FlyBase Allele Report: Dmel\hay[nc2rv7]

General Information

Symbol

Dmel\hay^nc2rv7

Species

D. melanogaster

Name

FlyBase ID

FBal0005391

Feature type

allele

Associated gene

Dmel\hay

Associated Insertion(s)

Carried in Construct

Also Known As

hay^rv7

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Regan and Fuller, 1990)

Mutagen

ethyl methanesulfonate

(Regan and Fuller, 1990)

Progenitor genotype

hay^nc2

(Mounkes and Fuller, 1999, Regan and Fuller, 1990)

Cytology

Description

Mutation lies 6 amino acids before the magnesium-binding domain (helicase domain II).

(Mounkes and Fuller, 1999)

Nucleotide substitution: G?A.

(Mounkes and Fuller, 1999)

Amino acid replacement: W441term.

(Mounkes and Fuller, 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:10,632,778..10,632,778 [+]

Nucleotide change:

G10632778A

Reported nucleotide change:

G?A

Amino acid change:

W437term | hay-PA; W437term | hay-PB

Reported amino acid change:

W441term

Comment:

The amino acid numbering differs between FBrf0108244 and the reference sequence. The positions and nature of the base substitutions in the mutants are derived from the sequence in Fig. 1 and from Table 1, and provide unambiguous assignments.

(Mounkes and Fuller, 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | male | recessive

(Regan and Fuller, 1990)

lethal

(Mounkes and Fuller, 1999)

lethal | recessive

(Aguilar-Fuentes et al., 2008, Regan and Fuller, 1990)

partially lethal (with hay^nc2)

(Merino et al., 2002)

visible (with hay^nc2)

(Merino et al., 2002)

Phenotype Manifest In

adult abdomen & endocuticle (with hay^nc2)

(Merino et al., 2002)

macrochaeta (with hay^nc2)

(Merino et al., 2002)

microchaeta (with hay^nc2)

(Merino et al., 2002)

wing (with hay^nc2)

(Merino et al., 2002)

wing margin (with hay^nc2)

(Merino et al., 2002)

Detailed Description

Statement

Reference

hay^nc2rv7/+ mutants do not show any changes in the rate of cell proliferation in the third instar larval wing discs compared to wild-type controls.

(Lee et al., 2015)

Only small homozygous clones are recovered in the third instar larval eye disc.

(Matsuno et al., 2007)

hay^nc2/hay^nc2rv7 animals show 38.8% viability. 46.1% of hay^nc2/hay^nc2rv7 adults have deformed and fragile macrochaetae and microchaetae, 16.7% have a reduction of the endocuticular layer of the abdominal cuticle and 1.0% show loss of wing marginal regions and blistered wings.

(Merino et al., 2002)

Lethal over deficiency of hay. Heterozygotes exhibit defects in the structure and function of the male meiotic spindle.

(Regan and Fuller, 1990)

recessive male-sterile

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

hay^nc2rv7 has lethal | recessive phenotype, non-suppressible by Tfb5^Hsp83.PAF

(Aguilar-Fuentes et al., 2008)

Enhancer of

Statement

Reference

hay[+]/hay^nc2rv7 is an enhancer of increased body size | third instar larval stage phenotype of hfp^EP3058

(Lee et al., 2015)

hay[+]/hay^nc2rv7 is an enhancer of increased occurrence of cell division | third instar larval stage phenotype of hfp^EP3058

(Lee et al., 2015)

Other

Statement

Reference

Cdk7^P140S, hay[+]/hay^nc2rv7 has visible phenotype

(Merino et al., 2002)

Cdk7^P140S, hay^nc2rv7 has visible phenotype

(Merino et al., 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

hay[+]/hay^nc2rv7 is an enhancer of wing disc | third instar larval stage phenotype of hfp^EP3058

(Lee et al., 2015)

Other

Statement

Reference

Cdk7^P140S, hay[+]/hay^nc2rv7 has chaeta phenotype

(Merino et al., 2002)

Cdk7^P140S, hay^nc2rv7 has chaeta phenotype

(Merino et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The large body size as well as the higher number of mitotic cells in the wing disc characteristic for the hfp^EP3058/hfp^EP3058 third instar larvae is further increased by combination with hay^nc2rv7 in heterozygous state.

(Lee et al., 2015)

Flies carrying Cdk7^P140S in a heterozygous Df(1)JB254 P{snf⁺,dhd⁺} background (the Df(1)JB254 chromosome is deficient for Cdk7, snf and dhd and snf and dhd function is provided by the P{snf⁺,dhd⁺} transgene) at the restrictive temperature that are also carrying one copy of hay^nc2rv7 have brittle bristles.

(Merino et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Hay^RV7

(Lee et al., 2015)

Hay^nc2rv7

(Lee et al., 2015)

hay^nc2rv7

(Aguilar-Fuentes et al., 2008)

hay^rv7

(Matsuno et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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