^EP3058 is a suppressor of female sterile | recessive phenotype of Myc^P0

(Quinn et al., 2004)

Other

Statement

Reference

Hsap\CDKN1A^GMR.PH, hfp^EP3058/hfp[+] has increased occurrence of cell division | larval stage phenotype

(Quinn et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

hfp^EP3058 has wing disc | third instar larval stage phenotype, enhanceable by hay[+]/hay^nc2rv1

(Lee et al., 2015)

hfp^EP3058 has wing disc | third instar larval stage phenotype, enhanceable by hay[+]/hay^nc2rv7

(Lee et al., 2015)

Suppressed by

Statement

Reference

hay[+]/hay^nc2rv1, hfp^EP3058 has wing disc | third instar larval stage phenotype, suppressible by Myc^G0354

(Lee et al., 2015)

hfp^EP3058 has wing disc | third instar larval stage phenotype, suppressible by Myc[+]/Myc^G0354

(Lee et al., 2015)

hfp^EP3058 has wing disc | third instar larval stage phenotype, suppressible by hay[+]/hay^f00028

(Lee et al., 2015)

hfp^EP3058 has wing disc | third instar larval stage phenotype, suppressible by hay[+]/hay^nc2

(Lee et al., 2015)

Enhancer of

Statement

Reference

pUf68[+]/hfp^EP3058, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Quinn et al., 2004)

pUf68[+]/hfp^EP3058, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Quinn et al., 2004)

Suppressor of

Statement

Reference

pUf68[+]/hfp^EP3058, Scer\GAL4^GMR.PF is a suppressor of eye disc phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Quinn et al., 2004)

hfp^EP3058/hfp[+] is a suppressor of ommatidium phenotype of Hsap\CDKN1A^GMR.PH

(Quinn et al., 2004)

hfp^EP3058/hfp[+] is a suppressor of eye phenotype of Hsap\CDKN1A^GMR.PH

(Quinn et al., 2004)

pUf68[+]/hfp^EP3058 is a suppressor of nurse cell & actin filament phenotype of Myc^P0

(Quinn et al., 2004)

pUf68[+]/hfp^EP3058 is a suppressor of nurse cell phenotype of Myc^P0

(Quinn et al., 2004)

pUf68[+]/hfp^EP3058 is a suppressor of oocyte | oogenesis stage S11 phenotype of Myc^P0

(Quinn et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The large body size as well as the elevated proliferation rate in the wing disc characteristic for the hfp^EP3058/hfp^EP3058 third instar larvae is further increased by combination with hay^nc2rv1 in heterozygous state. The increased body size of hfp^EP3058/hfp^EP3058;hay^nc2rv1/+ larvae can be partially suppressed by driving driving hfp expression from the UAS included in the hfp^EP3058 P-element insertion allele under the control of the Scer\GAL4^arm.PU driver.

The large body size as well as the elevated proliferation rate (number of mitotic and S-phase cells) in the wing disc characteristic for the hfp^EP3058/hfp^EP3058 third instar larvae can be restored by combination with hay^nc2 in heterozygous state.

The large body size as well as the higher number of mitotic cells in the wing disc characteristic for the hfp^EP3058/hfp^EP3058 third instar larvae is further increased by combination with hay^nc2rv7 in heterozygous state.

The large body size characteristic for the hfp^EP3058/hfp^EP3058 third instar larvae is further increased by combination with hay^nc2rv1 in heterozygous state.

The increased number of mitotic cells in the wing disc characteristic for the hfp^EP3058/hfp^EP3058 third instar larvae can be rescued by combination with hay^f00028 in heterozygous state.

The large body size characteristic for hfp^EP3058/hfp^EP3058 third instar larvae is rescued by combination with Myc^P0 in heterozygous state.

The severe body overgrowth seen in hfp^EP3058/hfp^EP3058;hay^nc2rv1/+ third instar larvae can be rescued by combination with Myc^P0 in heterozygous state and the increased number of mitotic cells can be suppressed by combination with Myc^G0354/+.

The number of mitotic cells in the wing disc of hfp^EP3058/hfp^EP3058;hay^nc2/+ third instar larvae is not changed by combination with Myc^G0354 in heterozygous state.

(Lee et al., 2015)

The female sterility and oogenesis phenotypes of dm^P0 flies are suppressed by pUf68^EP3058/+. The block in cycle 14 mitosis and increased cell-size in cycle 14 embryos seen in stg^AR2/stg^AR2 embryos is suppressed by pUf68^EP3058/pUf68^EP3058. Unlike stg^AR2 homozygotes, stg^AR2; pUf68^EP3058 double homozygotes survive embryogenesis and die between first and second instar.

(Quinn et al., 2004)

Xenogenetic Interactions

Statement

Reference

The small, rough eye phenotype of Hsap\CDKN1A^GMR.PH flies is partially suppressed by pUf68^EP3058/+ (the eyes are larger and contain fewer fused ommatidia).

(Quinn et al., 2004)

Complementation and Rescue Data

Partially rescued by

hfp^EP3058 is partially rescued by Scer\GAL4^arm.PU, hfp^EP3058

(Lee et al., 2015)

Partially rescues

Scer\GAL4^arm.PU, hfp^EP3058 partially rescues hfp^EP3058

(Lee et al., 2015)

Comments

The increased body size characteristic for hfp^EP3058 homozygous third instar larvae can be suppressed by driving hfp expression from the UAS included in the hfp^EP3058 P-element insertion allele under the control of the Scer\GAL4^arm.PU driver.

(Lee et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

109011

w^*; P{EP}hfp^EP3058 / TM6B, Tb¹

Notes on Origin

Discoverer

Separable from: additional background lethal mutations.

(Van Buskirk and Schupbach, 2002)

Comments

The original strain contained second site lethals which were recombined off during this work.

(Quinn et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

EP(3)3058

(Van Buskirk and Schupbach, 2002)

Hfp^EP

(Lee et al., 2015)

hfp^EP3058

hfp^EP

(Quinn et al., 2004)

pUbsf^EP3058

pUf68^EP3058

puf68^EP3058

(Sofola et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close