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General Information
Symbol
Hsap\CDKN1AGMR.PH
Species
H. sapiens
Name
glass multimer reporter construct of Hariharan
FlyBase ID
FBal0047094
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
GMR-p21, GMRp21
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

GMR regulatory sequences drive expression of a Hsap\CDKN1A cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\CDKN1AGMR.PH in the developing eye results in ommatidial fusion.

Expression of Hsap\CDKN1AGMR.PH produces flies with rough eyes. Little or no apoptosis is seen in third instar eye discs and differentiation of photoreceptors is largely normal.

Hsap\CDKN1AGMR.PH flies have small, rough eyes.

Flies expressing Hsap\CDKN1AGMR.PH have rough eyes. The second mitotic wave is absent in eye imaginal discs expressing Hsap\CDKN1AGMR.PH.

Flies expressing Hsap\CDKN1AGMR.PH have a severe rough eye phenotype. The number of cells entering S phase posterior to the morphogenetic furrow is severely reduced compared to wild-type.

Ommatidial architecture is irregular, and interommatidial bristle number is reduced to 15% of wild type. Almost all (more than 98%) of ommatidia have the full complement of photoreceptor cells, though only 13% were fully surrounded by pigment cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

Hsap\CDKN1AGMR.PH has phenotype, non-enhanceable by osaE65

Suppressed by
Statement
Reference

Hsap\CDKN1AGMR.PH has ommatidium phenotype, suppressible | partially by pUf68[+]/hfpEP3058

Hsap\CDKN1AGMR.PH has eye phenotype, suppressible | partially by pUf68[+]/hfpEP3058

Hsap\CDKN1AGMR.PH has eye disc & mitotic cell cycle phenotype, suppressible | partially by CycEGMR.PB

Hsap\CDKN1AGMR.PH has eye disc & mitotic cell cycle phenotype, suppressible | partially by ebiunspecified/ebi[+]

Hsap\CDKN1AGMR.PH has eye phenotype, suppressible by ebiunspecified/ebi[+]

Hsap\CDKN1AGMR.PH has ommatidium phenotype, suppressible by ebiunspecified/ebi[+]

Hsap\CDKN1AGMR.PH has eye phenotype, suppressible by Df(1)AD11

Hsap\CDKN1AGMR.PH has eye phenotype, suppressible by Df(1)su(s)83

Hsap\CDKN1AGMR.PH has eye phenotype, suppressible by Rbf120a/Rbf[+]

Hsap\CDKN1AGMR.PH has eye phenotype, suppressible by Rbf14/Rbf[+]

NOT suppressed by
Statement
Reference

Hsap\CDKN1AGMR.PH has eye phenotype, non-suppressible by Cdk7S164A.T170A

Hsap\CDKN1AGMR.PH has eye disc & mitotic cell cycle phenotype, non-suppressible by ttk88.hs

Hsap\CDKN1AGMR.PH has phenotype, non-suppressible by Diap1SL

Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

A Cdk7S164A.T170A mutant background does not suppress the ommatidial fusion found upon expression of Hsap\CDKN1AGMR.PH in the developing eye.

Expression of Hsap\CDKN1AGMR.PH rescues the cell death and R7 photoreceptor loss seen in p53GMR.Ex third instar larval eye discs. The adult eye ablation phenotype is also rescued.

The increased eye pigmentation in bwD/+ heterozygotes resulting from the mis-expression of Acf1EY08629 driven by Scer\GAL4GMR.PF is not affected by Hsap\CDKN1AGMR.PH.

The increased eye pigmentation in bwD/+ heterozygotes resulting from the mis-expression of banunspecified driven by Scer\GAL4GMR.PF is suppressed by Hsap\CDKN1AGMR.PH.

The increased eye pigmentation in bwD/+ heterozygotes resulting from the mis-expression of crolunspecified driven by Scer\GAL4GMR.PF is suppressed by Hsap\CDKN1AGMR.PH.

The small, rough eye phenotype of Hsap\CDKN1AGMR.PH flies is partially suppressed by hfpEP3058/+ (the eyes are larger and contain fewer fused ommatidia). Compared with Hsap\CDKN1AGMR.PH, eye discs from Hsap\CDKN1AGMR.PH; hfpEP3058/+ animals have more S-phase cells posterior to the morphogenetic furrow, and an accompanying increase in mitotic cells. The small, rough eye phenotype of Hsap\CDKN1AGMR.PH flies is enhanced by MycP0/+.

The small, rough eye phenotype of Hsap\CDKN1AGMR.PH flies is partially suppressed by pUf68EP3058/+ (the eyes are larger and contain fewer fused ommatidia). Compared with Hsap\CDKN1AGMR.PH, eye discs from Hsap\CDKN1AGMR.PH; , pUf68EP3058/+ animals have more S-phase cells posterior to the morphogenetic furrow, and an accompanying increase in mitotic cells. The small, rough eye phenotype of Hsap\CDKN1AGMR.PH flies is enhanced by dmP0/+.

Expression of Hsap\CDKN1AGMR.PH in homozygous EgfrE3 mutants does not prevent the apoptosis of undifferentiated cells in the eye disc that is seen in the EgfrE3 mutant background.

The Hsap\CDKN1AGMR.PH eye phenotype is dominantly suppressed by the addition of ebiunspecified. The resulting eyes are almost wild-type, showing minor bristle defects. ebiunspecified also partially rescues the loss of the second mitotic wave usually seen in Hsap\CDKN1AGMR.PH eye discs.

The eye phenotype produced by Hsap\CDKN1AGMR.PH is strongly suppressed by Df(1)AD11, Df(1)su(s)83, Rbf120a or Rbf14.

The Hsap\CDKN1AGMR.PH rough eye phenotype is suppressed by spenpoc361. The second mitotic wave is partially restored in Hsap\CDKN1AGMR.PH flies the presence of one copy of spenpoc361.

Hsap\CDKN1AGMR.PH;CycEJP double mutant flies have a more severe rough eye phenotype and greater reduction in the number of cells entering S phase posterior to the morphogenetic furrow than either Hsap\CDKN1AGMR.PH or CycEJP single mutant flies.

Expression of Hsap\CDKN1AGMR.PH, which blocks the second mitotic wave, in Gl1 mutants substantially reduces the number of mispositioned nuclei. Expression also causes substantial cell death behind the morphogenetic furrow in Gl1 mutants.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\CDKN1AGMR.PH
glass-p21
Name Synonyms
glass multimer reporter construct of Hariharan
Secondary FlyBase IDs
    References (19)